Design and characterization of a novel Arthrospira platensis glutathione oxido-reductase-derived antioxidant peptide GM15 and its potent anti-cancer activity via caspase-9 mediated apoptosis in oral cancer cells

Sannasimuthu, Anbazahan; Kumaresan, Venkatesh; Anilkumar, Shreya; Pasupuleti, Mukesh; Munuswamy-Ramanujam, Ganesh; Kundabala, M; Paray, Bilal Ahmad; Khalid, Muhammad; Albeshr, Mohammed Fahad; Arockiaraj, Jesu

doi:10.1016/j.freeradbiomed.2019.03.006

Cited by 53 publications

(45 citation statements)

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“…Total RNA was extracted from the collected A. platensis cells using TRIzol method as suggested by manufacture (Life Technologies, Rockville, MD, USA). Then the extracted total RNA was subjected to the conversion of cDNA using the cDNA synthesis kit [17]. Utilizing the cDNA as template, the ApAHc gene expression was evaluated in Light Cycle 96 Real Time PCR system using Fast SYBR Green Master Mix (Roche Diagnostics GmbH, Germany).…”

Section: Validation Of Apahc Gene Expression By Real Time Pcrmentioning

confidence: 99%

“…The ABTS (2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) radical assay was freshly prepared by dissolving ABTS (7 mM) in distilled water and by reacting it with potassium persulphate (2.4 mM) [23]. After 12-16 h, the reaction mixture was diluted with PBS (pH 7.4), peptide samples or Trolox were added at different concentrations (250 to 7.8 μM) and kept for 60 min at 30 °C [17]. The reading were taken in the Microplate reader at 734 nm.…”

Section: Abts Assaymentioning

confidence: 99%

“…The superoxide anion radical was produced [17] using 50 μl nitrotetrazolium blue chloride (2.52 mM), 50 μl NADH (624 mM) and different concentrations of peptide (250 to 7.8 µM). The reaction was started by including 50 μl phenazine methosulfate solution to the mixture.…”

Section: Superoxide Anion Radical Scavenging Assaymentioning

confidence: 99%

“…The antioxidant activity of NL13 was determined using intracellular fluorescent probe assay [17,26] on human leucocytes. Hydrogen peroxide was added as a radical generator and the ability of NL13 to remove the generated oxidative stress was measured using multimode microplate reader (Thermo Scientific) with excitation λ = 498 nm and emission λ = 530 nm.…”

Section: Analysis Of Intracellular Ros Level On Leucocytesmentioning

confidence: 99%

“…Several antioxidant compounds have been reported with wound healing activity. In the line, peptides derived from hydrolysate of A. platensis has also exhibited antioxidant activity by inhibiting lipid peroxidation formation and by donating hydrogen to act as free radical scavengers [17]. Therefore, this study focuses on the sulphur stressed antioxidant peptide and its activities including on fibroblast cells.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant molecular mechanism of adenosyl homocysteinase from cyanobacteria and its wound healing process in fibroblast cells

et al. 2020

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An antioxidant molecule namely, adenosyl homocysteinase (AHc) was identified from the earlier constructed transcriptome database of Spirulina, where it was cultured in a sulphur deprived condition. From the AHc protein, a small peptide NL13 was identified using bioinformatics tools and was predicted to have antioxidant property. Further, the peptide was synthesised and its antioxidant mechanism was addressed at molecular level. NL13 was subjected to various antioxidant assays including DPPH assay, HARS assay, SARS Assay, NO assay and ABTS assay, where NL13 exhibited significant (P < 0.05) potential antioxidant activity compared to its antioxidant control, Trolox. Cytotoxicity was performed on Human whole blood and the cell viability was performed on VERO fibroblast cells. In both assays, it was found that NL13 did not exhibit any cytotoxic effect towards the cells. Further, the intracellular ROS was performed on Multimode reader followed by imaging on fluorescence microscope which showed scavenging activity even at lower concentration of NL13 (31.2 µM). An effective wound healing property of NL13 on VERO cells was confirmed by analysing the cell migration rate at two different time intervals (24 and 48 h). Overall, the study shows that NL13 peptide scavenges the intracellular oxidative stress. Keywords Cyanobacteria • Adenosyl homocysteinase • Wound healing • Fibroblast cells • Spirulina Abbreviations AHc Adenosyl homocysteinase ROS Reactive oxygen species Ap Arthrospira platensis TEAE Trolox equivalent antioxidant capacity HRS Hydroxyl radical scavenging VERO Fibroblast cells Electronic supplementary material The online version of this article (

