Detection and quantification of prostaglandin E2 in saliva by liquid chromatography-tandem mass spectrometry using microextraction by packed sorbent

Oliveira, Graça; Dionísio, Thiago José; Weckwerth, Giovana Maria; Sandrin, Viviane Silva Siqueira; Polanco, Nelson Leonel Hierro; Faria, Flávio Augusto Cardoso de; Santos, Carlos; Calvo, Ana Flávia Bissoto

doi:10.1016/j.prostaglandins.2022.106672

Cited by 4 publications

(22 citation statements)

References 21 publications

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“…The MEPS methodology was selected for the extraction of analytes from oral fluid samples and calibration curves. MEPS consists of the miniaturization of conventional SPE with a conditioned needle incorporated directly into an injector syringe (Trajan Scientific Australia Pty Ltd) which is an alternative to other extraction methodologies in different matrixes [ 36 ], thus demonstrating its specificity and reduction in matrix effects [ 37 , 38 ], including in oral fluid samples [ 16 , 21 , 27 , 39 , 40 ].…”

Section: Experimental Designmentioning

confidence: 99%

“…In this sense, several studies have shown that PGE 2 can be the main target of PK/PD studies. Changes in PGE 2 concentrations, on the other hand, can be used to quantify the influence of COX-2 after the administration of the studied non-steroidal anti-inflammatory drugs (NSAIDs) [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, this step requires more time and, sometimes, more plastic materials. Such sample treatment in the pre-analytical phase aims to remove matrix components that may interfere with the final analysis and, thus, isolate the specific analyte, thus promoting a clean and specific sample [ 21 , 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

“…MEPS is a miniaturization of solid-phase extraction (SPE) with a packed bed (1–2 mg). Although its applicability in oral fluid is recent, it has shown to be promising with some drugs [ 21 , 27 , 37 , 38 ].…”

Section: Introductionmentioning

confidence: 99%

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Liquid Chromatography-Tandem Mass Spectrometry Method for Detection and Quantification of Meloxicam and 5′-Carboxymeloxicam in Oral Fluid Samples

et al. 2023

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A sensitive, selective and particularly fast method of liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated for the determination of meloxicam and its main metabolite, 5′-carboxymeloxicam, in oral fluid samples. Meloxicam and its major metabolite were separated using a Shim-Pack XR-ODS 75 L × 2.0 column and C18 pre-column at 40 °C using a mixture of methanol and 10 mM ammonium acetate (80:20, v/v) with an injection flow rate of 0.3 mL/min. The total time of the analytical run was 5 min. Sixteen volunteers had oral fluid samples collected sequentially before and after taking a meloxicam tablet (15 mg) for up to 96 h. With the concentrations obtained, the pharmacokinetic parameters were determined using the Phoenix WinNonlin software. The parameters evaluated for meloxicam and 5′-carboxymeloxicam in the oral fluid samples showed linearity, accuracy, precision, medium-quality control (MQC-78.12 ng/mL), high-quality control (HQC-156.25 ng/mL), lower limits of quantification (LLOQ-0.6103 ng/mL), low-quality control (LQC-2.44 ng/mL), stability and dilution. Prostaglandin E2 (PGE2) was also detected and quantified in the oral fluid samples, demonstrating the possibility of a pharmacokinetic/pharmacodynamic (PK/PD) study with this methodology. All the parameters evaluated in the validation of the methodology in the oral fluid samples proved to be stable and within the possible variations in each of the described parameters. Through the data presented, the possibility of a PK/PD study was demonstrated, detecting and quantifying meloxicam, its main metabolite and PGE2 in oral fluid samples using LC-MS/MS.

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Section: Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Liquid Chromatography-Tandem Mass Spectrometry Method for Detection and Quantification of Meloxicam and 5′-Carboxymeloxicam in Oral Fluid Samples

et al. 2023

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“…PGE2, a naturally occurring lipid biomolecule with a small molecular size, presents challenges in molecular-level detection. However, earlier literature has made significant strides in sensitive and selective detection methods such as conventional chromatography, mass spectrometry (MS), enzyme-linked immunosorbent assay (ELISA), enzyme immunoassay (EIA), advanced single-molecule arrays (Simoa), , and electrochemical sensors. , However, all primarily rely on the use of either biorecognition elements or a costly centralized technique; there is an emerging consensus on the potential benefits of developing alternative approaches for molecular-level detection of PGE2. The integration of nanostructures, such as quantum dots (QDs), presents promising avenues for molecular diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Hydroxypropyl β-Cyclodextrin-Functionalized MoO₃ Quantum Dots as a Multifunctional Probe for PGE2 Detection, Bioimaging, and Oxidative Stress Management in Inflammatory Diseases

Pandey,

Balasubramanian Chellammal,

Krishnaswamy

et al. 2024

ACS Appl. Nano Mater.

