Detection and Localization of Rice stripe virus Gene Products In Vivo

Liang, Delin; Qu, Zhicai; Ma, Xiangqiang; Hull, Roger

doi:10.1007/s11262-005-1798-6

Cited by 39 publications

(41 citation statements)

References 34 publications

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“…The virus moves through the midgut, salivary gland, and ovary and is associated with amorphous or filamentous inclusions in the cytoplasm of midgut epithelial cells, salivary glands, and fat bodies (18,19). Using 454-FLX high-throughput pyrosequencing, Zhang et al (20) found that SBPH carries genes that play a role in regulating the innate immune systems, similar to those found in other insects, which may be involved in defense against viral infection.…”

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confidence: 88%

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Rice Stripe Tenuivirus Nonstructural Protein 3 Hijacks the 26S Proteasome of the Small Brown Planthopper via Direct Interaction with Regulatory Particle Non-ATPase Subunit 3

et al. 2015

J Virol

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The ubiquitin/26S proteasome system plays a vital role in regulating host defenses against pathogens. Previous studies have highlighted different roles for the ubiquitin/26S proteasome in defense during virus infection in both mammals and plants, but their role in the vectors that transmit those viruses is still unclear. In this study, we determined that the 26S proteasome is present in the small brown planthopper (SBPH) (Laodelphgax striatellus) and has components similar to those in plants and mammals. There was an increase in the accumulation of Rice stripe virus (RSV) in the transmitting vector SBPH after disrupting the 26S proteasome, indicating that the SBPH 26S proteasome plays a role in defense against RSV infection by regulating RSV accumulation. Yeast two-hybrid analysis determined that a subunit of the 26S proteasome, named RPN3, could interact with RSV NS3. Transient overexpression of RPN3 had no effect on the RNA silencing suppressor activity of RSV NS3. However, NS3 could inhibit the ability of SBPH rpn3 to complement an rpn3 mutation in yeast. Our findings also indicate that the direct interaction between RPN3 and NS3 was responsible for inhibiting the complementation ability of RPN3. In vivo, we found an accumulation of ubiquitinated protein in SBPH tissues where the RSV titer was high, and silencing of rpn3 resulted in malfunction of the SBPH proteasome-mediated proteolysis. Consequently, viruliferous SBPH in which RPN3 was repressed transmitted the virus more effectively as a result of higher accumulation of RSV. Our results suggest that the RSV NS3 protein is able to hijack the 26S proteasome in SBPH via a direct interaction with the RPN3 subunit to attenuate the host defense response. IMPORTANCEWe show, for the first time, that the 26S proteasome components are present in the small brown planthopper and play a role in defense against its vectored plant virus (RSV). In turn, RSV encodes a protein that subverts the SBPH 26S proteasome via direct interaction with the 26S proteasome subunit RPN3. Our results imply that the molecular arms race observed in plant hosts can be extended to the insect vector that transmits those viruses. Rice stripe virus (RSV), one of the most destructive pathogens of rice production, has been responsible for numerous epidemics since it was first described in Japan in 1897 (1-3). RSV is the type member of the Tenuivirus genus, and the viral genome consists of four single-stranded RNA segments which range in size from approximately 8.9 to 2.1 kb (4, 5). RNA 1 is negative sense and encodes a putative RNA-dependent RNA polymerase. RNAs 2, 3, and 4 are ambisense, and each contains two open reading frames (ORFs), with one on the viral RNA strand (vRNA) and the second on the viral cRNA strand (vcRNA) (6). RSV vRNA 2 encodes a membrane-associated protein that reportedly is an RNA silencing suppressor and interacts with suppressor of gene silencing 3 (SGS3) (7). vcRNA 2 encodes a glycoprotein (NSvc2) which when expressed in insect cells is displayed on the membrane su...

