SUMMARYThe responses of Norway spruce and Scots pine to the effects of increased O^ and Ca or Mg deficiency stress, singly and in combination, for two growing seasons, were investigated in this study. The combination of openair exposure to O. conctntrations of 1-3-1-6 times higher than ambient, and an inadequate supply of Ca or Mg, did nol affect root uptake and leachinf> of minerals from the needles of 3-yr-old seediings, indicating that they had not dexeloptd se\t'rf nutrient detiLifncy, Howevt-r, concentration of X decreased in the 1-yr-old needles of seedlings exposed to O.,. The height of the new shoots was promoted w ithout an increase in shttot dry weight, which together with stimulated mywirrhiza development and decreased shoot-to-root radio indicated allocation of carbon from shoots to roots as an early effect of low-le\'el O^ exposure. Although the ultrastructural study did not reveal clear O3 related injuries in cells, starch accumulated in chloroplasts ofthe fumigated seedlings grown with adequate, but not with deficient nutrients. The outcome of this study suggests that in medium-term exposure of conifer seedlings to low concentrations of ()., iind nutrient deficienc\, stress can be tolerated by activated defence metabolism and compensation reactions, but adverse effects may be apparent in long-term continuous exposure.