Design of portable electrochemiluminescence sensing systems for point-of-care-testing applications

Electrochemiluminescence devices for point-of-care testing

“…1). 11,16,34 Both LFS and μPADs possess the ability of separating complex samples through the capillary action of cellulose materials, but LFS has a specific structure for sample addition, target recognition and capture. ECL cells can emit light steadily for a long time, thus avoiding the instability caused by solution evaporation.…”

“…[5][6][7][8][9][10] As reported recently, the global POCT market was about 35.5 billion dollars in 2021 and is expected to exceed 96.0 billion dollars in 2025. 11 POCT devices can report the detection results by different methods, such as electrochemistry (EC), high-performance liquid chromatography (HPLC), electrochemiluminescence (ECL), fluorescence (FL), etc. [12][13][14][15] Among them, ECL is an ideal one for POCT, because of its advantages of high sensitivity, low background, no requirement of an external light source for excitation and high spatiotemporal controllability.…”

“…5–10 As reported recently, the global POCT market was about 35.5 billion dollars in 2021 and is expected to exceed 96.0 billion dollars in 2025. 11…”

Imaging and Simulation of Ruthenium Derivative Coating Microbeads at the Opaque Electrode with Electrogenerated Chemiluminescence

“…Electrogenerated chemiluminescence (electrochemiluminescence, ECL) is a kind of luminescence that occurs at or near the surface of the electrode generated by electron transfer reactions between electrochemically produced intermediates. − The ECL imaging method, as a promising imaging method by recording ECL emissions on a charge coupled device paired with a microscope, is gaining increasing interest in recent years because of its high sensitivity, low background noise, and good temporal and spatial resolution. ECL imaging has been used in visualizing electrode, − microbeads, − nanomaterials, , entities, − latent fingerprint, , as well as qualifying and imaging proteins on the cell surface, − small molecules in the cell membrane, , or biological molecules released from cells , and cell morphology, as well as food safety inspection and environment surveillance . For example, our group developed an ECL imaging method for the determination of cell surface protein at the gold electrode and for visualizing cell morphology at the glassy carbon electrode (GCE) .…”

Vesicles as a Multifunctional Microenvironment for Electrochemiluminescence Signal Amplification

“…Electrochemiluminescence (ECL) is a luminescent phenomenon that arises from the electron transfer reaction of species generated on the electrode surface. − The microenvironment of the electrode surface is crucial to modulate the ECL signals because the electrode surface can affect the electron transfer kinetics of ECL reactions, the adsorption and desorption of ECL reactants, and the stability of ECL intermediates. − To achieve the amplified ECL signals, a suitable reaction microenvironment on the electrode surface is one of the primary strategies . For example, conductive microenvironments are able to promote the electron transfer efficiency of ECL reactions; − porous microenvironments can facilitate the adsorption and mass transfer of ECL reactants; − and hydrophobic microenvironments are capable of improving the stability of ECL luminophores and reaction intermediates. , However, the construction of multifunctional microenvironments as a versatile amplification platform for ECL systems with different reaction mechanisms has rarely been explored due to the difficulty of integrating various functional components into a single microstructure.…”