Detection and partial molecular characterization of Plum pox virus on almond trees in Turkey

İlbağı, Havva; Çıtır, Ahmet

doi:10.1007/s12600-014-0385-6

Cited by 17 publications

(8 citation statements)

References 13 publications

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“…Map showing the distribution in Turkey of Plum pox virus strains Dideron (D), Marcus (M), Turkey (T) and Recombinant (Rec) according to previous studies (Ulubaş Serçe et al ., ; İlbağı & Ç?t?r, ; Gürcan & Ceylan, ) and the collection sites of the 56 PPV ‐T isolates analysed in the present report. Further information on these 56 isolates is provided in Table .…”

Section: Methodsmentioning

confidence: 83%

“…In Turkey, PPV was discovered for the first time in Edirne in 1968, the region bordering Bulgaria, and soon after in Ankara (central Anatolia) in 1973 (Kurçman, ). Subsequently, it was detected in Marmara, Mediterranean and Aegean regions (Elibüyük, ; Ulubaş Serçe et al ., ; Akbaş et al ., ; İlbağı & Çıtır, ; Gürcan & Ceylan, ). Recently, sequence analysis was performed for characterization and strain identification of Turkish isolates.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, sequence analysis was performed for characterization and strain identification of Turkish isolates. The PPV‐T strain was found to be common in residential gardens in Ankara, İzmir, Istanbul, Kayseri, Konya and the Balkan region (Edirne, Kırklareli, and Tekirdağ) (İlbağı & Çıtır, ; Gürcan & Ceylan, ). Similarly, isolates of PPV‐D were identified in residential gardens of Aksaray, Bursa, Eskişehir, Konya, Istanbul and the Balkan region (Gürcan & Ceylan, ).…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity and molecular epidemiology of the T strain of Plum pox virus

et al. 2019

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Very limited information is available on the origin, diversity and evolution of Plum pox virus (PPV) 'Turkey' (T) strain. Phylogenetic analyses based on partial sequences of 421 isolates and complete genome sequences of 57 isolates, representing the geographical distribution of PPV-T in Turkey, revealed the existence of several monophyletic and, in some cases, geographically limited groups within the PPV-T strain (Ankara-Konya1-Kayseri, Ankara-Balkan, Istanbul, Konya2 and Balkan). PPV-T diversity (0.018%) was found to be greater than that of PPV strains D and Rec but lower than that of the M strain when including the newly described and divergent M-Istanbul isolates, suggesting a long evolutionary history for PPV-T. The European part of Turkey in the Balkans, close to Bulgaria where PPV was identified for the first time, appears as a likely centre of origin for PPV-T isolates. The colonization of various parts of Turkey by diverse isolates from that region, followed by secondary local spread, is the most likely scenario for the diffusion of PPV-T in Turkey.

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Section: Methodsmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic diversity and molecular epidemiology of the T strain of Plum pox virus

et al. 2019

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“…Almond (Prunus dulcis), although previously considered only an experimental host (24, 80), has recently been described as a natural host for PPV (62). PPV has also been observed on wild or ornamental species such as blackthorn (Prunus spinosa) (28, 63).…”

Section: Sharka Identity Cardmentioning

confidence: 99%

Sharka Epidemiology and Worldwide Management Strategies: Learning Lessons to Optimize Disease Control in Perennial Plants

Rimbaud

Dallot

Gottwald

et al. 2015

Annu. Rev. Phytopathol.

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Many plant epidemics that cause major economic losses cannot be controlled with pesticides. Among them, sharka epidemics severely affect prunus trees worldwide. Its causal agent, Plum pox virus (PPV; genus Potyvirus), has been classified as a quarantine pathogen in numerous countries. As a result, various management strategies have been implemented in different regions of the world, depending on the epidemiological context and on the objective (i.e., eradication, suppression, containment, or resilience). These strategies have exploited virus-free planting material, varietal improvement, surveillance and removal of trees in orchards, and statistical models. Variations on these management options lead to contrasted outcomes, from successful eradication to widespread presence of PPV in orchards. Here, we present management strategies in the light of sharka epidemiology to gain insights from this worldwide experience. Although focused on sharka, this review highlights more general levers and promising approaches to optimize disease control in perennial plants.

