Detection and identification of bacterial soft rot of potato Pectobacterium carotovorum subsp. carotovorum using specific PCR primers in Jordan

Abu-Obeid, Ibtihal; Khlaif, Hamed; Salem, Nidà

doi:10.5897/ajar2017.12620

Cited by 5 publications

(12 citation statements)

References 17 publications

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“…On the other hand P. c. subsp. carotovorum is spread on warmer regions and attacks many other types of vegetables in addition to potato [16,2].…”

Section: Causal Agentsmentioning

confidence: 99%

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Soft rot disease in Jordan: A Review

2019

Adv. Environ. Biol

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Bacterial soft rot is one of the most destructive diseases of vegetables that occur in Jordan and in many regions of the world. It infects vegetables in the field, during transit and in storage causing great losses depending on the crop and prevailing of favorable conditions of high humidity coupled with high temperature. Generally, various bacterial species are known to be agents causing soft rot, in Jordan it was mainly caused by the pectolytic Pectobacterium carotovorum subsp. carotovorum in addition to Pseudomonas fluorescens, and different Enterobacteriaceae genera such as Enterobacter spp., Serratia spp., and Klebsiella spp., the disease was recorded in all vegetable growing regions and during different growing seasons, with a wide host range infecting many vegetable species belonging to all families causing decay of fleshy tissues. On the other hand, Pectobacterium carotovorum subsp. atroseptecum was found to have a restricted host range of potato and was reported in cool temperate regions or early in the season where the cool temperature is prevailing in potato growing regions. The detection and identification was done by different traditional methods of biochemical and physiological tests in addition to molecular techniques such as amplification of different regions by using different sets of primers. The bacterium enters the host tissues through natural opening, injures; caused by biotic and abiotic causal agents such as insects or by wrong practices during harvesting causing decay of tissues due to pectolic enzymes secreted by the bacterium, the tissues become soft and slimy where this is the same appearance on most of hosts. Control of the disease includes more effective and sustainable bacterial disease control strategies by applying different measures that reduce wounds and store vegetables in clean stores with low humidity and cold temperature and other physical and chemical methods which is discussed in more details. This review illustrates and discusses the disease status in Jordan through presenting an overview of all the results of studies done on this disease over many years.

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“…On the other hand P. c. subsp. carotovorum is spread on warmer regions and attacks many other types of vegetables in addition to potato [16,2].…”

Section: Causal Agentsmentioning

confidence: 99%

“…While Erwinia carotovora subsp. atroseptica the causal agent of black leg disease was found to be prevalent in cool regions and is restricted to potato, causing blackleg disease in winter in the Jordan Valley region and early spring in the uplands [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Soft rot disease in Jordan: A Review

2019

Adv. Environ. Biol

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“…The bits of surface sterilized tissues were transferred onto the surface of loop and the resulted suspension was streaked on the surface of dried media plates. The media used for isolation were selective media according to the suspected pathogen as for example; D1 media was used in the case of crown gall (Fakhouri and Khlaif, 1996;Al-Karablieh and Khlaif, 2002;Al-karabliah et al, 2006), BCBRVB in the case of fluorescent Pseudomonas (Abu-El Samen and Khlaif, 1999;Hijazin and Khlaif, 2005), Logan media in the case of Pectobacterium (Masa'adeh and Abu-Obeid et al, 2017), TZC in the case of Ralstonia solanacearum, NA and SX Agar in the case of the Xanthomonas campestris pv. campestris (Mahiar and , on the other hand, Nutrient Agar and KB media were used as common media for other pathogens.…”

Section: Isolation and Identification Of The Pathogenic Bacteriamentioning

confidence: 99%

“…Plant bacterial diseases spread in tropical and subtropical regions, and host range of bacterial diseases varies according to the disease, however some of them are with a wide host rang such as soft rot Pectobacterium carotovorum subsp. carotovorum (Omar andAbu-Obeid et al, 2017), and crown gall; Agrobacterium tumefaciens (Al-Karablieh and Khlaif, 2002;Al-Karablieh et al, 2006). On the other hand, some bacterial diseases had a narrow and restricted host ranges as in the case of black leg of potato P. carotovorum subsp.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of plant bacterial diseases in Jordan

Khlaif¹,

Abu-Obeid²,

Werikat³

2018

Afr. J. Agric. Res.

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Different studies were done in order to investigate the occurrence of bacterial diseases on different crops grown in Jordan during different growing seasons. Surveys were made and different bacterial diseases have been recorded based on symptoms and pathogenic nature. Morphological characters, biochemical tests and PCR detections were employed in order to detect and identify the causal agents of different inspected plant bacterial diseases. In addition, the distribution of the identified bacterial diseases, throughout the country was recorded. The results of our study revealed the occurrence of different bacterial diseases attacking different crops; grown in many growing regions throughout the country. Some of them were found to have a wide host range such as crown gall and soft rot, while others had a restricted host range as in the case of bacterial speck of tomato which was found to be restricted to tomato and black leg of potato. As a result of this study, the following diseases; angular leaf spot of cucumber, tomato speck, common blight, crown gall, soft rot, black rot, black leg and bacterial cankers resulted in high economic losses in yield. The spread of these diseases in the different areas in Jordan with different environmental conditions may result in the development of new races of the causal agents without developing typical symptoms making their diagnoses under field conditions difficult. Whereas the bacterial diseases needs deep and ideal studies in order to diagnose diseases, the diagnoses of these diseases act as the base for researchers to challenge and withdraw researches into the improvement of novel, more effective and sustainable bacterial disease control strategies.

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