LES (Large-Eddy Simulation) computations were preformed to investigate the mechanisms of a kind of spanwise groove for the passive control of laminar separation bubble on the suction surface of a low-speed highly loaded low-pressure turbine blade at Re = 50,000 (Reynolds number, based on inlet velocity and axial chord length).Compared with the smooth suction surface, the numerical results indicate that: (1) the groove is effective to shorten and thin the separation bubble, which contributes the flow loss reduction on the groove surface, by thinning the boundary layer behind the groove and promoting earlier transition inception in the separation bubble; (2) upstream movement of the transition inception location on the grooved surface is suggested being the result of the lower frequency at which the highest amplification rate of instability waves occurs, and the larger initial amplitude of the disturbance at the most unstable frequency before transition; and (3) the viscous instability mode is promoted on the grooved surface, due to the thinning of the boundary layer behind the groove.