Deoxyribonucleic Acid Base Composition in Certain Bacteria and Their Mutants with Impaired Respiration

Gause, G.G.; Loshkareva, N.P.; Zbarsky, I. B.; Gause, G. F.

doi:10.1038/203598a0

Cited by 31 publications

(7 citation statements)

References 9 publications

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“…Theoretically, it is conceivable, however, that these organisms might be mutants with altered base compositions. Mutants with altered G + C ratios have been described (Weed, 1963;Gause et al, 1964;DeLey, 1964).…”

Section: Resultsmentioning

confidence: 99%

Heterogeneity Among the Mycoplasma and Relationships to Bacteria*

Neimark

1967

Annals of the New York Academy of Sciences

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Section: Resultsmentioning

confidence: 99%

Heterogeneity Among the Mycoplasma and Relationships to Bacteria*

Neimark

1967

Annals of the New York Academy of Sciences

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“…On the other hand, the induction of RD mutants by 4-NQO was caused rather by the change in DNA base composition. It is interesting in connection with this that guanine-cytosine contents of DNA in RD mutants of various groups of micro-organisms induced by 5-fluorouracil, 5-bromouracil and copper treatments were higher than those of parental DNA (3). From the fact that cytoplasmic elements such as F-factor and R-factor consist of DNA, it can not be said that 4-NQO has no effect on the cytoplasmic elements.…”

Section: Discussionmentioning

confidence: 94%

EFFECT OF METHYLENE BLUE ON THE ACTION OF 4‐NITROQUINOLINE N‐OXIDE AND ACRIFLAVINE IN INDUCING RESPIRATION‐DEFICIENT MUTANTS OF SACCHAROMYCES CEREVISIAE

Morita

Mifuchi

1965

Japanese Journal of Microbiology

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Since a respiration-deficient(RD) mutant of yeast was first obtained by Ephrussi et al. (2) by treatment with acriflavine(AF) and named ' petite colonie ' mutant, others have been induced by many basic dyes'13), enzyme inhibitors(26), heavy metal salts(8), and physical effects such as ultraviolet irradiation (23) and heat shock(27).In previous papers(9,10) we reported that a carcinogenic agent, 4-nitroquinoline N-oxide(4-NQO), could induce an RD mutant which was seemingly like the mutant induced by AF. From the viewpoint of induction rate and the time required for the induction of RD mutants, the effects of 4-NQO and AF were unlike. The mutants produced by these two agents differed in their cytochrome content and in enzyme activities such as catalase, succinic dehydrogenase, lactic dehydrogenase, etc. (12). Consequently, it was thought that the mechanisms of the induction of RD mutants by both agents might be different.Extensive studies (7,14,15,17,24) on the mechanism of the induction of RD mutants with AF have been made which suggest the elimination of cytoplasmic elements and the change of nucleic acid base components as the action of AF. Nagai (15) found that the induction of RD mutants by AF was counteracted by methylene blue and toluidine blue, and from his results he suggested that AF exerted its RDinducing action in a certain specific mode of binding between the dye molecules and receptor material of yeast cells.The present paper deals with the difference in the effect of methylene blue on the induction of RD mutants by 4-NQO and AF. MATERIALS AND METHODS

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“…The significance of this wide range of values in a single species is not apparent. The differential value of GC-content determinations in the Micrococcaceae is in more serious jeopardy if mutants of S. aureus with GC contents of 71% exist, as reported by Gause (8). Substantial change in base composition of an organism has also been reported in Bacillus by Weed (20) and in some very early work on Escherichia by Spirin et al (4).…”

mentioning

confidence: 87%

“…The observed 2.7% range in GC content of S. aureus under this variety of conditions represents the limit of error of the technique and the range of the total DNA molecular population. The apparent exception to GC constancy, the UV-2 "mutant" of S. aureus ATCC 13679 (8), is really a species of Corynebacterium.…”

Section: Cationmentioning

confidence: 99%

Deoxyribonucleic Acid Base Composition in the Taxonomy of Staphylococcus

1969

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Twenty-four strains of Staphylococcus aureus, including eight known mutants of S. aureus and strains growing under a variety of environmental conditions or exposed to a number of physical and chemical agents, maintained a remarkably narrow range of guanine plus cytosine (GC) content (32.4 to 35.1 %). The wide range of GC content (30.7 to 40%) reported in the literature was due to the variety of methods and calculations used rather than to any substantial variation in base composition. The UV-2 "mutant" (ATCC 13680) with a GC content of 67.6% reported to be derived from S. aureus (ATCC 13679) was a species of Corynebacterium. The data presented were consistent with the concept that base composition changes only to a very slight degree by mutation.

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Deoxyribonucleic Acid Base Composition in Certain Bacteria and Their Mutants with Impaired Respiration

Cited by 31 publications

References 9 publications

Heterogeneity Among the Mycoplasma and Relationships to Bacteria*

Heterogeneity Among the Mycoplasma and Relationships to Bacteria*

EFFECT OF METHYLENE BLUE ON THE ACTION OF 4‐NITROQUINOLINE N‐OXIDE AND ACRIFLAVINE IN INDUCING RESPIRATION‐DEFICIENT MUTANTS OF SACCHAROMYCES CEREVISIAE

Deoxyribonucleic Acid Base Composition in the Taxonomy of Staphylococcus

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