Design optimization and characterization of a small-scale centrifugal cell separator

Leatzow, Dan M.; Wie, Bernard J. Van; Weyrauch, Bruce; Tiffany, T. O.

doi:10.1016/s0003-2670(01)00863-7

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…The deeper region allows more milk sample to be contained in a given device area, while the shallower region creates a larger total area of compacted cells for quantitative measurement. Because the centrifugal acceleration can be separated into normal and tangential components at the sloping "floor" (Leatzow et al 2001), the low 5.5°angle keeps the tangential component comparatively small, decreasing the likelihood that cells will become trapped along the sloping floor as they move into the closed-end channel. The V-shaped region of the funnel has a maximum angle of 37°relative to the disc radius, (Walstra et al 1999) possibly larger than ideal for minimizing cell trapping along this region of the side walls, but the sample volume, height of the disc, and radius of the disc constrained this dimensional choice, which proved to be acceptable.…”

Section: Sample Volume and Channel Geometry And Dimensionsmentioning

confidence: 99%

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Microfluidic sedimentation cytometer for milk quality and bovine mastitis monitoring

García-Cordero

Barrett

O’Kennedy

et al. 2010

Biomed Microdevices

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We report a rapid, low-cost, portable microfluidic sedimentation cytometer (SeCy) for assessing the somatic cell count and fat content of milk in 15 min using a "sample-in, answer-out" approach. The system consists of 12 independent microfluidic devices, essentially flattened funnel structures, fabricated on the footprint of a single plastic compact disc (CD). Each funnel structure holds 150 μL of milk, has an inlet for milk filling and an outlet for air to escape, and ends in a narrow, closed-end microfluidic channel that facilitates packing of the cells into a column whose length is proportional to cell count. The closed-end channel provides accurate cell counts over the range 50,000->3,000,000 cells per mL. The assay separates cells and fat globules based on their densities (by differential sedimentation), concentrating white cells in the closed-end channel near the outer rim of the CD for estimation of total "cell pellet" volume, while fat globules move toward the center of disc rotation, forming a fat "band" in the funnel. After adding milk to two or more microfluidic devices, the CD is loaded onto a custom-built reader unit that spins the disc for 15 min. Two low-cost microscopes in the reader image the centrifuged cell pellet and the fat band, providing a sufficiently accurate cell count to diagnose mastitis and measuring fat content as an indication of health and nutritional status.

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Section: Sample Volume and Channel Geometry And Dimensionsmentioning

confidence: 99%

“…It is also likely that some of the cells roll along the wall(s) as they move toward the closed-end channel, decreasing the average settling velocity. Leatzow et al found that small differences in the angles of tapered walls can cause as much as a 6-fold difference in the separation time (Leatzow et al 2001).…”

Section: Somatic Cell Sedimentation Timementioning

confidence: 99%

Microfluidic sedimentation cytometer for milk quality and bovine mastitis monitoring

García-Cordero

Barrett

O’Kennedy

et al. 2010

Biomed Microdevices

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“…In order to calculate the apparent porosity for each species we use the method proposed by Patwardahan and Tien ! , first applied to blood cells by Van Wie and Hustvedt 3 then by Leatzow et al 4 to calculate the average weighted particle diameter ! "# and the porosity of the fluidized bed, assuming that the highest suspended particles indicate the end of the fluidized bed.…”

Section: System Analysismentioning

confidence: 99%

Work in Progress:Development of Hands-on Desktop Learning Modules for Bioengineering Courses

Arasteh

Clark

Wie

et al.

2013 ASEE Annual Conference &Amp; Exposition Proceedings

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An experimental investigation of interfacial instability in separated blood

et al. 2019

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Experiments are done with separated human blood decelerated at constant rates to determine the maximum deceleration rate while avoiding remixing of the layers, which have different densities and viscosities. The deceleration rate affects both the stability and separation of particles through sedimentation. The velocity at onset of instability for a constant deceleration rate is experimentally determined for a 12‐cm‐diameter disk. Parameters of cell pack thickness, plasma thickness, and total thickness are investigated experimentally, and the only statistically significant parameter to affect stability is rate of deceleration. The data describe a correlation separating regions of stability and instability. To understand stability in decelerating flow of separated blood plasma and cells, the equations of motion are solved for a two‐layer fluid flow with a moving boundary and free surface. Inclusion of non‐Newtonian rheology of blood predicts large discontinuities in the shear rate, which is proposed as the cause of the interfacial instabilities. © 2019 American Institute of Chemical Engineers AIChE J, 65: 1376–1386, 2019

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Design optimization and characterization of a small-scale centrifugal cell separator

Cited by 3 publications

References 15 publications

Microfluidic sedimentation cytometer for milk quality and bovine mastitis monitoring

Microfluidic sedimentation cytometer for milk quality and bovine mastitis monitoring

Work in Progress:Development of Hands-on Desktop Learning Modules for Bioengineering Courses

An experimental investigation of interfacial instability in separated blood

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