Design and simulation of a microfluidic device for acoustic cell separation

Shamloo, Amir; Boodaghi, Miad

doi:10.1016/j.ultras.2017.11.009

Cited by 63 publications

(38 citation statements)

References 36 publications

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“…These studies, which were carried out on the construct, reveals its potential for diagnostic application. In future studies, we aim to enhance the performance of platform by experimental implementation of ideas presented in this study and our previous studies [ 19 , 20 , 21 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

Design and Parameter Study of Integrated Microfluidic Platform for CTC Isolation and Enquiry; A Numerical Approach

2018

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Being the second cause of mortality across the globe, there is now a persistent effort to establish new cancer medication and therapies. Any accomplishment in treating cancers entails the existence of accurate identification systems empowering the early diagnosis. Recent studies indicate CTCs’ potential in cancer prognosis as well as therapy monitoring. The chief shortcoming with CTCs is that they are exceedingly rare cells in their clinically relevant concentration. Here, we simulated a microfluidic construct devised for immunomagnetic separation of the particles of interest from the background cells. This separation unit is integrated with a mixer subunit. The mixer is envisioned for mixing the CTC enriched stream with lysis buffer to extract the biological material of the cell. Some modification was proposed on mixing geometry improving the efficacy of the functional unit. A valuation of engaged forces was made and some forces were neglected due to their order of magnitude. The position of the magnet was also optimized by doing parametric study. For the mixer unit, the effect of applied voltage and frequency on mixing index was studied to find the optimal voltage and frequency which provides better mixing. Above-mentioned studies were done on isolated units and the effect of each functional unit on the other is not studied. As the final step, an integrated microfluidic platform composed of both functional subunits was simulated simultaneously. To ensure the independence of results from the grid, grid studies were also performed. The studies carried out on the construct reveal its potential for diagnostic application.

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Section: Discussionmentioning

confidence: 99%

Design and Parameter Study of Integrated Microfluidic Platform for CTC Isolation and Enquiry; A Numerical Approach

2018

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“…Parallel to experimental studies of SSAW-based separation, several numerical studies have been proposed for improving separation efficiency. Recently, Shamloo et al presented a numerical method of SSAW-based separation of platelets, red blood cells, and white blood cells [ 82 ]. In this case, to find the maximum separation efficiency, they studied the influence of applied voltage to the performance of separation.…”

Section: Saw-based Separationmentioning

confidence: 99%

Acoustic Microfluidic Separation Techniques and Bioapplications: A Review

Gao

Lin

et al. 2020

Micromachines

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Microfluidic separation technology has garnered significant attention over the past decade where particles are being separated at a micro/nanoscale in a rapid, low-cost, and simple manner. Amongst a myriad of separation technologies that have emerged thus far, acoustic microfluidic separation techniques are extremely apt to applications involving biological samples attributed to various advantages, including high controllability, biocompatibility, and non-invasive, label-free features. With that being said, downsides such as low throughput and dependence on external equipment still impede successful commercialization from laboratory-based prototypes. Here, we present a comprehensive review of recent advances in acoustic microfluidic separation techniques, along with exemplary applications. Specifically, an inclusive overview of fundamental theory and background is presented, then two sets of mechanisms underlying acoustic separation, bulk acoustic wave and surface acoustic wave, are introduced and discussed. Upon these summaries, we present a variety of applications based on acoustic separation. The primary focus is given to those associated with biological samples such as blood cells, cancer cells, proteins, bacteria, viruses, and DNA/RNA. Finally, we highlight the benefits and challenges behind burgeoning developments in the field and discuss the future perspectives and an outlook towards robust, integrated, and commercialized devices based on acoustic microfluidic separation.

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“…The principle of separation in microfluidic systems operates via well-designed microchannels and precisely controlled external physical separation methods that utilize acoustic fields [ 11 , 12 ], fluorescence activation [ 13 , 14 ], optical filing [ 15 , 16 ], magnetic activation [ 17 , 18 ], and electrical fields [ 19 , 20 , 21 ]. Shamloo et al [ 11 ] simulated the separation of blood cells with acoustic fields, while evaluating the most efficient and low-cost separation devices by altering factors of acoustic field devices. In addition, Rosental et al [ 14 ] demonstrated and developed a method to isolate different cell populations in corals and other cnidarians using fluorescent activated cell sorting (FACS) technology, which is an advanced cell sorting technique.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoresis Separation of Platelets Using a Novel Zigzag Microchannel

et al. 2020

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Platelet separation and purification are required in many applications including in the detection and treatment of hemorrhagic and thrombotic diseases, in addition to transfusions and in medical research. In this study, platelet separation was evaluated using a novel zigzag microchannel fluidic device while leveraging a dielectrophoresis (DEP) electric field using the COMSOL multiphysics software package and additional experimentation. The zigzag-shaped microchannel was superior to straight channel devices for cell separation because the sharp corners reduced the required horizontal distance needed for separation and also contributed to an asymmetric DEP electric field. A perfect linear relationship was observed between the separation distance and the corner angles. A quadratic relationship (R2 = 0.99) was observed between the driving voltage and the width and the lengths of the channel, allowing for optimization of these properties. In addition, the voltage was inversely proportional to the channel width and proportional to the channel length. An optimal velocity ratio of 1:4 was identified for the velocities of the two device inlets. The proposed device was fabricated using laser engraving and lithography with optimized structures including a 0.5 mm channel width, a 120° corner angle, a 0.3 mm channel depth, and a 17 mm channel length. A separation efficiency of 99.4% was achieved using a voltage of 20 V and a velocity ratio of 1:4. The easy fabrication, lower required voltage, label-free detection, high efficiency, and environmental friendliness of this device make it suitable for point-of-care medicine and biological applications. Moreover, it can be used for the separation of other types of compounds including lipids.

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Design and simulation of a microfluidic device for acoustic cell separation

Cited by 63 publications

References 36 publications

Design and Parameter Study of Integrated Microfluidic Platform for CTC Isolation and Enquiry; A Numerical Approach

Design and Parameter Study of Integrated Microfluidic Platform for CTC Isolation and Enquiry; A Numerical Approach

Acoustic Microfluidic Separation Techniques and Bioapplications: A Review

Dielectrophoresis Separation of Platelets Using a Novel Zigzag Microchannel

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