The present work evaluated the segregation of the RR-biotechnology and the agronomic performance of maize hybrids originated from three seed categories: single-cross commercial hybrids, double-cross hybrids, and saved seeds (F2 population). The experiment was set up in the field, within a producing area used for market purposes, in São Miguel do Iguaçu, Paraná, Brazil. The research followed a randomized block design with four repetitions. We investigated the following treatments: saved seeds/F2 population (MG 580 PW, MG 30A37 PW, MG 600 PW, and MG 545 PW), seeds of single-cross commercial hybrids (MG 580 PW, MG 30A37 PW, AS 1777 PRO3, AG 9000 PRO3, and DKB 290 PRO3) and home-bred seeds/double-cross hybrids (DKB 290 PRO3 x MG 30A37 PW, DKB 290 PRO3 x DKB 285 PEO3, DKB 290 PRO3 x DOW 2B587 PW, and DKB 290 PRO3 x MG 600 PW). The study considered agronomical characters, as well as the segregation of the RR-biotechnology. The hybrids MG 580 PW and DKB 290 PRO3 were the most productive ones, yielding 9,244.31 and 9,151.84 kg ha-1, respectively. The groups formed by double-cross and F2-population hybrids had the highest average values of RR-biotechnology segregation (20.5% and 13%, respectively). The group of commercial hybrids proved to be superior to the others in all variables appraised, and they also did not show segregation of the RR-biotechnology. The principal component analysis made it possible to sort the three categories of seeds. In conclusion, despite the high cost, the commercial seeds stood out showing the best cost-benefit for maize crops.