Developments in neutron activation analysis in its various modes for trace element determination are described with reference to reactor and other neutron sources, competing methods, tomography and detection systems. A selected number of areas of application are highlighted which provide challenges into the next millennium and to which a useful and in some cases a unique contribution can be made. The role that neutron activation analysis can play in decommissioning, landmine detection, boron neutron capture therapy, Alzheimer's disease, diabetes mellitus, the complex mechanisms of initiation and termination of feeding and obesity as well as in scar formation and the requirements for artificial skin are presented.