Detection and molecular identification of Leucocytozoon and Plasmodium species from village chickens in different areas of Myanmar

Win, Shwe Yee; Chel, Hla Myet; Hmoon, Myint Myint; Htun, Lat Lat; Bawm, Saw; Win, Mar Mar; Shiro, Motoo; Nonaka, Nariaki; Katakura, Ken

doi:10.1016/j.actatropica.2020.105719

Cited by 19 publications

(19 citation statements)

References 27 publications

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“…There have been studies of genetic diversity of Leucocytozoon sp. based on the mitochondrial gene sequences in several countries, and almost all of these studies focused on the cytb gene [ 8 , 18 , 29 , 44 ]. However, there has been no information available regarding the genetic diversity and phylogeny of L. sabrazesi mitochondrial genes in Thailand until now.…”

Section: Discussionmentioning

confidence: 99%

“…A phylogenetic analysis was carried out to display the relationship between individual and multi-locus genes of mitochondria determining the detection of L. sabrazesi . Moreover, the coxI gene has been employed to detect te infection of P. gallinaceum and P. juxtanucleare in chickens from Bongti and Tha Sao districts in Kanchanaburi province located near the Chacheongsao province of Thailand which are reported about P. gallinaceum [ 34 ] and near at the border of Myanmar which are reported regarding P. juxtanucleare in chickens [ 44 ]. Regarding three mitochondrial nucleotide sequences, our results indicated the highest sequence similarity to L. sabrazesi and some co-infected with P. gallinaceum and P. juxtanucleare.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, haplotype diversity indicated the close genetic relationship among L. sabrazesi. detected in Thailand, Malaysia and Myanmar [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

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Molecular genetic diversity and bioinformatic analysis of Leucocytozoon sabrazesi based on the mitochondrial genes cytb, coxI and coxIII and co-infection of Plasmodium spp.

et al. 2022

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Leucocytozoon sabrazesi is an intracellular haemoprotozoan parasite responsible for leucocytozoonosis, which is transmitted by insect vectors and affects chickens in tropical and subtropical areas in many countries. It causes huge economic losses due to decreased meat and egg production. In the present study, we used nested PCR to determine the genetic diversity of L. sabrazesi based on the cytb, coxI, coxIII and concatenated genes in chickens in Thailand. In addition, we found co-infections between L. sabrazesi and Plasmodium spp. (P. gallinaceum or P. juxtanucleare) in chickens that were not identified by microscopic examination of blood smears. The phylogenetic analysis indicated that L. sabrazesi cytb and coxIII genes were conserved with similarity ranging from 99.9 to 100% and 98 to 100%, respectively whereas the coxI gene was diverse, with similarities ranging from 97 to 100%. These findings ascertained the nucleotide analysis of the cytb, coxI, coxIII and concatenated sequences in which 4, 8, 10 and 9 haplotypes were found, respectively. In addition, it was found that the large number of synonymous substitutions and conservative amino acid replacements in these mitochondrial genes occurred by non-synonymous substitution. The evolutionary analysis of the Ka/Ks ratio supported purifying selection and the negative values of both Fu’s Fs and Tajima’s D indicate selective sweep especially for the coxI gene. The entropy and Simplot analysis showed that the genetic variation in populations of Plasmodium spp. was higher than in Leucocytozoon. Hence, the nucleotide sequences of three mitochondrial genes could reflect the evolutionary analysis and geographic distribution of this protozoan population that switches hosts during its life cycle.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Molecular genetic diversity and bioinformatic analysis of Leucocytozoon sabrazesi based on the mitochondrial genes cytb, coxI and coxIII and co-infection of Plasmodium spp.

et al. 2022

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“…Microscopic histopathological examination is used to provide definitive diagnoses of L. caulleryi infections in chickens [ 24 , 25 ]. Histopathological studies of L. caulleryi in this study revealed numerous megaloschizonts in organs such as the pancreas, intestine, lung, and pectoral muscle.…”

Section: Discussionmentioning

confidence: 99%

Pathology and molecular characterization of Leucocytozoon caulleryi from backyard chickens in Khon Kaen Province, Thailand

2021

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Aim: The aim of this study was to characterize Leucocytozoon caulleryi from backyard chickens in Khon Kaen Province, Thailand. Materials and Methods: Tissue samples were collected from backyard chickens suspected to have leucocytozoonosis and subjected to histopathology examination. The BLAST Tool at NCBI GenBank (Basic Local Alignment Research Tools) (http://www.ncbi.nlm.nih.gov/BLAST) was used to identify the nucleotide sequence of the partial cytochrome c oxidase subunit I (cox I) gene. A Phylogenetic tree for analysis of L. caulleryi was constructed by using MEGA 7.0 software (https:// www.megasoftware.net/). Results: The necropsy results revealed the subcutaneous hemorrhages of pectoral muscles, multifocal hemorrhages of the thymus and pectoral muscles, hemorrhage of the proventriculus and peritoneal cavity, and megaloschizonts of the pancreas and intestine, including subcapsular hemorrhages of the liver. Microscopic examination revealed numerous megaloschizonts of Leucocytozoon spp. in the pectoral muscles, intestine, pancreas, and thymus. Molecular analysis of the partial cox I gene showed that the causal agent was closely related to L. caulleryi reported in Japan. Conclusion: From these results, L. caulleryi was determined to be the causal agent of leucocytozoonosis and was closely associated with L. caulleryi reported in Japan.

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“…Mathis and Leger (1909) first detected L. caulleryi in the blood of domestic fowl in the Tonkin region of Vietnam. Since then, relevant evidence from Malaysia ( Kuppuswany, 1936 ), Japan ( Akiba et al, 1958 ), Myanmar ( Win et al, 2020 ), and Taiwan ( Lee et al, 1966 ) has been presented. Through further investigation into leucocytozoonosis, Akiba (1960) discovered that the vector for L. caulleryi is a biting midge of the genus Culicoides specifically, Culicoides arakawae .…”

Section: Introductionmentioning

confidence: 99%

Effects of Artemisia annua on experimentally induced leucocytozoonosis in chickens

Chiang

Lin²,

Wang³

et al. 2022

Poultry Science

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Detection and molecular identification of Leucocytozoon and Plasmodium species from village chickens in different areas of Myanmar

Cited by 19 publications

References 27 publications

Molecular genetic diversity and bioinformatic analysis of Leucocytozoon sabrazesi based on the mitochondrial genes cytb, coxI and coxIII and co-infection of Plasmodium spp.

Molecular genetic diversity and bioinformatic analysis of Leucocytozoon sabrazesi based on the mitochondrial genes cytb, coxI and coxIII and co-infection of Plasmodium spp.

Pathology and molecular characterization of Leucocytozoon caulleryi from backyard chickens in Khon Kaen Province, Thailand

Effects of Artemisia annua on experimentally induced leucocytozoonosis in chickens

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