Detection and molecular characteristics of bovine rotavirus A in dairy calves in China

Li, Xiaoying; Yan, Nan; Yue, Hua; Wang, Yuanwei; Zhang, Bin; Tang, Cheng

doi:10.4142/jvs.2021.22.e69

Cited by 13 publications

(9 citation statements)

References 34 publications

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“…However, the P[7] genotype first appearing in China was detected in Korea in 2004–2005 but has not been detected since 2014. A recent study suggests that BoRVA circulates widely among dairy calves in China, and the dominant genotype is G6P[1] [ 26 ]; however, the most prevalent genotype in Korea is G6P[5], and the P[1] genotype has not yet emerged.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Bovine Group A Rotavirus Strains Circulating in Korean Calves during 2014 and 2018

Park

Choe

et al. 2022

Animals

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The purpose of this study was to investigate annual changes in BoRVA strains by examining the VP4 and VP7 genes of rotaviruses in Korean calves. Between 2014 and 2018, 35 out of 138 samples of calf diarrhea feces collected nationwide were positive for BoRVA. Further genetic characterization of the VP7 and VP4 genes of 35 BoRVA isolates identified three different G-genotypes (G6, G8, and G10) and two different P genotypes (P[5] and P[11]). The G6 genotype was most common (94.3%) in BoRVA-positive calves, followed by the P[5] genotype (82.9%). Four genotypes comprised combinations of VP4 and VP7: 80% were G6P[5], 14.2% were G6P[11], 2.9% were G8P[5], and 2.9% were G10P[11]. Susceptibility to infection was highest in calves aged < 10 days (35%) and lowest in calves aged 30–50 days (15.4%). The data presented herein suggest that the G6P[5] genotype is the main causative agent of diarrhea in Korean calves. In addition, it is predicted that G6P[5] will continue to act as a major cause of diarrhea in Korean calves.

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Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Bovine Group A Rotavirus Strains Circulating in Korean Calves during 2014 and 2018

Park

Choe

et al. 2022

Animals

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Section: Introductionmentioning

confidence: 99%

“…Neonatal calf diarrhea, which has the first place in calf deaths, causes significant economic losses in dairy farming around the world, due to treatment costs, labor, poor performance, growth retardation and deaths [ 1 ]. Despite scientific studies and chemotherapeutic advances on neonatal calf diarrhea, the disease still leads to high morbidity and mortality [ 1 , 2 ]. Diarrhea of especially with infectious origin (bacterial, viral and parasitic) occurs in the neonatal period, is commonly caused by E. coli , cryptosporidium, rotavirus and coronaviruses.…”

Section: Introductionmentioning

confidence: 99%

Characterization of bovine rotavirus isolates from diarrheic calves in Türkiye

Ates

Yeşilbağ

2023

Mol Biol Rep

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Background Neonatal calf diarrhea, which is the most common cause in calf deaths, leads to significant economic losses in dairy farming around the world. Diarrhea develops due to infectious and non-infectious reasons. Group A Rotaviruses (RVA) are the leading and predisposing factor for acute neonatal gastroenteritis. Methods and results In this study, 20 diarrheic fecal samples were collected from one farm in Balıkesir province of Turkey. During virus isolation, a total of 2 stool samples were detected to produce cytopathogenic effects in MA-104 cell line. The two samples (RV-36, RV-38) were tested positive with antigen ELISA kits detecting RVA antigens. In order to detect the presence of rotavirus viral nucleic acid in cell supernatants, VP6 gene region-specific RT-PCR test was performed and the samples RV-36 and RV-38 were positive for RVA viral nucleic acid. By RT-PCR using genotype specific primers, both the isolates RV-36 and RV-38 formed amplicons compatible with G10 and P[11] genotypes of RVA. RVA nucleic acids segments were also visualized by poliacrilamide gel electrophoresis (PAGE) method. The phylogenetic tree constructed according to the VP6 gene region showed that these isolates were in the Rotavirus A group and in the I2 cluster same as other bovine and some human RVA isolates. Conclusion Succesful isolation of RVA G10P[11] was echieved in the cattle farm. As rotaviruses play the most important role in the etiology of diarrhea in newborn calves respected genotype G10P[11] should be considered in selection of the vaccines applied to the dams. Those isolates can be further evaluated as vaccine candidate.