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“…Türkiye dışında sadece Arnavutluk'ta rapor edilen (Palmisano ve ark. 2015 Çıtır, 2014;Deligöz ve ark. 2015, Gürcan ve ark 2013b, Gürcan and Ceylan 2016b …”

Section: Bulgular Ve Tartışmaunclassified

Bursa’da plum pox virus (Şarka)'ün yaygınlığının ve genetik çeşitliliğinin belirlenmesi

Gürcan¹

2017

Anadolu Journal of Agricultural Sciences

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ÖZETPlum pox virus (PPV) tarafından oluşturulan Şarka hastalığı, sert çekirdekli meyve türlerinin en önemli viral hastalığı olarak görülmektedir. Son yıllarda yapılan nükleotid dizileme çalışmaları ile PPV'nin Türkiye'de şehir merkezi ev bahçelerinde sanıldığından daha fazla yaygınlık ve genetik çeşitlilik gösterdiği belirlenmiştir. Ev bahçeleri Türkiye tipi PPV-D ve PPV-T ırkları ile bulaşık iken, kapama bahçeler Avrupa tipi PPV-M ırkı ile bulaşıktır. Bursa ili Türkiye'de önemli bir şeftali ve fidan üretim bölgesi olup, aynı zamanda Avrupa'dan gelen fidanların Türkiye içinde dağıtım merkezidir. Bu nedenle iyi izlenmesi gereken bir ildir. Bu çalışmada, Bursa ilinde surveyler yapılmış, tespit edilen PPV-pozitif örneklere ait izolatlar, Türkiye'nin diğer bölgelerinde ve Avrupa'da PPV izolatları ile karşılaştırılmıştır. Bursa ili ve civarında, 50 kapama bahçeden 118 örnek DASI-ELISA ve RT-PCR yöntemleri ile test edilmiş, 32 bahçeden alınan 64 örnek pozitif bulunmuştur. Bu örneklerin 664 nt uzunluğunda P3-6K1 bölgesi ve 969 nt uzunluğunda CT-3'UTR bölgesi dizilenmiş, GenBank'a kayıtlı 200 adet izolat ile birlikte analiz edilmiştir. Otuz şeftali bahçesinden alınan 56 izolatın, Türkiye kapama bahçelerinde tespit edilen Avrupa PPV-M izolatı olduğu belirlenmiştir. Bursa'da iki erik bahçesinden alınan 8 adet izolat ise PPV-D olarak tespit edilmiş olup, bu izolatlar Türkiye tipi PPV-D izolatları ile aynı grupta yer almıştır. Sonuç itibariyle, Bursa kapama şeftali bahçelerinin Avrupa tipi PPV-M ile yaygın olarak bulaşık olduğu ve bu izolatların PPV'nin yeni giriş yaptığı Aydın, Denizli, Çanakkale, Isparta ve Kayseri illeri kapama bahçe izolatları ile yakın genetik benzerlik gösterdiği belirlenmiştir. Bu sonuç, PPV-M ırkının Türkiye'de yayılmasında, Bursa-Türkiye üzerinden olan fidan trafiğinin rolüne işaret etmiştir. Determination of prevalence and genetic diversity of plum pox virus (sharka) in peach orchards in BursaAnahtar Sözcükler: Şarka Şeftali Sert Çekirdekli meyveler PPV-Marcus PPV-Dideron ABSTRACT Plum pox virus (PPV) is the most important viral disease of stone fruit species. Recent nucleotide sequencing studies have revealed that PPV is prevalent and exhibits high genetic diversity in residential gardens of several provinces in Turkey. Trees in residential gardens have been found to be infected by Turkish type PPV-D and PPV-T while trees in professional orchards in several cities have been found to be affected by the European strain PPV-M. The province Bursa is main peach producer region in Turkey but moreover Bursa hosts big nursery industry and is the distribution center of seedlings imported from European countries. In this research, surveys were made in the orchards of Bursa and surrounding regions, and comparisons were made in order to investigate genetic relationships among the isolates of Bursa and other PPV isolates determined in Turkey as well as European PPV isolates. A total of 118 samples from 50 orchards were examined by DASI-ELISA and RT-PCR methods. Of the all, 64 samples from 32 orchards were fou...

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Detection and partial molecular characterization of Plum pox virus on almond trees in Turkey

Cited by 17 publications

References 13 publications

Genetic diversity and molecular epidemiology of the T strain of Plum pox virus

Genetic diversity and molecular epidemiology of the T strain of Plum pox virus

Sharka Epidemiology and Worldwide Management Strategies: Learning Lessons to Optimize Disease Control in Perennial Plants

Bursa’da plum pox virus (Şarka)'ün yaygınlığının ve genetik çeşitliliğinin belirlenmesi

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