Design and characterization of a lactate biosensor based on immobilized lactate oxidase onto gold surfaces

Parra, Ana Matus de la; Casero, Elena; Vázquez, Luís; Pariente, F.; Lorenzo, Encarnación

doi:10.1016/j.aca.2005.09.025

Cited by 129 publications

(99 citation statements)

References 23 publications

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“…Ïîñò³éíèé, îäíî÷àñíèé òà âèá³ðêîâèé ìîí³òîðèíã ëàêòàòó º íåîáõ³äíèì, òîìó ùî öåé ïàðàìåòð âèçíà÷àº íå ò³ëüêè ÿê³ñòü ³ îñîáëèâèé àðîìàò âèíà, àëå òàêîae º ³íäèêàòîðîì áàêòåð³àëüíî¿ àêòèâíîñò³ ï³ä ÷àñ ôåðìåíòàö³¿ [3]. Òîìó äîñòîâ³ðíà ³íôîð-ìàö³ÿ ïðî âì³ñò ëàêòàòó ó ñóñë³ íà ð³çíèõ ñòàä³ÿõ âèðîáíèöòâà âèíà äîçâîëÿº êîíòðîëþâàòè òà ðåãóëþâàòè ïðîöåñ áðîä³ííÿ [4]. Êð³ì òîãî, â³ä êîíöåíòðàö³¿ ëàêòàòó çàëåaeèòü ñòàá³ëüí³ñòü âèí ïðè çáåð³ãàíí³ [5].…”

Section: âñòóïunclassified

“…Íàé÷àñò³øå äëÿ ðîçðîáêè ëàêòàòíèõ àìïå-ðîìåòðè÷íèõ á³îñåíñîð³â, ïðèçíà÷åíèõ äëÿ àíàë³ç³â âèíà, âèêîðèñòîâóþòüñÿ òðè ôåðìåí-òè -ëàêòàòäåã³äðîãåíàçà (ËÄ) [3,6,8,9], ëàê-òàòîêñèäàçà (ËOÄ) [4,5,7,10,11] àáî ôëàâîöè-òîõðîì b 2 (ÔÖ b 2 ) [12].…”

Section: âñòóïunclassified

“…Çàñòîñóâàííÿ ðîçðîáëåíèõ ëàêòàòíèõ á³îñåí-ñîð³â äîçâîëÿº øâèäêî ³ áåç çíà÷íèõ çóñèëü îòðè-ìàòè äîñòîâ³ðí³ äàí³ ùîäî âì³ñòó ëàêòàòó ó âèí³, ÿê³ â³äïîâ³äàþòü ðåçóëüòàòàì êëàñè÷íèõ ìåòîä³â àíàë³çó -ñïåêòðîôîòîìåòðè÷íîãî [4,5], ôåðìå-íòàòèâíîãî [12] òà õðîìàòîãðàô³÷íîãî [6,11].…”

Section: âñòóïunclassified

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Intact Recombinant Cells of the Yeast Hansenula Polymorpha, Over-Producing Formaldehyde Dehydrogenase, as the Sensitive Bioelements for Amperometric Assay of Formaldehyde

Paryzhak

Demkiv

Schuhmann³

et al. 2008

SEMST

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Section: âñòóïunclassified

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Intact Recombinant Cells of the Yeast Hansenula Polymorpha, Over-Producing Formaldehyde Dehydrogenase, as the Sensitive Bioelements for Amperometric Assay of Formaldehyde

Paryzhak

Demkiv

Schuhmann³

et al. 2008

SEMST

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“…This value is comparable to the one reported in literature based on immobilization of lactate dehydrogenase and toluidine blue O by using a dialysis membrane and better than other two lactate biosensors where lactate oxidase was used as enzyme. 24,25 The analytical frequency was estimated to be 200 samples h -1 . The variability of measurements (inter-assay reproducibility) performed with three different biosensors was found to be 9%, demonstrating that differences in operator behavior to fabricate the biosensor are no relevant.…”

Section: Optimization Of the Biosensor Response And Analytical Paramementioning

confidence: 99%

A biosensor based on immobilization of lactate oxidase in a PB-CTAB film for FIA determination of lactate in beer samples

