Desinfección de segmentos nodales sobre el rendimiento morfogenético de vitroplantas de Vanilla planifolia Andrews

Bolaños, José Bernal Azofeifa; Rivera-Coto, Germán; Paniagua-Vásquez, Amelia; Cordero-Solórzano, Roberto; Salas-Alvarado, Eduardo

doi:10.15517/am.v30i1.32360

Cited by 5 publications

(3 citation statements)

References 18 publications

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“…Contamination with bacteria, fungi, yeasts, or viruses has been recognized as the most important cause of in vitro culture failure [64,65]. One of the most widely used disinfectants is NaClO [66][67][68][69], applied as a disinfection treatment in research on P. caerulea [70], P. edulis [40] and P. suberosa [31]. The treatment with NaClO solution preceded by EtOH [71] has also been used in the case of the P. miniata species [59].…”

Section: Discussionmentioning

confidence: 99%

Plant Regeneration Protocol for Recalcitrant Passionflower (Passiflora quadrangularis L.)

et al. 2022

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This research was designed to provide the first protocol to establish an efficient solution for direct organogenesis regeneration in Passiflora quadrangularis using nodal explants from young shoots. Passifloraceae tissue culture has been associated with problems such as recalcitrance, sensitivity to ethylene accumulation and browning of explants due to the presence of phenols in the tissues. Due to the high rate of endogenous contamination of the explants, a preliminary experiment was performed. The best results of surface sterilization were obtained using the pretreatment with 70% EtOH, 1 min and 50% NaOCl, 10 min along with the treatment of Rifampicin 15 µg/mL and Benomyl 2 g/L. The effects of plant growth regulators on the induction of direct organogenesis, multiplication of shoots in subcultures and in vitro rooting were evaluated. Additional compounds such as AgNO3 and Pluronic F-68 were added to the culture media in order to reduce the effects of phenols and the sudden browning of the explants. Shoot proliferation increased to the sixth subculture after which it decreased. A maximum of 7.17 shoots were obtained from one shoot on Murashige and Skoog (MS) medium supplemented with 2 mg/L 6-benzylaminopurine and 1 mg/L thidiazuron. Supplementation of ½ MS medium with 1 mg/L 1-naphthaleneacetic acid was conducing to root formation in 61.11% of shoots. After acclimatization, the plants showed vigorous growth, green leaves, and well-developed roots. Although this species has previously shown difficulty in in vitro propagation, this protocol established based on the results proved to be efficient and reproducible.

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Section: Discussionmentioning

confidence: 99%

Plant Regeneration Protocol for Recalcitrant Passionflower (Passiflora quadrangularis L.)

et al. 2022

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“…El género Vanilla está representado por un grupo de lianas que comprende 126 especies pertenecientes a la familia Orchidacea (Damian y Mitidieri 2020, Azofeifa-Bolaños et al 2019 Dressler 2010, Bory et al 2008). Según Gelski (2019).…”

Section: Introductionunclassified

Caracterización morfogénica de la vainilla (Vanilla planifolia Andrews) para su propagación

