Suppression of NS3 and MP Is Important for the Stable Inheritance of RNAi-Mediated Rice Stripe Virus (RSV) Resistance Obtained by Targeting the Fully Complementary RSV-CP Gene

Park, Hyang-Mi; Choi, Mansoo; Kwak, Do‐Yeon; Lee, Bong-Choon; Lee, Jonghee; Kim, Myeong-Ki; Kim, Yeon-Gyu; Shin, Dong-Bum; Park, Soon-Ki; Kim, Yul-Ho

doi:10.1007/s10059-012-2185-5

Cited by 15 publications

(5 citation statements)

References 46 publications

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“…In transgenic tobacco expressing hairpin RNA derived from TMV movement protein (MP) or CMV replication protein, two T4 transgenic lines with single copy were completely resistant to the corresponding virus ( Hu et al, 2011 ). In rice, strong resistance is induced in transgenic rice plants expressing hairpin RNA of the viral genes, such as RDV viroplasm matrix protein Pns12 ( Shimizu et al, 2009 ), RSV CP ( Park et al, 2012 ), Rice gall dwarf virus Pns9 ( Shimizu et al, 2012 ), Rice grassy stunt virus CP or MP ( Shimizu et al, 2013 ). In transgenic maize, expressing the hairpin structure transcribed from the CP gene of Sugarcane mosaic virus (SCMV) led to inhibition of SCMV infection, although to varying degrees ( Gan et al, 2014 ).…”

Section: The Application Of Virus-derived Small Rnasmentioning

confidence: 99%

Biogenesis, Function, and Applications of Virus-Derived Small RNAs in Plants

Zhang

et al. 2015

Front. Microbiol.

117

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RNA silencing, an evolutionarily conserved and sequence-specific gene-inactivation system, has a pivotal role in antiviral defense in most eukaryotic organisms. In plants, a class of exogenous small RNAs (sRNAs) originating from the infecting virus called virus-derived small interfering RNAs (vsiRNAs) are predominantly responsible for RNA silencing-mediated antiviral immunity. Nowadays, the process of vsiRNA formation and the role of vsiRNAs in plant viral defense have been revealed through deep sequencing of sRNAs and diverse genetic analysis. The biogenesis of vsiRNAs is analogous to that of endogenous sRNAs, which require diverse essential components including dicer-like (DCL), argonaute (AGO), and RNA-dependent RNA polymerase (RDR) proteins. vsiRNAs trigger antiviral defense through post-transcriptional gene silencing (PTGS) or transcriptional gene silencing (TGS) of viral RNA, and they hijack the host RNA silencing system to target complementary host transcripts. Additionally, several applications that take advantage of the current knowledge of vsiRNAs research are being used, such as breeding antiviral plants through genetic engineering technology, reconstructing of viral genomes, and surveying viral ecology and populations. Here, we will provide an overview of vsiRNA pathways, with a primary focus on the advances in vsiRNA biogenesis and function, and discuss their potential applications as well as the future challenges in vsiRNAs research.

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Section: The Application Of Virus-derived Small Rnasmentioning

confidence: 99%

Biogenesis, Function, and Applications of Virus-Derived Small RNAs in Plants

Zhang

et al. 2015

Front. Microbiol.

117

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“…ice stripe virus (RSV), one of the most destructive pathogens of rice production, has been responsible for numerous epidemics since it was first described in Japan in 1897 (1)(2)(3). RSV is the type member of the Tenuivirus genus, and the viral genome consists of four single-stranded RNA segments which range in size from approximately 8.9 to 2.1 kb (4,5).…”

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confidence: 99%

Rice Stripe Tenuivirus Nonstructural Protein 3 Hijacks the 26S Proteasome of the Small Brown Planthopper via Direct Interaction with Regulatory Particle Non-ATPase Subunit 3