Lowinsohn

Bertotti

2008

J. Braz. Chem. Soc.

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No presente trabalho são apresentados resultados referentes à fabricação de um biossensor para análise de lactato. O dispositivo foi preparado imobilizando-se a enzima lactato oxidase em filme de Azul da Prússia (PB, sigla em inglês) depositado na superfície de eletrodo de carbono vítreo em soluções de CTAB. Nestas condições experimentais observou-se melhoria na sensibilidade e seletividade das determinações de lactato, as quais foram conduzidas em sistema FIA com detecção amperométrica. Nas condições otimizadas (pH = 6,9 e E = −0,1 V), obteve-se curva analítica com limite superior igual a 0,28 mmol L -1 e um limite de detecção de 0,84 µmol L -1 . A repetibilidade do método foi avaliada por meio de injeções consecutivas de amostras de lactato 0,28 mmol L -1 , obtendo-se desvio padrão de 2,2 % (n = 18). O método foi aplicado em amostras de cerveja e houve boa concordância entre os resultados obtidos e aqueles oriundos do uso de procedimento espectrofotométrico de referência.An amperometric lactate biosensor with lactate oxidase immobilized into a Prussian Blue (PB) modified electrode was fabricated. The advantage of using cetyltrimethylammonium bromide (CTAB) in the electrodeposition step of PB films onto glassy carbon surfaces was confirmed taking into account both the stability and sensitivity of the measurements. The biosensor was used in the development of a FIA amperometric method for the determination of lactate. Under optimal operating conditions (pH = 6.9, E = −0.1 V), the linear response of the method was extended up to 0.28 mmol L -1 lactate with a limit of detection of 0.84 µmol L -1 . The repeatability of the method for injections of a 0.28 mmol L -1 lactate solution was 2.2 % (n = 18). The usefulness of the method was demonstrated by determining lactate in beer samples and the results were in good agreement with those obtained by using a reference spectrophotometric enzyme method.Keywords: lactate, biosensor, prussian blue modified electrodes, CTAB, FIA amperometric detection, beer IntroductionThe relevance of metal hexacyanoferrates for electroanalytical chemistry was firstly demonstrated in the pioneering studies of Itaya et al. 1,2 involving the electrocatalysis of oxygen and hydrogen peroxide reduction at surfaces covered by Prussian Blue (PB, iron(III) hexacyanoferrate(II)) films. The transfer of electrons and the movement of small hydrated counter-cations through the channels and holes of the solid for charge compensation is a typical feature of this kind of compound. Owing to the zeolite nature of metal hexacyanoferrates, their ability as effective three-dimensional electrocatalysts has been extensively recognized.Once sufficient negative potentials are applied to PB modified electrodes, electrons are injected into the film and the so-called Prussian-White film is generated which arises from the reduction of a high-spin Fe 3+ . The reduced form (Fe 2+ ) is catalytically active towards the electron-transfer step involving hydrogen peroxide, water being formed as a final product. At positive pot...

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“…Most of the enzyme sensors proposed are based on the amperometric [9][10][11][12][13] and chemiluminometric 14 detections of hydrogen peroxide or NADH produced by the lactate oxidase or lactate dehydrogenases (LDH) as a recognition element. A new method 15 that used natural animal tissue porcine kidney as a recognition element is also proposed for the chemiluminescence sensing of L-lactic acid.…”

mentioning

confidence: 99%

Optically Specific Detection of D- and L-Lactic Acids by a Flow-Injection Dual Biosensor System with On-line Microdialysis Sampling

Nanjo

Yano

Hayashi³

et al. 2006

ANAL. SCI.

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A flow-injection dual biosensor system with microdialysis sampling is proposed for the simultaneous determination of Dlactic and L-lactic acids. The dialysate from the microdialysis tube is delivered to a sample loop of the six-way autoinjector and then automatically injected into the flow-injection line with a dual enzyme electrode arranged in perpendicular to the flow direction. The dual enzyme electrode is constructed by hybridizing a poly(1,2-diaminobenzene) film into two sensing parts which respond selectively to D-lactic and L-lactic acids, respectively, without any crossreactivity. The proposed flow-injection analysis method can be successfully applied to the simultaneous determination of D,L-lactic acids in alcoholic beverages.

show abstract

Design and characterization of a lactate biosensor based on immobilized lactate oxidase onto gold surfaces

Cited by 129 publications

References 23 publications

Intact Recombinant Cells of the Yeast Hansenula Polymorpha, Over-Producing Formaldehyde Dehydrogenase, as the Sensitive Bioelements for Amperometric Assay of Formaldehyde

Intact Recombinant Cells of the Yeast Hansenula Polymorpha, Over-Producing Formaldehyde Dehydrogenase, as the Sensitive Bioelements for Amperometric Assay of Formaldehyde

A biosensor based on immobilization of lactate oxidase in a PB-CTAB film for FIA determination of lactate in beer samples

Optically Specific Detection of D- and L-Lactic Acids by a Flow-Injection Dual Biosensor System with On-line Microdialysis Sampling

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