Ramírez

Zúñiga

2022

RAC

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Introducción. La vainilla (Vanilla planifolia Andrews) es una orquídea que se cultiva comercialmente para la producción de vainillina, una sustancia saborizante que se emplea principalmente en la industria alimenticia y como cosmético. Aunque la demanda ha motivado su cultivo, hay poca disponibilidad de semilla vigorosa y libre de enfermedades lo cual ha sido una limitante. Se ha demostrado que los sistemas de inmersión temporal son eficientes. Para la micropropagación masiva de la vainilla, aún no se han establecido estudios de tiempo, frecuencia de inmersión y volumen óptimo en la proliferación de brotes en Biorreactores de Inmersión Temporal (BIT) y además se desconoce una ruta morfogénica que garantice la estabilidad genética y la optimización durante el proceso de micropropagación. Objetivo. Caracterización morfogénica de la vainilla (Vanilla planifolia Andrews) en un BIT. Materiales y métodos. Se realizaron 3 experimentos, cada uno con 3 tratamientos. Para el primer experimento, se consideraron tiempos de 5, 10 y 15 min para los tratamientos, en el segundo se evaluaron 3 frecuencias en tiempos de 6, 12 y 18 horas y en el tercero se consideraron 20, 30 y 40 ml/explante como volumen de medio de cultivo. Para los diferentes tratamientos se utilizaron 5 repeticiones, con una unidad experimental de 10 explantes y segmentos de tallo con 3 yemas axilares. El medio de cultivo consistió en las sales de Murashige Skoog (MS) suplementado con 1 mg.L-1 de BA y 1 g.L-1 de caseína. El fotoperiodo se ajustó a 16 horas luz, con una temperatura de 27°C±3 y una humedad relativa de 80% Resultados. Se obtuvieron diferencias estadísticas (p<0,05) respecto a los tiempos evaluados, frecuencia de inmersión y el volumen por explante. Se determinó como mejor tiempo de inmersión un periodo de 15 minutos, 6 horas como frecuencia de inmersión y 40 ml como volumen de medio por explante. Al comparar la respuesta de desarrollo, el sistema BIT superó al medio semisólido 1,8 veces en el número de brotes y 4 veces en el incremento de peso fresco. También con BIT se obtuvieron 36 brotes por explante mientras que en el sistema de cultivo en medio semisólido, se obtuvieron 20 brotes por explante. Conclusiones. Mediante el empleo del sistema BIT, se optimizó el mecanismo de micropropagación de V. planifolia comparado con el medio de cultivo semisólido en la ruta organogénica directa, lo cual fue demostrado con la formación de brotes preformados y brotes adventicios.

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“…To initiate in vitro cultures, there is a serious problem with contaminants, as they compete advantageously with plants for the nutrients in the culture medium and end up killing the tissues (AZOFEIFA-BOLAÑOS et al, 2019). Therefore, it is necessary to develop efficient explant disinfection protocols that eliminate exogenous microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Establishment of in vitro aseptic culture of Philodendron xanadu Croat

Lara-Ascencio

Andrade-Rodríguez

Guillén-Sánchez

et al. 2021

Revista Ciência Agronômica

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The philodendron is a plant with a high sales price; however, in Mexico there is not enough plant material available for the producers of this ornamental. Therefore, the objective of this research was to establish an in vitro aseptic culture of philodendron; for this, the effect of fungicides and bactericides applied from the transplantation of mother plants to the production and harvest of explants (axillary shoots) for in vitro establishment was evaluated. Two experiments were carried out using MS (MURASHIGE; SKOOG, 1962) culture medium; in the second, silver nanoparticles (AgNPs) were added to the culture medium. Both experiments were established in a completely randomized experimental design. It was observed that with the joint action of the sanitizing products in the treatment of mother plants and in the disinfection process, as well as the addition of AgNPs to the culture medium, it was possible to establish an aseptic culture of philodendron. Results show that 2 g L -1 of Agry-Gent Plus 5000 ® plus 2 g L -1 of Prozycar ® in the treatment of mother plants was the one generated the highest percentage of aseptic explants (25.71%) in the first in vitro establishment, while, in the second experiment, the same products plus the addition of AgNPs to the culture medium for seven days generated 100% aseptic explants. The contaminants identified were yeasts, Aspergillus sp. and Penicillium sp.

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Desinfección de segmentos nodales sobre el rendimiento morfogenético de vitroplantas de Vanilla planifolia Andrews

Cited by 5 publications

References 18 publications

Plant Regeneration Protocol for Recalcitrant Passionflower (Passiflora quadrangularis L.)

Plant Regeneration Protocol for Recalcitrant Passionflower (Passiflora quadrangularis L.)

Caracterización morfogénica de la vainilla (Vanilla planifolia Andrews) para su propagación

Establishment of in vitro aseptic culture of Philodendron xanadu Croat

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