Deoxycholic acid-conjugated chitosan oligosaccharide nanoparticles for efficient gene carrier

Chae, Su Young; Son, Sohee; Lee, Minhyung; Jang, Mi-Kyeong; Nah, Jae‐Woon

doi:10.1016/j.jconrel.2005.09.040

Cited by 184 publications

(83 citation statements)

References 36 publications

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“…In the past, DC-modified chitosan was reported for self-aggregated nanoparticles and its potential as a gene-delivery carrier. 23,24 In accordance with our consideration, the conjugation with DC not only obstructs the H-bond but also provides the hydrophobicity for the compatibility with polyethylene.…”

Section: Introductionsupporting

confidence: 79%

Chitosan conjugated with deoxycholic acid and gallic acid: A novel biopolymer‐based additive antioxidant for polyethylene

Pasanphan

Buettner

Chirachanchai

2008

J of Applied Polymer Sci

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Chitosan, as a novel bioadditive antioxidant for commodity polymer, is proposed. Chitosan is successfully conjugated with deoxycholic acid and gallic acid (chitosan-DC-GA) via a simple reaction using the 1-ethyl-3-(3 0 -dimethylaminopropyl) carbodiimide/Nhydroxysuccinimide conjugating agent at room temperature in a heterogeneous system. Chitosan-DC-GA shows a deoxycholic acid (DC) and gallic acid (GA) content of about 40% each. Chitosan-DC-GA exhibits DPPH radical scavenging ability with EC 50 1 mg mL 21 based on the EPR technique evaluation. A model case study of chitosan-DC-GA with low-density polyethylene (LDPE) confirms improvement in compatibility, as seen from the sheet clarity, as well as the good dispersion of chitosan-DC-GA in LDPE matrices, as observed by scanning electron microscope.

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Section: Introductionsupporting

confidence: 79%

Chitosan conjugated with deoxycholic acid and gallic acid: A novel biopolymer‐based additive antioxidant for polyethylene

Pasanphan

Buettner

Chirachanchai

2008

J of Applied Polymer Sci

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“…The optical density (OD) value was measured at 492 nm using a microplate reader. The percentage of anticancer activity was calculated according to the following equation [46]: Anticancer activity (%) = (OD 492, control − OD 492, sample )/(OD 492, control − OD 492, blank ) × 100 (4) where OD 492, sample was the measurement from the docetaxel injection, blank SA-BSPs copolymer micelles, and DTX-SA-BSPs copolymer micelles; OD 492, control was the measurement from the cells treated with incubated solution; and OD 492, blank was the incubated solution.…”

Section: In Vitro Anticancer Activitymentioning

confidence: 99%

Docetaxel-Loaded Self-Assembly Stearic Acid-Modified Bletilla striata Polysaccharide Micelles and Their Anticancer Effect: Preparation, Characterization, Cellular Uptake and In Vitro Evaluation

et al. 2016

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Poorly soluble drugs have low bioavailability after oral administration, thereby hindering effective drug delivery. A novel drug-delivery system of docetaxel (DTX)-based stearic acid (SA)-modified Bletilla striata polysaccharides (BSPs) copolymers was successfully developed. Particle size, zeta potential, encapsulation efficiency (EE), and loading capacity (LC) were determined. The DTX release percentage in vitro was determined using high performance liquid chromatography (HPLC). The hemolysis and in vitro anticancer activity were studied. Cellular uptake and apoptotic rate were measured using flow cytometry assay. Particle size, zeta potential, EE and LC were 125.30 ± 1.89 nm, −26.92 ± 0.18 mV, 86.6% ± 0.17%, and 14.8% ± 0.13%, respectively. The anticancer activities of DTX-SA-BSPs copolymer micelles against HepG2, HeLa, SW480, and MCF-7 (83.7% ± 1.0%, 54.5% ± 4.2%, 48.5% ± 4.2%, and 59.8% ± 1.4%, respectively) were superior to that of docetaxel injection (39.2% ± 1.1%, 44.5% ± 5.3%, 38.5% ± 5.4%, and 49.8% ± 2.9%, respectively) at 0.5 µg/mL drug concentration. The DTX release percentage of DTX-SA-BSPs copolymer micelles and docetaxel injection were 66.93% ± 1.79% and 97.06% ± 1.56% in two days, respectively. Cellular uptake of DTX-FITC-SA-BSPs copolymer micelles in cells had a time-dependent relation. Apoptotic rate of DTX-SA-BSPs copolymer micelles and docetaxel injection were 73.48% and 69.64%, respectively. The SA-BSPs copolymer showed good hemocompatibility. Therefore, SA-BSPs copolymer can be used as a carrier for delivering hydrophobic drugs.

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“…14 Recently our studies are focused on chemical modifications, nanoparticle preparation, and drug-release behaviors of low molecular weight water soluble chitosan (LMWSC). [15][16][17] In the present study, we have designed novel thermosensitive nanoparticles, based on NIPAAm-co-AA core exhibiting LCST behaviors and chitosan shell having biocompatibility, to develop the controlled release of the loaded drug. Chitosan conjugated NIPAAm-co-AA nanoparticles with advantages of temperature sensitivity and biodegradability were synthesized.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of thermosensitive nanoparticles based on PNIPAAm core and chitosan shell structure

et al. 2009

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Noble thermosensitive nanoparticles, based on a PNIPAAm-co-AA core and a chitosan shell structure, were designed and synthesized for the controlled release of the loaded drug. PNIPAAm nanoparticles containing a carboxylic group on their surface were synthesized using emulsion polymerization. The carboxylic groups were conjugated with the amino group of a low molecular weight, water soluble chitosan. The particle size of the synthesized nanoparticles was decreased from 380 to 25 nm as the temperature of the dispersed medium was increased. Chitosan-conjugated nanoparticles with 2~5 wt% MBA, a crosslinking monomer, induced a stable aqueous dispersion at a concentration of 1 mg/1 mL. The chitosan-conjugated nanoparticles showed thermosensitive behaviors such as LCST and size shrinkage that were affected by the PNIPAAm core and induced some particle aggregation around LCST, which was not shown in the NIPAAm-co-AA nanoparticles. These chitosan-conjugated nanoparticles are also expected to be more biocompatible than the PNIPAAm core itself through the chitosan shell structures.

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Deoxycholic acid-conjugated chitosan oligosaccharide nanoparticles for efficient gene carrier

Cited by 184 publications

References 36 publications

Chitosan conjugated with deoxycholic acid and gallic acid: A novel biopolymer‐based additive antioxidant for polyethylene

Chitosan conjugated with deoxycholic acid and gallic acid: A novel biopolymer‐based additive antioxidant for polyethylene

Docetaxel-Loaded Self-Assembly Stearic Acid-Modified Bletilla striata Polysaccharide Micelles and Their Anticancer Effect: Preparation, Characterization, Cellular Uptake and In Vitro Evaluation

Synthesis and characterization of thermosensitive nanoparticles based on PNIPAAm core and chitosan shell structure

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