Desulfatiglans anilini Initiates Degradation of Aniline With the Production of Phenylphosphoamidate and 4-Aminobenzoate as Intermediates Through Synthases and Carboxylases From Different Gene Clusters

Xie, Xiaoman; Spiteller, Dieter; Huhn, Thomas; Schink, Bernhard; Müller, Nicolai

doi:10.3389/fmicb.2020.02064

Cited by 3 publications

(2 citation statements)

References 46 publications

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“…The adjacent clade of SEEP-SRB1c is composed of the alkane degrader Desulfococcus oleovorans (Dorries et al, 2016) and the aromatic hydrocarbon degrader Desulfobacula toluolica (Wohlbrand et al, 2013), all using sulfate as electron acceptors. The clade consisting of Syntrophobacter fumaroxidans (Plugge et al, 2012) does not have representative SRB genomes and is probably formed by SRB that are able to ferment organic molecules such as aromatic compounds, fatty acids and propanoate to produce CO 2 and H 2 , exemplified by Desulfarculus baarsii (Sun et al, 2010) and Desulfatiglans anilini (Xie et al, 2020) in the clade. Most SRB MAGs (n = 56; 81.16%) were not associated with a known SRB species in the phylogenomics tree.…”

Section: Identification Of Potential Sob and Srbmentioning

confidence: 99%

Microbial ecology of sulfur cycling near the sulfate–methane transition of deep‐sea cold seep sediments

Dong

et al. 2021

Environmental Microbiology

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Microbial sulfate reduction is largely associated with anaerobic methane oxidation and alkane degradation in sulfate-methane transition zone (SMTZ) of deepsea cold seeps. How the sulfur cycling is mediated by microbes near SMTZ has not been fully understood. In this study, we detected a shallow SMTZ in three of eight sediment cores sampled from two cold seep areas in the South China Sea. One hundred ten genomes representing sulfur-oxidizing bacteria (SOB) and sulfur-reducing bacteria (SRB) strains were identified from three SMTZ-bearing cores. In the layers above SMTZ, SOB were mostly constituted by Campylobacterota, Gammaproteobacteria and Alphaproteobacteria that probably depended on nitrogen oxides and/or oxygen for oxidation of sulfide and thiosulfate in near-surface sediment layers. In the layers below the SMTZ, the deltaproteobacterial SRB genomes and metatranscriptomes revealed CO 2 fixation by Wood-Ljungdahl pathway, sulfate reduction and nitrogen fixation for syntrophic or fermentative lifestyle. A total of 68% of the metagenome assembled genomes were not adjacent to known species in a phylogenomic tree, indicating a high diversity of bacteria involved in sulfur cycling. With the large number of genomes for SOB and SRB, our study uncovers the microbial populations that potentially mediate sulfur metabolism and associated carbon and nitrogen cycles, which sheds light on complex biogeochemical processes in deep-sea environments.

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Section: Identification Of Potential Sob and Srbmentioning

confidence: 99%

Microbial ecology of sulfur cycling near the sulfate–methane transition of deep‐sea cold seep sediments

Dong

et al. 2021

Environmental Microbiology

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“…For total proteome analysis of cell-free extracts of P. fastidiosa , samples were digested with trypsin and analyzed by mass spectrometry at the Proteomics Facility of the University of Konstanz ( www.biologie.uni-konstanz.de/proteomics-centre/ ) and the peptide fingerprints matched against a local database of all predicted proteins of the JGI’s isolate genome annotation as described previously ( 49 – 51 ). Mass spectrometry was done with a LTQ Orbitrap Discovery with an Eksigent 2D-nano HPLC (Thermo Fisher Scientific, Waltham, MA, United States) or a Q-Exactive HF mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) interfaced with an Easy-nLC 1200 nanoflow liquid chromatography system (Thermo Scientific, Odense, Denmark).…”

Section: Methodsmentioning

confidence: 99%

AMP-dependent phosphite dehydrogenase, a phosphorylating enzyme in dissimilatory phosphite oxidation