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Section: Validation Of Apahc Gene Expression By Real Time Pcrmentioning

confidence: 99%

Section: Abts Assaymentioning

confidence: 99%

Section: Superoxide Anion Radical Scavenging Assaymentioning

confidence: 99%

Section: Analysis Of Intracellular Ros Level On Leucocytesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antioxidant molecular mechanism of adenosyl homocysteinase from cyanobacteria and its wound healing process in fibroblast cells

et al. 2020

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Comparison of vertebrate skin structure at class level: A review

Akat

Yenmiş

Pombal

et al. 2022

The Anatomical Record

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The skin is a barrier between the internal and external environment of an organism. Depending on the species, it participates in multiple functions. The skin is the organ that holds the body together, covers and protects it, and provides communication with its environment. It is also the body's primary line of defense, especially for anamniotes. All vertebrates have multilayered skin composed of three main layers: the epidermis, the dermis, and the hypodermis. The vital mission of the integument in aquatic vertebrates is mucus secretion. Cornification began in apmhibians, improved in reptilians, and endured in avian and mammalian epidermis. The feather, the most ostentatious and functional structure of avian skin, evolved in the Mesozoic period. After the extinction of the dinosaurs, birds continued to diversify, followed by the enlargement, expansion, and diversification of mammals, which brings us to the most complicated skin organization of mammals with differing glands, cells, physiological pathways, and the evolution of hair. Throughout these radical changes, some features were preserved among classes such as basic dermal structure, pigment cell types, basic coloration genetics, and similar sensory features, which enable us to track the evolutionary path. The structural and physiological properties of the skin in all classes of vertebrates are presented. The purpose of this review is to go all the way back to the agnathans and follow the path step by step up to mammals to provide a comparative large and updated survey about vertebrate skin in terms of morphology, physiology, genetics, ecology, and immunology.

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6‐Gingerol and Semisynthetic 6‐Gingerdione Counteract Oxidative Stress Induced by ROS in Zebrafish

Manjunathan

Guru

Arokiaraj

et al. 2021

Chemistry & Biodiversity

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6-Gingerol (1) is one of the major components in ginger and developing new synthetic methodologies could bring semisynthetic analogs with improved therapeutic properties. Towards this, multigram scale isolation of 6gingerol with excellent purity was optimized using a simple and robust extraction, followed by column purification. Synthesis of 6-gingerdione, 7 from 6-gingerol was then achieved through selective -OTBDMS protection, DMP oxidation and deprotection reaction sequence for the first time. Compounds 1, 7 and 8 (dehydrozingerone) exhibited excellent cell-free antioxidant properties in DPPH, ABTS, superoxide radical scavenging assay and H 2 O 2 assay at 10-50 μM concentrations. The hemolytic study suggests that up to 50 μM, all three compounds did not exhibit toxicity to human erythrocytes. When H 2 O 2 treated zebrafish larvae groups (96hpf) were exposed to compounds 1, 7 and 8, it increases the SOD (19, 19.1 and 18.7 U/mg protein), CAT (18.1, 16.5,and 15.8 μmol/mg levels and decreases the lipid peroxidation level (13, 15 and 18 nmol/mg protein), respectively. In vivo ROS levels and degree of cell death were studied using DCFDA and Acridine orange assays. Compounds 1, 7 and 8 decreases the ROS and cell death level significantly. Taken together, compounds 1, 7 and 8 exhibit excellent antioxidant properties, counteract H 2 O 2 induced oxidative stress, reduces cell death in zebrafish larvae.

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Design and characterization of a novel Arthrospira platensis glutathione oxido-reductase-derived antioxidant peptide GM15 and its potent anti-cancer activity via caspase-9 mediated apoptosis in oral cancer cells

Cited by 53 publications

References 55 publications

Antioxidant molecular mechanism of adenosyl homocysteinase from cyanobacteria and its wound healing process in fibroblast cells

Antioxidant molecular mechanism of adenosyl homocysteinase from cyanobacteria and its wound healing process in fibroblast cells

Comparison of vertebrate skin structure at class level: A review

6‐Gingerol and Semisynthetic 6‐Gingerdione Counteract Oxidative Stress Induced by ROS in Zebrafish

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