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confidence: 88%

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confidence: 99%

Rice Stripe Tenuivirus Nonstructural Protein 3 Hijacks the 26S Proteasome of the Small Brown Planthopper via Direct Interaction with Regulatory Particle Non-ATPase Subunit 3

et al. 2015

J Virol

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“…The cloned NS3 gene was composed of 636 nucleotides and 211 deduced amino acids. The nucleotide sequence generally shared a high degree of identity, ranging from 97 to 99%, with other NS3 genes already isolated from Korea (Lian et al, 2011), Japan and China (Jonson et al, 2009;Liang et al, 2005). The most similar nucleotide sequence already registered with GenBank, which differs in only two nucleotides, was Accession Y11095 from an isolate from Japan (Fig.…”

Section: Resultsmentioning

confidence: 73%

“…The open reading frame (ORF) consists of 636 nucleotides and 211 amino acids. The NS3 genes compared with YG-JN1 were selected from GenBank (NCBI) only with a high degree of similarity such as Y11095 (Liang et al, 2005), FJ602686 (Jonson et al, 2009), FN650803 (unpublished) and EF198724 (Xiong et al, 2009). Sanisbury and Lomonossoff, 2008).…”

Section: Resultsmentioning

confidence: 99%

Effect of Rice stripe virus NS3 on Transient Gene Expression and Transgene Co-Silencing

Sohn¹,

Huh²,

Kim³

et al. 2011

The Plant Pathology Journal

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Nonstructural protein 3 (NS3) encoded by RNA3 of Rice stripe virus (RSV), known to be a suppressor of gene silencing, was cloned and sequenced. The cloned NS3 gene is composed of 636 nucleotides encoding 211 deduced amino acids, and showed a high degree of similarity with the equivalent genes isolated from Korea, Japan and China. The NS3 gene promoted the enhancement of transient gene expression and suppressed transgene co-silencing. In the transient GFP expression via agroinfiltration, GFP expression was dramatically enhanced in terms of both protein yield and expression period in the presence of NS3. The highest accumulation of GFP protein reached to 6.8% of total soluble proteins, which corresponded to a two-fold increase compared to that obtained in the absence of NS3. In addition, NS3 significantly suppressed the initiation of GFP co-silencing induced by the additive GFP infiltration in GFP-transgenic Nicotiana benthamiana. The NS3 gene was also found to be a stronger suppressor than Cucumber mosaic virus 2b. These observations are believed to be derived from the strong suppressive effect of NS3 on gene silencing, and indicate that NS3 could be used as an effective enhancer for the rapid production of foreign proteins in plants.

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“…In a previous study, the timing of SP expression was earlier than CP (Wu et al, 2006). Furthermore, the expression of SP was limited to young leaf tissues early in the infection cycle (Liang et al, 2005). Thus, these results indicate that SP, a disease-specific protein which accumulates in infected plants, may be easily suppressed by antisense mediated RNA silencing.…”

Section: Rt-pcr Analysis Of Invading Viral Gene Expressionmentioning

confidence: 74%

Suppression of NS3 and MP Is Important for the Stable Inheritance of RNAi-Mediated Rice Stripe Virus (RSV) Resistance Obtained by Targeting the Fully Complementary RSV-CP Gene

Park

Choi

Kwak

et al. 2012

Molecules and Cells

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Rice stripe virus (RSV) is a viral disease that seriously impacts rice production in East Asia, most notably in Korea, China, and Japan. Highly RSV-resistant transgenic japonica rice plants were generated using a dsRNAi construct designed to silence the entire sequence region of the RSV-CP gene. Transgenic rice plants were inoculated with a population of viruliferous insects, small brown planthoppers (SBPH), and their resistance was evaluated using ELISA and an infection rate assay. A correlation between the expression of the RSV-CP homologous small RNAs and the RSV resistance of the transgenic rice lines was discovered. These plants were also analyzed by comparing the expression pattern of invading viral genes, small RNA production and the stable transmission of the RSV resistance trait to the T3 generation. Furthermore, the agronomic trait was stably transmitted to the T4 generation of transgenic plants.

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Detection and Localization of Rice stripe virus Gene Products In Vivo

Cited by 39 publications

References 34 publications

Rice Stripe Tenuivirus Nonstructural Protein 3 Hijacks the 26S Proteasome of the Small Brown Planthopper via Direct Interaction with Regulatory Particle Non-ATPase Subunit 3

Rice Stripe Tenuivirus Nonstructural Protein 3 Hijacks the 26S Proteasome of the Small Brown Planthopper via Direct Interaction with Regulatory Particle Non-ATPase Subunit 3

Effect of Rice stripe virus NS3 on Transient Gene Expression and Transgene Co-Silencing

Suppression of NS3 and MP Is Important for the Stable Inheritance of RNAi-Mediated Rice Stripe Virus (RSV) Resistance Obtained by Targeting the Fully Complementary RSV-CP Gene

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