Detecting lipid droplets polarity: Silicone-based unique fluorescent probe for cancer diagnosis in living cells

Dynamic Excimer (DYNEX) Imaging of Lipid Droplets

“…A close inspection of the droplet images (Figures B,C and S15) clearly exhibited lower polarity in the inner core of the droplets, in agreement with the aforementioned arrangement . Interestingly, compared with the CCD-18Co line, the tumoral CT26.WT cell line exhibited lipid droplets with slightly lower polarity, consistent with previous works. ,,− This observation supports the idea of a higher lipid content in the tumoral line due to altered, proliferative metabolism.…”

“…For these measurements, once lipid droplet pixels were segmented using DYNEX, we extracted polarity E T 30 values (Figure ) via multiparametric microscopy by combining the time-gated intensity ratio and fluorescence lifetime information (see the SI for experimental details and Figures S11–S13). Heretofore, there have been some efforts to characterize intracellular polarity employing fluorescence microscopy techniques using emission spectra, fluorescence intensity, and ratiometric measures. − Nevertheless, biological samples present important difficulties for quantitative imaging that are overcome by our methodology Table and Figure S14 show the E T 30 values recovered from lipid droplets of CCD-18Co and CT26.WT cells.…”

A lipid droplet-targetable and biothiol-sensitive fluorescent probe for the diagnosis of cancer cells/tissues

“…15,16 So a large number of LDs with low polarity have been reported as potential markers of cancer diagnosis and treatment. [17][18][19][20][21][22][23][24][25][26] Besides, mounting research also revealed that some cancerspecific microenvironments, including decreased extracellular pH and enhanced levels of intracellular (especially within mitochondria) glutathione (GSH), reactive oxygen species (ROS) and viscosity, could be regarded as effective tumor markers regardless of the cancer type. [27][28][29][30][31] Among them, the monitoring of biothiols (including GSH, Cys and Hcy) under physiological and pathological conditions has attracted extensive attention, and some studies could effectively distinguish these biothiols.…”

A water-soluble polymer fluorescent probeviaRAFT polymerization for dynamic monitoring of cellular lipid droplet levels and zebrafish imaging

“…The emergence and development of fluorescent probe technology have attracted the attention of a large number of researchers so far. [15][16][17][18][19][20] Compared with traditional detection methods, fluorescence sensing technology exhibits excellent timeliness and convenience. [21][22][23][24][25][26][27][28] Naturally, a vast amount of fluorescent probes for specific labeling of lipid droplets have been developed.…”