Detection of a Short CCR5 Messenger RNA Isoform in Human Spermatozoa

Januchowski, Radosław; Bręborowicz, Andrzej; Ofori, Harold; Jȩdrzejczak, Piotr; Pawełczyk, Leszek; Jagodzińśki, Paweł P.

doi:10.1002/j.1939-4640.2004.tb02852.x

Cited by 13 publications

(9 citation statements)

References 27 publications

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“…Western blotting analysis confirmed the presence of both receptor proteins of the expected molecular weights. Our data are in agreement with the recent demonstration, by RT-PCR, that CCR5 mRNAs are present in ejaculated human spermatozoa (9,10), which represents the first evidence for the presence of CCR3 on the surface of human spermatozoa. We suggest that previous failure (26) to demonstrate significant CCR5 levels on human spermatozoa by flow cytometry, using FITC-conjugated monoclonal antibody, may have been due to technical problems.…”

Section: Discussionsupporting

confidence: 93%

“…Alfa‐chemokines (CXCs) and ß‐chemokines (CCs) are the two main subfamilies of chemokines, whose specific receptors are classified as a and ß chemokine receptors, respectively (8). More recently, it has been demonstrated that human spermatozoa contain mRNA coding for the CC‐chemokine receptors 1 and 5 (CCR1 and CCR5; refs 9, 10) that bind the ß chemokine regulated upon activation of normal T‐cells expressed and secreted (RANTES), and that RANTES has a chemotactic effect on human sperm (9). The presence of CX3CR1 mRNA and protein, a receptor for an additional subgroup of chemokines, has also been demonstrated in sperm; it has consequently been suggested that fractalkine, the corresponding physiological ligand, plays a role in maintaining sperm motility and regulating sperm chemotaxis (11).…”

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confidence: 99%

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Beta‐chemokine receptors 5 and 3 are expressed on the head region of human spermatozoon

et al. 2005

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Induction of human sperm chemotaxis is an established phenomenon, though signaling systems physiologically involved have not been identified. Recently, it has been demonstrated that RANTES is present in the follicular fluid and that this molecule is a chemoactractant for human spermatozoa. However, the presence of beta-chemokine receptors on human spermatozoa has never been reported. By cytometric, Western blotting and immunofluorescence analysis, we demonstrate the presence of CCR5 and CCR3 on ejaculated spermatozoa from healthy subjects. CCR5 was detected in the periacrosomal region of the sperm surface, whereas CCR3 was also present in the postacrosomal cap. Individual variability was observed on CCR5 and CCR3 positive sperm percentages. Presence of Delta32+/-) mutation was demonstrated in two subjects expressing CCR5 in half of the ejaculated spermatozoa. Our findings represent the missing information in favor of the possibility that beta-chemokines and their receptors are involved in sperm chemotaxis. Identification of molecular mechanisms of sperm chemotaxis may allow us to identify predictive parameters of sperm fertilizing ability in hypofertile or infertile subjects. Finally, both CCR5 and CCR3 expressed on the sperm cell surface may be involved in HIV-1 adhesion to spermatozoa, thus allowing these cells to perform as virion cellular carriers during sexual transmission of HIV-1 infection.

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Section: Discussionsupporting

confidence: 93%

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confidence: 99%

Beta‐chemokine receptors 5 and 3 are expressed on the head region of human spermatozoon

et al. 2005

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“…This RNA is organized in nonpolysomal messenger ribonucleoprotein complexes prior to exploitation during translation in round spermatids [Hecht 1990;Miller et al 1999]. A number of transcripts survive spermatid condensation and are present in human ejaculated spermatozoa [Miller et al 1999;Ostermeier et al 2002;Januchowski et al 2004;Jedrzejczak et al 2006]. These spermatozoal mRNA remnants might constitute a record of gene expression during spermatogenesis [Ostermeier et al 2004].…”

Section: Resultsmentioning

confidence: 99%

Quantitative Assessment of Transition Proteins 1, 2 Spermatid-Specific Linker Histone H1-Like Protein Transcripts in Spermatozoa from Normozoospermic and Asthenozoospermic Men

Jȩdrzejczak

Kempisty

Bryja

et al. 2007

Archives of Andrology

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Spermatid-specific linker histone H1-like protein (HILS1), transition proteins 1 and 2 (TNP1 and TNP2), and protamines 1 and 2 (PRM1 and PRM2) contribute to considerable dense packing of spermatid chromatin during spermiogenesis. We evaluated the HILS1, TNP1, and TNP2 transcript levels in spermatozoa isolated from normozoospermic and asthenozoospermic men. Human ejaculates from normozoospermic (n ¼ 70) and asthenozoospermic (n ¼ 100) donors were purified by centrifugation through a discontinuous Percoll density gradient. RNA was isolated from spermatozoa according to the Chomczyński and Sacchi method, treated with DNase I and reverse-transcribed into cDNA. Quantitative analysis of HILS1, TNP1, and TNP2 transcripts was performed by real-time quantitative (RQ-PCR) SYBR green I analysis. We found significantly lower levels of HILS1, TNP1, and TNP2 transcripts in spermatozoa from asthenozoospermic men compared to normozoospermic men. Our observations suggest that a reduction in HILS1, TNP1, and TNP2 transcripts may be associated with asthenozoospermia.

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“…On the other hand, DNA hypomethylation can be responsible for the transcription of endogenous retroviruses (HERV) (Januchowski et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Tracking microdeletions of the AZF region in a patrilineal line of infertile men

Rodovalho¹,

Arruda²,

Moura³

2008

Genet. Mol. Res.

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ABSTRACT. Male infertility is considered to be a difficult-to-treat condition because it is not a single entity, but rather reflects a variety of different pathologic conditions, thus making it difficult to use a single treatment strategy. Structural alterations in the Y chromosome have been the principal factor responsible for male infertility. We examined 26 family members of 13 patients with male infertility who showed deletions in the AZF region. In family 1, the father and a brother did not show microdeletions. However, a son showed a microdeletion in AZFa (sY84) and an azoospermic sperm analysis, but another son had a microdeletion in AZFa (sY84) and AZFb (sY127) and a normal sperm analysis. The father of family 2, with severe oligozoospermia, had a microdeletion in the AZFa region (sY84) and his son, conceived by intracytoplasmic sperm injection, also showed the same microdeletion. In the other families, only the men with an altered sperm analysis had a microdeletion. It is possible that in family 1, the father and brother who did not show microdeletions in this study, could have microdeletions in regions upstream or downstream of the one analyzed. The treatment with intracytoplasmic sperm injection can result in vertical transmission of microdeletions of the AZF region and can also cause the expansion of a de novo mutation. This finding reinforces the necessity of an

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Detection of a Short CCR5 Messenger RNA Isoform in Human Spermatozoa

Cited by 13 publications

References 27 publications

Beta‐chemokine receptors 5 and 3 are expressed on the head region of human spermatozoon

Beta‐chemokine receptors 5 and 3 are expressed on the head region of human spermatozoon

Quantitative Assessment of Transition Proteins 1, 2 Spermatid-Specific Linker Histone H1-Like Protein Transcripts in Spermatozoa from Normozoospermic and Asthenozoospermic Men

Tracking microdeletions of the AZF region in a patrilineal line of infertile men

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