Design of dendrimer modified carbon nanotubes for gene delivery

Effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes on murine embryonicstem cells

“…Considering oxidative stress as a dominant mechanism underlying the experimentally observed ES cell cytotoxicity after exposure and internalization of high doses of FMNTs, we could speculate that low doses of FMNTs generate a few reactive oxygen species acting as regulators of cell differentiation. Molecular evidence for such a hypothesis was recently reported by Spike and Macleod [27][28][29][30][31][32], who identified the pRb tumor suppressor pathway as a critical cell cycle regulator of hematopoietic hemostasis at the G2/M checkpoint under oxidative stress. Future studies are required to define the molecular consequences Figure 4 Effect of FMNTs on the differentiation of embryonic stem cells.…”

“…In contrast, Pantorotto et al hypothesized that the nanotube uptake by living cells can also occur via an energy-independent nanoendocytosis pathway that involves insertion and diffusion of nanotubes across cell membranes [20,21]. Stem cells membranes, however, exhibit a significantly different glycoprotein composition than more mature hematopoietic cells [32]. Consequently, internalization mechanisms underlying cellular accumulation of FMNTs in CCE ES cells may differ from those established in tumor cells.…”

Dendrimer Modified SWCNTs for High Efficient Delivery and Intracellular Imaging of Survivin siRNA

“…For example, carbon nanotubes have been used as delivery system for gene therapy [4], also used for biosensor for ultrasensitive detection of biomarkers [5], more important, carbon nanotubes can be used for near infrared imaging and photothermal therapy [6]. In our previous work, we also confirmed that carbon nanotubes can be filled with DNA or peptide molecules [7,8], can take antisense oligonucleotides into HL-60 cells and inhibited tumor cell growth [9], highly suggesting that carbon nanotubes may be good delivery system and have highly potential in gene or drug storage and delivery system in molecular therapy of diseases.…”

Dendrimer-Functionalized Multi-walled Carbon Nanotubes Exhibit Dual-Phase Regulation to Exposed Murine Embryonic Stem Cells

“…Dendrimers are a novel special class of organic molecules: they can take different functional groups through a series of chemical modifications, and their interior cavities can serve as storage areas for a lot of genes or drugs [15,16]. Dendrimers may be a good nonviral delivery vector because it has the advantages of safety, simplicity of use, and ease of mass production compared with viral vectors that are inherently risky [17,18].…”