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Prostaglandin E2 (PGE2) is an important biomarker for cancers and chronic inflammation-associated diseases. While PGE2 plays a crucial role in the inflammatory response, chronically elevated PGE2 levels may contribute to disease onset and oxidative stress. Herein, hydroxypropyl β-cyclodextrin (HPbCD)-functionalized MoO 3 quantum dots (QDs) were synthesized with a hydrophilic exterior and hydrophobic core. The QDs were characterized for their physicochemical, bioimaging, and biocompatible nature. The QDs exhibited potential reactive oxygen species (ROS) scavenging and bioimaging in Caenorhabditis elegans. Further, the QD's high binding affinity-based nanomolar detection of PGE2 (2−40 nM) was performed at physiological pH. The detection mechanism was investigated using Stern−Volmer and Lineweaver−Burk equations, where K q (8.9 × 10 15 and 2.85 × 10 14 L/mol/s) and K b 1.1 × 10 7 M −1 with 1:1 binding interaction. The energetically favorable and spontaneous binding of PGE2 to MoCD QDs suggested strong host−guest inclusionbased detection of PGE2. The biosensor performance was tested with a potent interferent and validated in clinical serum samples. The developed biocompatible nanoquantum probe with triad applications, including oxidative stress management, bioimaging, and ultrasensitive detection of PGE2, is envisaged to be a valuable tool in molecular diagnosis in treatment of inflammation.

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CYP2C9 Polymorphism Influence in PK/PD Model of Naproxen and 6-O-Desmethylnaproxen in Oral Fluid

et al. 2022

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Polymorphisms in CYP2C9 can significantly interfere with the pharmacokinetic (PK) and pharmacodynamic (PD) parameters of nonsteroidal anti-inflammatory drugs (NSAIDs), including naproxen. The present research aimed to study the PK/PD parameters of naproxen and its metabolite, 6-O-desmethylnaproxen, associated with allelic variations of CYP2C9. In our study, a rapid, selective, and sensitive Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) method was developed and validated for the determination of naproxen and its main metabolite, 6-O-desmethylnaproxen, in oral fluid. Naproxen and its main metabolite were separated using a Shim-Pack XR-ODS 75L × 2.0 column and C18 pre-column at 40 °C using a mixture of methanol and 10 mM ammonium acetate (70:30, v/v), with an injection flow of 0.3 mL/min. The total analytical run time was 3 min. The volunteers, previously genotyped for CYP2C9 (16 ancestral—CYP2C9 *1 and 12 with the presence of polymorphism—CYP2C9 *2 or *3), had their oral fluids collected sequentially before and after taking a naproxen tablet (500 mg) at the following times: 0.25, 0.5, 0.75, 1, 1.5, 2, 3, 4, 5, 6 8, 11, 24, 48, 72 and 96 h. Significant differences in the PK parameters (* p < 0.05) of naproxen in the oral fluid were: Vd/F (L): 98.86 (55.58–322.07) and 380.22 (261.84–1097.99); Kel (1/h): 0.84 (0.69–1.34) and 1.86 (1.09–4.06), in ancestral and mutated CYP2C9 *2 and/or *3, respectively. For 6-O-desmethylnaproxen, no PK parameters were significantly different between groups. The analysis of prostaglandin E2 (PGE2) proved to be effective and sensitive for PD parameters analysis and showed higher levels in the mutated group (p < 0.05). Both naproxen and its main metabolite, 6-O-desmethylnaproxen, and PGE2 in oral fluid can be effectively quantified using LC-MS/MS after a 500 mg oral dose of naproxen. Our method proved to be effective and sensitive to determine the lower limit of quantification of naproxen and its metabolite, 6-O-desmethylnaproxen, in oral fluid (2.4 ng/mL). All validation data, such as accuracy, precision, and repeatability intra- and inter-assay, were less than 15%. Allelic variations of CYP2C9 may be considered relevant in the PK of naproxen and its main metabolite, 6-O-desmethylnaproxen.

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Detection and quantification of prostaglandin E2 in saliva by liquid chromatography-tandem mass spectrometry using microextraction by packed sorbent

Cited by 4 publications

References 21 publications

Liquid Chromatography-Tandem Mass Spectrometry Method for Detection and Quantification of Meloxicam and 5′-Carboxymeloxicam in Oral Fluid Samples

Liquid Chromatography-Tandem Mass Spectrometry Method for Detection and Quantification of Meloxicam and 5′-Carboxymeloxicam in Oral Fluid Samples

Hydroxypropyl β-Cyclodextrin-Functionalized MoO₃ Quantum Dots as a Multifunctional Probe for PGE2 Detection, Bioimaging, and Oxidative Stress Management in Inflammatory Diseases

CYP2C9 Polymorphism Influence in PK/PD Model of Naproxen and 6-O-Desmethylnaproxen in Oral Fluid

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Detection and quantification of prostaglandin E2 in saliva by liquid chromatography-tandem mass spectrometry using microextraction by packed sorbent

Cited by 4 publications

References 21 publications

Liquid Chromatography-Tandem Mass Spectrometry Method for Detection and Quantification of Meloxicam and 5′-Carboxymeloxicam in Oral Fluid Samples

Liquid Chromatography-Tandem Mass Spectrometry Method for Detection and Quantification of Meloxicam and 5′-Carboxymeloxicam in Oral Fluid Samples

Hydroxypropyl β-Cyclodextrin-Functionalized MoO3 Quantum Dots as a Multifunctional Probe for PGE2 Detection, Bioimaging, and Oxidative Stress Management in Inflammatory Diseases

CYP2C9 Polymorphism Influence in PK/PD Model of Naproxen and 6-O-Desmethylnaproxen in Oral Fluid

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Hydroxypropyl β-Cyclodextrin-Functionalized MoO₃ Quantum Dots as a Multifunctional Probe for PGE2 Detection, Bioimaging, and Oxidative Stress Management in Inflammatory Diseases