Deproteinization as a Rapid Method of Saliva Purification for the Determination of Carbamazepine and Carbamazepine-10,11 Epoxide

Dziurkowska, Ewelina; Wesołowski, Marek

doi:10.3390/jcm9040915

Cited by 9 publications

(8 citation statements)

References 26 publications

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“…Despite the advantages mentioned above, this substance has a lot of destructive side effects, such as nausea, vomiting, dizziness, drowsiness, etc. Considering this context, it can be concluded that the determination of Carba is very important. , Therefore, it is important to develop selective, rapid, appropriate, and accurate methods for determining trace amounts of drugs . Given the importance of measuring each drug individually and the structural and size similarity of these two combinations, their simultaneous measurement is of great importance.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: CdSe Quantum Dot Nanoparticles: Synthesis and Application in the Development of Molecularly Imprinted Polymer-Based Dual Optical Sensors

Davoodi

Davar

Mandani

et al. 2021

Ind. Eng. Chem. Res.

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In this study, two types of CdSe, including nanoparticles and quantum dots, were synthesized. CdSe quantum dot nanoparticles were prepared via the hydrothermal technique at 140 °C for 8 h. The prepared CdSe materials were used as fluorimetric sensors aimed at the simultaneous analysis and detection of methamphetamine (Metha) as well as carbamazepine (Carba) drugs. Besides, the fluorimetric sensor was assembled depending on molecularly imprinted polymer (MIP) films with two sizes of cadmium selenide quantum dots as fluorophores source. The mechanistic synthesis of the fluorimetric sensor was based on covering the CdSe quantum dot nanoparticle surfaces with MIP film; afterward, a mixture of powdered yellow-CdSe@MIPs (y-CdSe@MIPs) and red-CdSe@MIPs (r-CdSe@MIPs) nanocomposites was applied for the simultaneous optical determination of Metha and Carba. Under optimal conditions, detection limits (DL) of 0.15 and 0.2 nM, as well as linear dynamic ranges (LDR) of 0.5 and 260 nM were acquired for Metha and Carba, respectively. Additionally, the application of the offered optical dual sensor was studied in human blood plasma as well as urine analytes.

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Section: Introductionmentioning

confidence: 99%

RETRACTED: CdSe Quantum Dot Nanoparticles: Synthesis and Application in the Development of Molecularly Imprinted Polymer-Based Dual Optical Sensors

Davoodi

Davar

Mandani

et al. 2021

Ind. Eng. Chem. Res.

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“…There are different methods (GC, LC, HPLC, High Performance Thin Layer Chromatography (HPTLC), LC-MS/MS, UHPLC-MS/MS, SFC-ESI-MS/MS) to measure the level of CBZ and its major metabolite in biological samples of epilepsy patients treated with this AED in monotherapy or polytherapy. The presented methods use biological samples such as: Plasma [47][48][49][50][51][52][53][54][55], serum [53,[56][57][58], urine [47,59], saliva [60], dried saliva [61] and respiratory condensate [62,63], dried blood spot [20,64,65]. In TDM of CBZ, plasma, serum, and urine are the most commonly used.…”

Section: Carbamazepinementioning

confidence: 99%

“…In the dried blood spot method, Whatman 903 Protein Saver Cards and the LC with triple quadrupole mass chromatography were used [64]. Saliva is particularly useful in children, and the correlation between blood and saliva CBZ and its metabolite concentration was confirmed [60]. The described methods differ in the sample preparation technique including liquid-liquid extraction (LLE) [53,55,56,61], ultrasound-assisted emulsification microextraction (SAEME) [47,50], microextraction by packed sorbent (MEPS) [55,66] and deproteinization [48,49,57,58].…”

Section: Carbamazepinementioning

confidence: 99%

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New Methods Used in Pharmacokinetics and Therapeutic Monitoring of the First and Newer Generations of Antiepileptic Drugs (AEDs)

et al. 2020

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The review presents data from the last few years on bioanalytical methods used in therapeutic drug monitoring (TDM) of the 1st–3rd generation and the newest antiepileptic drug (AEDs) cenobamate in patients with various forms of seizures. Chemical classification, structure, mechanism of action, pharmacokinetic data and therapeutic ranges for total and free fractions and interactions were collected. The primary data on bioanalytical methods for AEDs determination included biological matrices, sample preparation, dried blood spot (DBS) analysis, column resolution, detection method, validation parameters, and clinical utility. In conclusion, the most frequently described method used in AED analysis is the LC-based technique (HPLC, UHPLC, USLC) combined with highly sensitive mass detection or fluorescence detection. However, less sensitive UV is also used. Capillary electrophoresis and gas chromatography have been rarely applied. Besides the precipitation of proteins or LLE, an automatic SPE is often a sample preparation method. Derivatization was also indicated to improve sensitivity and automate the analysis. The usefulness of the methods for TDM was also highlighted.

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“…There are few analytical methods that are reported in literature that quantify CBZ in saliva. Among these, some use methodologies not common in many laboratories, such as UHPLC [18,21], and HPTLC [2]. Other studies show the application of the HPLC technique but do not describe the development and validation of the method [7,9,10].…”

Section: Introductionmentioning

confidence: 99%

A Validated Lc Method for Determination of Carbamazepine in Saliva

De-Diego¹,

Mennickent²,

Pino³

et al. 2022

J. Chil. Chem. Soc.

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Carbamazepine (CBZ) is a drug used as anticonvulsant, especially in the treatment of epilepsy. For adequate control disease, treatment must achieve adequate blood concentrations, therefore, it is very important to control the levels of anticonvulsant drugs in biological matrices with suitable analytical methods. A simple and fast liquid chromatographic method with UV detection was developed and validated for the determination of CBZ in saliva. Chromatographic separation was achieved with a RP-18 column, using acetonitrile and water with triethylamine at pH 7,3 as mobile phase in isocratic elution mode, at a flow rate of 1 mL/min. The detection was done at 230 nm. and bromazepam at 1,5 µg/mL was used as IS. The run time was 5 min. The described method was linear over a range of 0,5 -5,5 g/mL. The intraassay and interassay precision, expressed as the RSD, were in the range of 0,67 -2,93 % and 0,17 -9,33 %, respectively, the extraction recoveries were between 100,67 -100,69 %, the accuracy values ranged from -3,33 to 0,69 % and -2,67 to 0,72 in intraday and interday analysis, respectively, and the assay demonstrated adequate selectivity and specificity. The LLOQ is below the therapeutic level, demonstrating an adequate sensitivity of the method. The results showed that the proposed method was found to be suitable for quantitative determination of CBZ in saliva.

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Deproteinization as a Rapid Method of Saliva Purification for the Determination of Carbamazepine and Carbamazepine-10,11 Epoxide

Cited by 9 publications

References 26 publications

RETRACTED: CdSe Quantum Dot Nanoparticles: Synthesis and Application in the Development of Molecularly Imprinted Polymer-Based Dual Optical Sensors

RETRACTED: CdSe Quantum Dot Nanoparticles: Synthesis and Application in the Development of Molecularly Imprinted Polymer-Based Dual Optical Sensors

New Methods Used in Pharmacokinetics and Therapeutic Monitoring of the First and Newer Generations of Antiepileptic Drugs (AEDs)

A Validated Lc Method for Determination of Carbamazepine in Saliva

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