Dental plaque development on a hydroxyapatite disk in young adults observed by using a barcoded pyrosequencing approach

Takeshita, Toru; Yasui, Masaki; Shibata, Yukie; Furuta, Michiko; Saeki, Yoji; Eshima, Nobuoki; Yamashita, Yoshihisa

doi:10.1038/srep08136

Cited by 54 publications

(66 citation statements)

References 26 publications

(34 reference statements)

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“…1 It has previously been confirmed that there are substantial differences between planktonic bacteria and bacteria in biofilm and that biofilm has a more important role in oral health. 2,3 However, experiments with real oral biofilm are difficult because it is hard to control the human oral environment, and the oral microbiota of the biofilms of each human also exhibit substantial differences.…”

Section: Introductionmentioning

confidence: 99%

“…2,3 However, experiments with real oral biofilm are difficult because it is hard to control the human oral environment, and the oral microbiota of the biofilms of each human also exhibit substantial differences. 1 Therefore, an in vitro model that mimics real oral biofilm is needed to understand the complexities of biofilm. Microcosm biofilm models have been suggested to overcome such limitations, 4,5 and some researchers have reported that these in vitro models are similar to real oral biofilms.…”

Section: Introductionmentioning

confidence: 99%

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Ecological changes in oral microcosm biofilm during maturation

et al. 2016

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Abstract. The aim of this study was to evaluate the ecological changes in the biofilm at different stages of maturation using 16S rDNA gene amplicon sequencing and to identify correlations between red/green (R/G) fluorescence ratio and ecological changes. An oral microcosm biofilm was initiated from the saliva of a single donor and grown anaerobically for up to 10 days in basal medium mucin. Quantitative light-induced fluorescence analysis was shown that the R/G ratio of the biofilm increased consistently, but the slope rapidly decreased after six days. The bacterial compositions of 10 species also consistently changed over time. However, there was no significant correlation between each bacteria and red fluorescence. The monitoring of the maturation process of oral microcosm biofilm over 10 days revealed that the R/G ratio and the bacterial composition within biofilm consistently changed. Therefore, the R/G fluorescence ratio of biofilm may be related with its ecological change rather than specific bacteria. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ecological changes in oral microcosm biofilm during maturation

et al. 2016

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“…It was recently reported that Capnocytophaga spp. increase their dominance within the bacterial community as dental plaque matures (57). Furthermore, a number of other bacteria, including major periodontal pathogens, have been found to harbor T9SS-related genes (28).…”

Section: Figmentioning

confidence: 99%

Involvement of the Type IX Secretion System in Capnocytophaga ochracea Gliding Motility and Biofilm Formation

Kita

Shibata

Kikuchi

et al. 2016

Appl Environ Microbiol

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dCapnocytophaga ochracea is a Gram-negative, rod-shaped bacterium that demonstrates gliding motility when cultured on solid agar surfaces. C. ochracea possesses the ability to form biofilms; however, factors involved in biofilm formation by this bacterium are unclear. A type IX secretion system (T9SS) in Flavobacterium johnsoniae was shown to be involved in the transport of proteins (e.g., several adhesins) to the cell surface. Genes orthologous to those encoding T9SS proteins in F. johnsoniae have been identified in the genome of C. ochracea; therefore, the T9SS may be involved in biofilm formation by C. ochracea. Here we constructed three ortholog-deficient C. ochracea mutants lacking sprB (which encodes a gliding motility adhesin) or gldK or sprT (which encode T9SS proteins in F. johnsoniae). Gliding motility was lost in each mutant, suggesting that, in C. ochracea, the proteins encoded by sprB, gldK, and sprT are necessary for gliding motility, and SprB is transported to the cell surface by the T9SS. For the ⌬gldK, ⌬sprT, and ⌬sprB strains, the amounts of crystal violet-associated biofilm, relative to wild-type values, were 49%, 34%, and 65%, respectively, at 48 h. Confocal laser scanning and scanning electron microscopy revealed that the biofilms formed by wild-type C. ochracea were denser and bacterial cells were closer together than in those formed by the mutant strains. Together, these results indicate that proteins exported by the T9SS are key elements of the gliding motility and biofilm formation of C. ochracea.

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“…Temporal changes during supragingival plaque formation vary among individuals (15). Therefore, if a representative sample of plaque is required on one occasion, plaque maturity should be determined.…”

Section: What Is the Oral Microbiome?mentioning

confidence: 99%

The oral microbiome and human health

Yamashita

Takeshita

2017

Journal of Oral Science

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258

152

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Abstract:In this brief review, we discuss our previous research on the relationship between the bacterial composition of salivary microbiota and periodontal disease. Analysis using a terminal restriction fragment length polymorphism method and an international comparison suggest that the predominance of the genera Prevotella and Veillonella in the salivary microbiota is attributable to periodontal disease conditions, and that the predominance of the genus Neisseria indicates healthy periodontal conditions. Furthermore, we recently used next-generation sequencing technology to perform a detailed largescale analysis of the salivary microbiota. An important finding of that study was that high bacterial richness in the salivary microbiota was significantly associated with poor oral health, as indicated by decayed teeth, periodontitis, and poor oral hygiene. Another important result was that relative abundance of predominant bacteria in saliva was significantly associated with oral health-related conditions. Of the two different cohabiting groups of bacteria found in the salivary microbiota, a greater relative abundance of group I bacteria, which include Prevotella and Veillonella species, was associated with poor oral health, high body mass index, and old age. These findings suggest that the salivary microbiota reflects oral and systemic conditions.

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Dental plaque development on a hydroxyapatite disk in young adults observed by using a barcoded pyrosequencing approach

Cited by 54 publications

References 26 publications

Ecological changes in oral microcosm biofilm during maturation

Ecological changes in oral microcosm biofilm during maturation

Involvement of the Type IX Secretion System in Capnocytophaga ochracea Gliding Motility and Biofilm Formation

The oral microbiome and human health

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