Derivation of Schwann cell precursors from neural crest cells resident in bone marrow for cell therapy to improve peripheral nerve regeneration

Shi, Haiyan; Gong, Yanpei; Qiang, Liang; Li, Xiaoli; Zhang, Shibo; Gao, Jiawen; Li, Kai; Ji, Ximeng; Tian, Ling; Gu, Xiaosong; Ding, Fei

doi:10.1016/j.biomaterials.2016.02.029

Cited by 28 publications

(23 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Dental pulp stem cells derived from human wisdom teeth have also been shown to resemble NCSC and were capable of differentiating into S100β‐expressing SC . Finally, human and rat bone marrow–derived NC‐like cells were also capable of differentiating into SC in vitro …”

Section: Ncscs: a Cell Source For The Treatment Of Demyelinating Disomentioning

confidence: 98%

“…These results highlight the tissue‐dependent differences in the abundance and self‐renewal capacity of adult NClSCs, which may ultimately determine the suitability for cellular therapies. Furthermore, bone marrow–derived NCs could be differentiated into SC that showed myelination around DRG neuronal axons in vitro, indicating their potential for use in peripheral nerve regeneration …”

Section: Sources Of Nc‐like Stem Cells In Adult Tissuesmentioning

confidence: 99%

“…166 Finally, human and rat bone marrow-derived NC-like cells were also capable of differentiating into SC in vitro. 11,167 Recently, our laboratory showed that cultures of KC from interfollicular epidermis could be the source of NClSCs (termed KC-NC), Specifically, these cells gave rise to BLBP+ glial cells in ovo and were localized around the axon bundles. Interestingly, KC-NC from aged donors maintained the same differentiation potential in vitro and in ovo, 32 indicating the potential of adult epidermis as a source of KC-NC for the treatment of neurodegenerative diseases.…”

Section: Adult Tissue-derived Nciscs For Schwann Cell Therapymentioning

confidence: 99%

“…Furthermore, bone marrow-derived NCs could be differentiated into SC that showed myelination around DRG neuronal axons in vitro, indicating their potential for use in peripheral nerve regeneration. 11 During development, NCs also migrate to the cornea, where their derivatives remain throughout adulthood. The cornea is a transparent avascular structure that covers the front of the eye and helps to focus light on the retina.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Adult tissue–derived neural crest-like stem cells: Sources, regulatory networks, and translational potential

Mehrotra

Tseropoulos

Bronner

et al. 2019

Stem Cells Translational Medicine

View full text Add to dashboard Cite

Neural crest (NC) cells are a multipotent stem cell population that give rise to a diverse array of cell types in the body, including peripheral neurons, Schwann cells (SC), craniofacial cartilage and bone, smooth muscle cells, and melanocytes. NC formation and differentiation into specific lineages takes place in response to a set of highly regulated signaling and transcriptional events within the neural plate border. Premigratory NC cells initially are contained within the dorsal neural tube from which they subsequently emigrate, migrating to often distant sites in the periphery. Following their migration and differentiation, some NC‐like cells persist in adult tissues in a nascent multipotent state, making them potential candidates for autologous cell therapy. This review discusses the gene regulatory network responsible for NC development and maintenance of multipotency. We summarize the genes and signaling pathways that have been implicated in the differentiation of a postmigratory NC into mature myelinating SC. We elaborate on the signals and transcription factors involved in the acquisition of immature SC fate, axonal sorting of unmyelinated neuronal axons, and finally the path toward mature myelinating SC, which envelope axons within myelin sheaths, facilitating electrical signal propagation. The gene regulatory events guiding development of SC in vivo provides insights into means for differentiating NC‐like cells from adult human tissues into functional SC, which have the potential to provide autologous cell sources for the treatment of demyelinating and neurodegenerative disorders.

show abstract

Section: Ncscs: a Cell Source For The Treatment Of Demyelinating Disomentioning

confidence: 98%

Section: Sources Of Nc‐like Stem Cells In Adult Tissuesmentioning

confidence: 99%

Section: Adult Tissue-derived Nciscs For Schwann Cell Therapymentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Adult tissue–derived neural crest-like stem cells: Sources, regulatory networks, and translational potential

