Detection and characterization of leishmania antigens from an American cutaneous leishmaniasis vaccine for diagnosis of visceral leishmaniasis

Post Challenging Serum Cytokine Profile (Th1 & Th2) in the Vaccinated Mice (Balb/C) With a New Formulation of Leishmania major Antigen

“…For most species, humans are accidental hosts since Leishmania is primarily a zoonotic disease or has recent zoonotic forms of disease in areas not considered previously to be endemic (2). Up to now, successful vaccination strategy against Leishmaniasis has been limited to cutaneous Leishmania with small doses of living virulent L. major promastigotes as a selected site (3). Although mouse models have been used for the study of both cutaneous and visceral Leishmaniasis, they more closely reflect the human cutaneous Leishmaniasis than visceral disease.…”

Identification of Proteins in Promastigote and Amastigote-like Leishmania Using an Immunoproteomic Approach

“…The diagnosis of CVL, by means of ELISA, based on Leishmania antigens has shown variable values of sensitivity and/or specificity, mainly due to antigenic similarities between Leishmania and other protozoa [10]. As a strategy to develop a more specific test, several parasite antigens have been tested in prior studies [11]–[14]; however, due to frequent low specificity and sensitivity in detecting asymptomatic infections and the high variability observed in the humoral response of individual infected dogs [15], it has been postulated that an efficient diagnosis may require a mixture of antigens or the use of chimerical antigens [16]–[19].…”