Detection and identification of salmonellas from poultry-related samples by PCR

Oliveira, Sı́lvia Dias de; Santos, Luciana Ruschel dos; Schuch, Dulce Maria Tocchetto; Silva, A.B; Salle, Carlos Tadeu Pippi; Canal, Cláudio Wageck

doi:10.1016/s0378-1135(02)00028-7

Cited by 171 publications

(121 citation statements)

References 20 publications

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“…All 102 S. Enteritidis isolates contained the invasion gene invA, other studies having reported similar results (4,8), which was expected since the invA is an invasion gene conserved among Salmonella serotypes.…”

Section: Resultssupporting

confidence: 64%

“…DNA was extracted as previously described (4). PCR was performed with three sets of primer pairs: 139-141, specific for the invasion gene invA (4); PG 48-PG49, specific for the spvR gene (7); and VIR113-VIR561 specific for the spvC gene (5) The amplification of the invA gene fragment was carried out as previously described (4), the amplification conditions for the spvC gene fragment being similar except that the annealing temperature was 58°C.…”

Section: Pcrmentioning

confidence: 99%

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Detection of virulence genes in Salmonella Enteritidis isolated from different sources

Oliveira¹,

Rodenbusch²,

Michael³

et al. 2003

Braz. J. Microbiol.

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The presence of three virulence genes, invA, spvR, and spvC, was determined in Salmonella Enteritidis isolated from poultry, pigs, humans and food. All isolates were positive for the invA gene, with 91.2% being positive for spvR and 90.2% for spvC. There was no significant difference in the prevalence of the virulence genes between isolates from different sources. The results indicate that there is a putative high virulence potential for the S. Enteritidis isolates characterized.

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Section: Resultssupporting

confidence: 64%

Section: Pcrmentioning

confidence: 99%

Detection of virulence genes in Salmonella Enteritidis isolated from different sources

Oliveira¹,

Rodenbusch²,

Michael³

et al. 2003

Braz. J. Microbiol.

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show abstract

“…Additionally, a 488bp fragment of the sefA gene, specific for strains of S. enterica subsp. enterica serovar Enteritidis 17,18 , was also amplified. Finally, the same samples were used to amplify a 620bp fragment of the fliC (flageline) gene, with sequences specific to S. enterica subsp.…”

Section: Molecular Characterizationmentioning

confidence: 99%

“…Finally, the same samples were used to amplify a 620bp fragment of the fliC (flageline) gene, with sequences specific to S. enterica subsp. enterica serovar Typhimurium strains 17 . For PCR amplification, a final volume of 25µL containing 1.5mM MgCl 2 , 100µM deoxyribonucleoside triphosphate, 0.2µM of each oligonucleotide, and 1U of Taq ADN polymerase was used.…”

Section: Molecular Characterizationmentioning

confidence: 99%

Molecular characterization of Salmonella strains in individuals with acute diarrhea syndrome in the State of Sucre, Venezuela

Rodulfo

Donato

Luiggi

et al. 2012

Rev. Soc. Bras. Med. Trop.

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INTRODUCTION:In Venezuela, acute diarrheic syndrome (ADS) is a primary cause of morbi-mortality, often involving the Salmonella genus. Salmonella infections are associated with acute gastroenteritis, one of the most common alimentary intoxications, and caused by the consumption of contaminated water and food, especially meat. METHODS: Conventional and molecular methods were used to detect Salmonella strains from 330 fecal samples from individuals of different ages and both sexes with ADS. Polymerase chain reaction (PCR) was used for the molecular characterization of Salmonella, using invA, sefA, and fliC genes for the identification of this genus and the serotypes Enteritidis and Typhimurium, respectively. RESULTS: The highest frequency of individuals with ADS was found in children 0-2 years old (39.4%), and the overall frequency of positive coprocultures was 76.9%. A total of 14 (4.2%) strains were biochemically and immunologically identified as Salmonella enterica subsp. enterica, of which 7 were classified as belonging to the Enteritidis serotype, 4 to the Typhimurium serotype, and 3 to other serotypes. The S. enterica strains were distributed more frequently in the age groups 3-4 and 9-10 years old. CONCLUSIONS: The molecular characterization method used proved to be highly specific for the typing of S. enterica strains using DNA extracted from both the isolated colonies and selective enrichment broths directly inoculated with fecal samples, thus representing a complementary tool for the detection and identification of ADS-causing bacteria.

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“…Asian, American, European, and African health agencies have notified similar increases in such illnesses related to the consumption of eggs and poultry [3][4][5][6][7][8] .…”

Section: Introductionmentioning

confidence: 99%

Comparison of multiplex polymerase chain reaction and immunoassay to detect Salmonella spp., S. Typhimurium, and S. Enteritidis in Thai chicken meat

Rodpai¹,

Moongkarndi²,

Tungrugsasut³

et al. 2013

ScienceAsia

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Detection and identification of salmonellas from poultry-related samples by PCR

Cited by 171 publications

References 20 publications

Detection of virulence genes in Salmonella Enteritidis isolated from different sources

Detection of virulence genes in Salmonella Enteritidis isolated from different sources

Molecular characterization of Salmonella strains in individuals with acute diarrhea syndrome in the State of Sucre, Venezuela

Comparison of multiplex polymerase chain reaction and immunoassay to detect Salmonella spp., S. Typhimurium, and S. Enteritidis in Thai chicken meat

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