et al. 2015

J Virol

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The ubiquitin/26S proteasome system plays a vital role in regulating host defenses against pathogens. Previous studies have highlighted different roles for the ubiquitin/26S proteasome in defense during virus infection in both mammals and plants, but their role in the vectors that transmit those viruses is still unclear. In this study, we determined that the 26S proteasome is present in the small brown planthopper (SBPH) (Laodelphgax striatellus) and has components similar to those in plants and mammals. There was an increase in the accumulation of Rice stripe virus (RSV) in the transmitting vector SBPH after disrupting the 26S proteasome, indicating that the SBPH 26S proteasome plays a role in defense against RSV infection by regulating RSV accumulation. Yeast two-hybrid analysis determined that a subunit of the 26S proteasome, named RPN3, could interact with RSV NS3. Transient overexpression of RPN3 had no effect on the RNA silencing suppressor activity of RSV NS3. However, NS3 could inhibit the ability of SBPH rpn3 to complement an rpn3 mutation in yeast. Our findings also indicate that the direct interaction between RPN3 and NS3 was responsible for inhibiting the complementation ability of RPN3. In vivo, we found an accumulation of ubiquitinated protein in SBPH tissues where the RSV titer was high, and silencing of rpn3 resulted in malfunction of the SBPH proteasome-mediated proteolysis. Consequently, viruliferous SBPH in which RPN3 was repressed transmitted the virus more effectively as a result of higher accumulation of RSV. Our results suggest that the RSV NS3 protein is able to hijack the 26S proteasome in SBPH via a direct interaction with the RPN3 subunit to attenuate the host defense response. IMPORTANCEWe show, for the first time, that the 26S proteasome components are present in the small brown planthopper and play a role in defense against its vectored plant virus (RSV). In turn, RSV encodes a protein that subverts the SBPH 26S proteasome via direct interaction with the 26S proteasome subunit RPN3. Our results imply that the molecular arms race observed in plant hosts can be extended to the insect vector that transmits those viruses. Rice stripe virus (RSV), one of the most destructive pathogens of rice production, has been responsible for numerous epidemics since it was first described in Japan in 1897 (1-3). RSV is the type member of the Tenuivirus genus, and the viral genome consists of four single-stranded RNA segments which range in size from approximately 8.9 to 2.1 kb (4, 5). RNA 1 is negative sense and encodes a putative RNA-dependent RNA polymerase. RNAs 2, 3, and 4 are ambisense, and each contains two open reading frames (ORFs), with one on the viral RNA strand (vRNA) and the second on the viral cRNA strand (vcRNA) (6). RSV vRNA 2 encodes a membrane-associated protein that reportedly is an RNA silencing suppressor and interacts with suppressor of gene silencing 3 (SGS3) (7). vcRNA 2 encodes a glycoprotein (NSvc2) which when expressed in insect cells is displayed on the membrane su...

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“…Rice stripe disease is one of most devastating viral diseases of rice in East Asia 17 18 . Infected plants often have chlorotic stripes or mottling and necrotic streaks in the newly expanded leaves and growth is stunted 17 19 20 .…”

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confidence: 99%

Interaction of HSP20 with a viral RdRp changes its sub-cellular localization and distribution pattern in plants

Xiang

Yang

et al. 2015

Sci Rep

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Small heat shock proteins (sHSPs) perform a fundamental role in protecting cells against a wide array of stresses but their biological function during viral infection remains unknown. Rice stripe virus (RSV) causes a severe disease of rice in Eastern Asia. OsHSP20 and its homologue (NbHSP20) were used as baits in yeast two-hybrid (YTH) assays to screen an RSV cDNA library and were found to interact with the viral RNA-dependent RNA polymerase (RdRp) of RSV. Interactions were confirmed by pull-down and BiFC assays. Further analysis showed that the N-terminus (residues 1–296) of the RdRp was crucial for the interaction between the HSP20s and viral RdRp and responsible for the alteration of the sub-cellular localization and distribution pattern of HSP20s in protoplasts of rice and epidermal cells of Nicotiana benthamiana. This is the first report that a plant virus or a viral protein alters the expression pattern or sub-cellular distribution of sHSPs.

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Suppression of NS3 and MP Is Important for the Stable Inheritance of RNAi-Mediated Rice Stripe Virus (RSV) Resistance Obtained by Targeting the Fully Complementary RSV-CP Gene

Cited by 15 publications

References 46 publications

Biogenesis, Function, and Applications of Virus-Derived Small RNAs in Plants

Biogenesis, Function, and Applications of Virus-Derived Small RNAs in Plants

Rice Stripe Tenuivirus Nonstructural Protein 3 Hijacks the 26S Proteasome of the Small Brown Planthopper via Direct Interaction with Regulatory Particle Non-ATPase Subunit 3

Interaction of HSP20 with a viral RdRp changes its sub-cellular localization and distribution pattern in plants

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