Design of an imprinted clean-up method for mycophenolic acid in maize

Smet, David De; Kodeck, Valérie; Dubruel, Peter; Peteghem, Carlos Van; Schacht, Etienne; Saeger, Sarah De

doi:10.1016/j.chroma.2010.12.085

Cited by 11 publications

(9 citation statements)

References 31 publications

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“…In fact, the imprinting factor for OTA is IF=6.83 measured onto the polymer imprinted with CHNA-Phe, IF=8.00 for CHNA-Ala and IF=7.71 for CHNA-Gly-imprinted polymers. This data shows that not only is a bulky amino acid side chain not essential for an efficient mimic template of OTA, but that a template is able to induce the formation of an imprinted polymer with binding properties towards a slightly larger target molecule, clearly confirming what had been previously reported in literature for polymers imprinted with carbamates [16] or mimics of micotoxins other than OTA, such as citrinin [4], mycophenolic acid [5], and domoic acid [17].…”

Section: Type-1 Mimic Templatessupporting

confidence: 77%

“…When the analogue is difficult to synthesize, expensive, or simply not available as a commercial product, it could be convenient to use commercially available substances that are less strictly related to the target analyte, but having a more limited molecular recognition effect [2][3][4][5]. In this case, in fact, the structural differences between the analyte and the mimic template become significant, and the similarity between molecules remains confined to the overall molecular shape and the preservation of substituents able to form non-covalent interactions with the binding sites.…”

Section: Introductionmentioning

confidence: 99%

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Effect of the mimic structure on the molecular recognition properties of molecularly imprinted polymers for ochratoxin A prepared by a fragmental approach

Baggiani¹,

Biagioli²,

Anfossi³

et al. 2013

Reactive and Functional Polymers

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In this work the fragmental approach was used to prepare several molecularly imprinted ethylene dimethacrylate-co-methacrylic acid polymers with molecular recognition towards the mycotoxin ochratoxin A, with the aim of searching for simpler mimic templates than the well-known N-(4-chloro-1-hydroxy-2-naphthoylamido)-(L)-phenylalanine. The screening for binding of two different kinds of ochratoxin-related molecules was performed by HPLC analysis. Ochratoxin A and the mimic templates were eluted in acetonitrile -acetic acid (0.1% v/v) and the imprinting factor was measured for all the ligands on all the columns packed with the imprinted polymers. The experimental results show that changes to the amino acidic sub-structure or the presence/absence of a chlorine atom in position 4 on the naphtalene ring system does not affect the molecular recognition of ochratoxin A by the resulting imprinted polymer. On the contrary, the presence of the bulky naphtalene ring system in the mimic template seems to be necessary to preserve the molecular recognition of ochratoxin. This binding behavior was found to be compatible with in silico simulations of the complexation between some of the mimic templates and molecules of methacrylic acid. The use of the mimic template N-(1-hydroxy-2-naphthoylamido)-(L)-phenylalanine seems to represent a synthetically simple approach to the preparation of imprinted polymers with molecular recognition properties towards ochratoxin A.

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Section: Type-1 Mimic Templatessupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Effect of the mimic structure on the molecular recognition properties of molecularly imprinted polymers for ochratoxin A prepared by a fragmental approach

Baggiani¹,

Biagioli²,

Anfossi³

et al. 2013

Reactive and Functional Polymers

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“…Due to the low acute toxicity of MPA, the relevance of this compound as a mycotoxin has been questioned but high toxin concentrations, found often in maize silage heavily infested with Penicillium roqueforti, raised concerns about adverse long-term effects of this mycotoxin in livestock (De Smet et al 2011;Schneweis et al 2000). However, at least in white blood cells of sheep fed with extreme high doses of MPA, no significant influence on immunoglobulins and expression of cytokines were found (Dzidic et al 2010).…”

Section: Introductionmentioning

confidence: 95%

“…Particularly in recent years, MPA analyses of food and feed were often conducted as part of multi-analyte approaches (Pizzutti et al 2014;Sulyok et al 2010;Vaclavik et al 2013;Van Pamel et al 2011;Zachariasova et al 2014), but as stated by De Smet et al (2011), most existing multianalytical methods lack sensitivity due to the absence of proper clean-up methods. As an alternative approach, sensitive immunochemical methods based on polyclonal rabbit antisera have been described by Usleber et al (2008) and Burkin and Kononenko (2010).…”

Section: Introductionmentioning

confidence: 99%

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Development and application of monoclonal antibodies against the mycotoxin mycophenolic acid

Dietrich

Märtlbauer

2015

Mycotoxin Res

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Mycophenolic acid (MPA) is frequently found, often in high concentrations, in a broad range of food and feed matrices. Apart from the well-known contamination of blue-veined cheeses caused by the use of toxinogenic Penicillium roqueforti strains for manufacturing, a broad range of other Penicillium spp. is able to produce this immunosuppressive toxin. Therefore, MPA has been proposed to be a suitable marker for Penicillium-infected food commodities. In the present work, a high-affinity monoclonal antibody (mAb) for the specific detection of MPA was developed by immunizing mice with a MPA-protein conjugate coupled by an activated ester method. Under the conditions of a direct competitive enzyme immunoassay (EIA), 50% inhibition and detection limits of MPA standard curves were 1.2 and 0.3 ng/ml, respectively. Furthermore, the mAb could be successfully employed for the production of an immunoaffinity (IA) column enabling the efficient enrichment of MPA from processed foodstuffs. By combining the IA clean-up with a polyclonal antibody-based EIA, an ultrasensitive analysis method could be established which allowed the reliable and reproducible detection of MPA in artificially contaminated tomato ketchup as a model matrix at concentrations as low as 0.1 ng/g.

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Molecular imprinting science and technology: a survey of the literature for the years 2004-2011

2014

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SummaryHerein we present a survey of the literature covering the development of molecular imprinting science and technology over the years 2004 to 2011. In total, 3779 references to the original papers, reviews, edited volumes and monographs from this period are included, along with recently identified uncited materials from prior to 2004 which were omitted in the first instalment of this series covering the years 1930 to 2003. 1 In the presentation of the assembled references, a section presenting reviews and monographs covering the area is followed by sections describing fundamental aspects of molecular imprinting including the development of novel polymer formats. Thereafter, literature describing efforts to apply these polymeric materials to a range of application areas is presented. Current trends and areas of rapid development are discussed.2

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Design of an imprinted clean-up method for mycophenolic acid in maize

Cited by 11 publications

References 31 publications

Effect of the mimic structure on the molecular recognition properties of molecularly imprinted polymers for ochratoxin A prepared by a fragmental approach

Effect of the mimic structure on the molecular recognition properties of molecularly imprinted polymers for ochratoxin A prepared by a fragmental approach

Development and application of monoclonal antibodies against the mycotoxin mycophenolic acid

Molecular imprinting science and technology: a survey of the literature for the years 2004-2011

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