Mehrotra

Tseropoulos

Bronner

et al. 2019

Stem Cells Translational Medicine

View full text Add to dashboard Cite

show abstract

“…[43][44][45] SCs participate in the production of NGF and differentiation into myelinating cells after peripheral nerve injury, and miRNA plays an important role in this procedure. 46,47 Forty-eight hours after the successful transfection of miR-221/222 into SCs, the expression of NGF and MBP in the miR-221/222 + group was significantly higher than that in the control group. The probable explanation is that miR-221/222 promoted SCs proliferation, and LASS2 is the target.…”

mentioning

confidence: 99%

Biodegradable and biocompatible cationic polymer delivering microRNA-221/222 promotes nerve regeneration after sciatic nerve crush

Song¹,

Li²,

Li³

et al. 2017

IJN

View full text Add to dashboard Cite

MicroRNA (miRNA) has great potential to treat a wide range of illnesses by regulating the expression of eukaryotic genes. Biomaterials with high transfection efficiency and low toxicity are needed to deliver miRNA to target cells. In this study, a biodegradable and biocompatible cationic polymer (PDAPEI) was synthetized from low molecular weight polyethyleneimine (PEI1.8kDa) cross-linked with 2,6-pyridinedicarboxaldehyde. PDAPEI showed a lower cytotoxicity and higher transfection efficiency than PEI25kDa in transfecting miR-221/222 into rat Schwann cells (SCs). The upregulation of miR-221/222 in SCs promoted the expression of nerve growth factor and myelin basic protein in vitro. The mouse sciatic nerve crush injury model was used to evaluate the effectiveness of PDAPEI/miR-221/222 complexes for nerve regeneration in vivo. The results of electrophysiological tests, functional assessments, and histological and immunohistochemistry analyses demonstrated that PDAPEI/miR-221/222 complexes significantly promoted nerve regeneration after sciatic nerve crush, specifically enhancing remyelination. All these results show that the use of PDAPEI to deliver miR-221/222 may provide a safe therapeutic means of treating nerve crush injury and may help to overcome the barrier of biomaterial toxicity and low efficiency often encountered during medical intervention.

show abstract

Peripheral Nerve Regeneration with 3D Printed Bionic Scaffolds Loading Neural Crest Stem Cell Derived Schwann Cell Progenitors

Yuan

et al. 2021

Adv Funct Materials

View full text Add to dashboard Cite

Peripheral nerve injuries are one of the most common types of traumatic damage to the nervous system. Treatment of peripheral nerve injuries aims to promote axon regrowth by imitating and improving the microenvironment for sciatic nerve regeneration. In this study, regeneration efficiency and behavior of peripheral nerves are compared under three treatment strategies: 1) transplantation of Schwann cell progenitors induced from purified neural crest stem cells; 2) implantation of a multiscale scaffold based on high‐resolution 3D printing; and 3) implantation of this bionic scaffold loading Schwann cell progenitors. The results of structural, electrophysiological, and behavioral tests demonstrate that the three treatment strategies result in different degrees of regeneration. The purified neural crest stem cells differentiate into functional Schwann cells and promote axon regeneration. The multifunctional 3D printed scaffold promotes oriented growth and myelination, and the myelinated nerve regrows with increased density and without visible scaffolds after six months. For the regeneration, scaffold treatment produces better performance than cell graft alone. Finally, it is shown that implantation of multiscale scaffolds preloaded with neural crest stem cell derived Schwann cell progenitors is the best strategy to promote peripheral nerve regeneration with improved anatomy and function among the three different strategies.

show abstract

Derivation of Schwann cell precursors from neural crest cells resident in bone marrow for cell therapy to improve peripheral nerve regeneration

Cited by 28 publications

References 55 publications

Adult tissue–derived neural crest-like stem cells: Sources, regulatory networks, and translational potential

Adult tissue–derived neural crest-like stem cells: Sources, regulatory networks, and translational potential

Biodegradable and biocompatible cationic polymer delivering microRNA-221/222 promotes nerve regeneration after sciatic nerve crush

Peripheral Nerve Regeneration with 3D Printed Bionic Scaffolds Loading Neural Crest Stem Cell Derived Schwann Cell Progenitors

Contact Info

Product

Resources

About