Mao,

Fleming,

Mayans

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

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Oxidation of phosphite (HPO 3 2− ) to phosphate (HPO 4 2− ) releases electrons at a very low redox potential (E 0 ′ = −690 mV) which renders phosphite an excellent electron donor for microbial energy metabolism. To date, two pure cultures of strictly anaerobic bacteria have been isolated that run their energy metabolism on the basis of phosphite oxidation, the Gram-negative Desulfotignum phosphitoxidans (DSM 13687) and the Gram-positive Phosphitispora fastidiosa (DSM 112739). Here, we describe the key enzyme for dissimilatory phosphite oxidation in these bacteria. The enzyme catalyzed phosphite oxidation in the presence of adenosine monophosphate (AMP) to form adenosine diphosphate (ADP), with concomitant reduction of oxidized nicotinamide adenine dinucleotide (NAD + ) to reduced nicotinamide adenine dinucleotide (NADH). The enzyme of P. fastidiosa was heterologously expressed in Escherichia coli . It has a molecular mass of 35.2 kDa and a high affinity for phosphite and NAD + . Its activity was enhanced more than 100-fold by addition of ADP-consuming adenylate kinase (myokinase) to a maximal activity between 30 and 80 mU x mg protein −1 . A similar NAD-dependent enzyme oxidizing phosphite to phosphate with concomitant phosphorylation of AMP to ADP is found in D. phosphitoxidans, but this enzyme could not be heterologously expressed. Based on sequence analysis, these phosphite-oxidizing enzymes are related to nucleotide-diphosphate-sugar epimerases and indeed represent AMP-dependent phosphite dehydrogenases (ApdA). A reaction mechanism is proposed for this unusual type of substrate-level phosphorylation reaction.

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Degradation of Aniline and Antimony in Printing and Dyeing Wastewater by Micro-Oxygenated Hydrolytic Acidification and Their Removal Effects on Chemical Oxygen Demand and Ammonia Nitrogen

Zhang,

Ye,

Liu

2024

Water

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The degradation characteristics of aniline and antimony in printing and dyeing wastewater during the micro-oxygenated hydrolytic acidification process and its effect on COD and ammonia nitrogen removal were investigated in this experiment. Firstly, the effects of control factors such as pH, dissolved oxygen (DO), and sludge concentration on COD and ammonia nitrogen removal in the hydrolysis acidification section were optimized. It was recommended that the pH value should be maintained at 6.5; low DO (0–0.5 mg/L) could assist in the conversion of nitrogen for subsequent treatment; the optimum treatment temperature was 25 °C; finally, it was recommended that the sludge concentration should be controlled at 4 mg/L during the operation. Secondly, the effects of aniline and antimony on COD and nitrogen removal were investigated. It was found that when the concentration of aniline was increased from 0.4 mg/L to 5.4 mg/L, the COD concentration in the effluent increased by 96.5%, which indicated that aniline was toxic to anaerobic sludge and obviously inhibited the degradation of COD. When the concentration of antimony was increased from 0.05 mg/L to 2.05 mg/L, the COD removal rate was only 2.9%, which was much lower than that of the water samples with no antimony added. The anaerobic sludge concentration decreased from 5.58 g/L to 3.44 g/L, which indicated that aniline and antimony had a strong inhibitory effect on the activity of anaerobic bacteria and inversely affected COD removal.

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Desulfatiglans anilini Initiates Degradation of Aniline With the Production of Phenylphosphoamidate and 4-Aminobenzoate as Intermediates Through Synthases and Carboxylases From Different Gene Clusters

Cited by 3 publications

References 46 publications

Microbial ecology of sulfur cycling near the sulfate–methane transition of deep‐sea cold seep sediments

Microbial ecology of sulfur cycling near the sulfate–methane transition of deep‐sea cold seep sediments

AMP-dependent phosphite dehydrogenase, a phosphorylating enzyme in dissimilatory phosphite oxidation

Degradation of Aniline and Antimony in Printing and Dyeing Wastewater by Micro-Oxygenated Hydrolytic Acidification and Their Removal Effects on Chemical Oxygen Demand and Ammonia Nitrogen

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