Detection and Quantification of Major Phytochemical Markers for Standardization of Talinum Portulacifolium, Gomphrena Serrata, Alternanthera Sessilis and Euphorbia Heterophylla by HPLC

Abstract

Back ground: High-performance Liquid Chromatography is one of the major analytical techniques used in the quality control of phytochemicals. Objective: This research article presents the development of HPLC method to detect and quantify the major marker components, kaempferol, and quercetin from four plant species. Materials and Methods: HPLC method was developed for the qualitative and quantitative analysis of plant extracts by using orthophosphoric acid and methanol (95:5) at 370 nm for kaempferol, methanol and orthophosphoric acid (60:40) at 262nm for quercetin. Results: Kaempferol was detected from the hydro alcoholic extracts of Talinum portulacifolium leaves (RT 13.720, concentration 1.08 mg/ml) and flowers of Gomphrena serrata (RT 13.758, concentration 2.13mg/ml). Kaempferol was reported for the first time from Gomphrena serrata. Quercetin was separated and identified from the hydro alcoholic extracts Alternanthera sessilis stems (RT 6.503, concentration 0.01mg/ml). The hydroalcoholic extract of Euphorbia heterophylla stems (RT 6.588, concentration 0.01mg/ml) was also evaluated for the presence of quercetin. Conclusion: The method developed is very useful tool for qualifying and quantifying the plant specimens as well as their extracts.

Alternanthera sessilis: A Review of Literature on the Phytoconstituents, Traditional Usage and Pharmacological Activities of Green and Red Cultivar

“…[31,32] Consequently, ponnankanni name is given to this plant in India due to the fact that Indians from the south part belief that it can give the body a "golden sheen". [33] In Indonesia, ASG is employed to treat disorders of intestines such as dysentery and stomach related disease. [34,35] ASG was also used to treat indigestion associated with a poor functioning liver, bile related issue, persisting liver congestion, inflamed kidney pelvis, inflamed bladder, strangury, gonorrhea and snakebites in Sri Lanka.…”

Rice-Based Alcoholic Fermented Beverages of North-East India: Insight into Ethnic Preparation, Microbial Intervention, Ethnobotany, and Health Benefits

“…Zanthoxylum oxyphyllum Edgew. : skin disease, rheumatism, ulcer, pain, fever, and hypotension[36,73,99,102,[105][106][107][108][109][110][111][112][113] Willd. : infammation-linked diseases, such as hepatitis, appendicitis, and urethritis.…”