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“…Several G genotypes (G6, G8, and G10) and P genotypes (P [1], P [5], P [7], and P [11]) have been identi ed in the Chinese cattle population. Notably, the only study conducted previously revealed that G6 and P [5] are common G and P genotypes among dairy calves in certain regions of China, with G6P being the dominant genotype combination (Liu et al, 2021). G10 is only popular in some regions.…”

Section: Introductionmentioning

confidence: 99%

Molecular characteristics of VP4 and VP7 of a Rotavirus A in cattle in Yunnan Province, China

li,

Zhang,

Duan

et al. 2023

Preprint

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Rotavirus is classified within the family Reoviridae, subfamily Sedoreovirinae, and the genus Rotavirus. This genus encompasses eight groups, denoted as species A to H, with Group A holding particular significance due to its widespread prevalence among both human and animal populations. Among these, Bovine rotavirus (BoRV) stands out as the leading cause of substantial morbidity, mortality, and economic losses in neonatal calves.Rotavirus is classified within the family Reoviridae, subfamily Sedoreovirinae, and the genus Rotavirus. This genus encompasses eight groups, denoted as species A to H, with Group A holding particular significance due to its widespread prevalence among both human and animal populations. Among these, Bovine rotavirus (BoRV) stands out as the leading cause of substantial morbidity, mortality, and economic losses in neonatal calves. In order to identify the etiology of cattle with diarrheal diseases and further enrich the epidemiological data of bovine rotavirus (BRV) in China, RT-PCR was used to identify virus pathogens from 10 diarrheal cattle fecal samples. Positive samples were inoculated in MA-104 cells, generating stable cytopathologies from the sixth generation. Basic physicochemical properties, structural functions and functions of the VP4/VP7 proteins of the virus were predicted and analyzed by indirect immunofluorescence test (IFA). The results identified the isolated virus was BRV and it was named RVA/Cattle/CHN/YN1/2021/G8P. Following RT-PCR amplification, sequencing and splicing of 11 gene segments of this strain, homology and typing analyses were conducted. A genetic tree of isolated strains was constructed based on VP7 and VP4 sequences, and the genetic evolutionary relationship and the variation of amino acid sequences were analyzed. The results showed that among 11 fragments of the YN-1 genome, 5 segments showed high nucleotide identity with a deer strain, 2 segments with human, 1 with U.S. vaccine strain; 7 segments highly identified with a U.S. strain, among them 5 segments were highly identified with strain C/Cervidae/United States/14-02218-2/2014. Bioinformatics analysis results indicated that both VP4 and VP7 proteins are stable, hydrophilic, transmembrane and non-secretory proteins. The number of O-glycosylation, N-glycosylation, phosphorylation sites were 150 and 59, 3, 102 and 34 in VP4 and VP7, respectively. Subcellular localization is located in the plasma membrane, with α-Spiral and extension as the main structures, with irregular curls and β-The corner running through it. Therefore, the genome constellation of this strain was determined as G8-P[1]-I2-R2-C2-M2-A3-N2-T6-E2-H. The study results lay a foundational work for the development of vaccines, and detection kits, which also support the effective prevention and control of BRV infection in China in the near future.

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Detection and molecular characteristics of bovine rotavirus A in dairy calves in China

Cited by 13 publications

References 34 publications

Genetic Diversity of Bovine Group A Rotavirus Strains Circulating in Korean Calves during 2014 and 2018

Genetic Diversity of Bovine Group A Rotavirus Strains Circulating in Korean Calves during 2014 and 2018

Characterization of bovine rotavirus isolates from diarrheic calves in Türkiye

Molecular characteristics of VP4 and VP7 of a Rotavirus A in cattle in Yunnan Province, China

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