Post Challenging Serum Cytokine Profile (Th1 & Th2) in the Vaccinated Mice (Balb/C) With a New Formulation of Leishmania major Antigen

Abstract

In this study, the LBT Group has statistical differences regarding IL-12 and IL-10 from the other Groups.

Measurement of Serum Levels of Interleukin 17 and 23 in the Immune System with Different Amounts of Spleens White Pulp Size after Inoculation of New Leishmania Vaccine in Susceptible Mice

“…In essence, induction or fortification of a satisfactory immune response against leishmania parasites requires a potent and harmless vaccine which could be used to immunized susceptible species. According to our present and previous experiments on Balb/C mice [20][21][22][23][24], the vaccine seem to be safe and effective; 100% viability was recorded in all groups vaccinated with different doses of antigen (100 µg/0.1 ml or 200 µg/0.1 ml) with BCG or alcoholic extract of T. polium and or both as adjuvant. The provisional vaccine induced satisfactory cytokine responses in such a way that are not only safe to the vaccinated animals (Balb/C mice), could protect vaccinated subjects against challenging with live leishmania parasite as shown previously [30,31].…”

“…BCG adjuvant for each injection dose is included 2 × 10⁵ CFU/0.1 ml. To prepare Teucrium adjuvant Palm, 400 mg of Teucrium polium alcoholic extract in 1 ml distilled water without endotoxin deionized, 205 mg/0.1 ml was used for each of the doses of the leishmania antigens given above, and two injection doses containing 100 μg/0.1 ml antigens or 200 μg/0.1 ml containing adjuvants [20][21][22].…”

“…For detailed procedures please refer to Latifynia and collaborations [20][21][22] [23,24,25]. In brief, Balb/C and traditional white laboratory mice (n=40) were obtained at three months old from the Pasture institute.…”

Pre-challenge Evaluation of Immune Response in Serum Cytokines (Th1 and Th2) and T-cell Markers (CD4, CD8, CD3, and CD25) Following Administration of New Formulated Leishmania Vaccine in Balb/c Mice

“…For details of the procedure, please refer to the previous studies by Latifynia et al [21][22][23][24][25][26]. In time, the harvested parasites were diluted to a concentration of 5.92 × 10 10 parasites per milliliter.…”

“…Also, in other studies, our new formulated provisional vaccine was fortified by using BCG and the alcoholic extract of the Teucrium polium plant as an adjuvant in Balb/c mice, and after that the vaccinated subjects were challenged with live promastigote that were harvested from the culture medium. In this experiment, Types 1 and 2 cytokines, spleen changes, and rate of survival were evaluated [24][25][26]. The findings indicated the safety, efficacy, and productivity of the new leishmania vaccine.…”