Detection of ATP and creatine kinase using an enzyme electrode

Davis, Graham; Green, Monika J.; Hill, H. Allen O.

doi:10.1016/0141-0229(86)90134-1

Cited by 31 publications

(17 citation statements)

References 12 publications

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“…However, few biosensors for CK determination were described up to date. The first biosensor was proposed in 1986; it was amperometric and was based on graphite disk electrodes, and glucose oxidase-hexokinase enzyme system was used to determine the rate of ATP production by the CK [2]. This work for the first time demonstrated determination of CK by using an ATP-sensitive biosensor.…”

Section: Introductionmentioning

confidence: 95%

“…The determination of CK activity is based on the measurement of the rate of ATP production by using a biosensor that is sensitive to ATP and glucose [2]. The operation of the biosensor is based on two simultaneous enzymatic reactions competing for substrate Dglucose and the reaction of hydrogen peroxide oxidation on the working electrode [10].…”

Section: The Principle Of Biosensor Operationmentioning

confidence: 99%

“…CK concentration in child serum is several times bigger than in the case of adults. After intensive physical work and muscular injuries (because of different inflammation processes, myocardial infarction and muscular dystrophy) CK enters blood and its activity increases up to 2.0 U/ml or 39-185 ng/ml [1][2][3]. Furthermore, CK activity in blood serum increases in the case of hypothyroidism.…”

Section: Introductionmentioning

confidence: 96%

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Determination of total creatine kinase activity in blood serum using an amperometric biosensor based on glucose oxidase and hexokinase

Кучеренко

Солдаткін

Lagarde

et al. 2015

Talanta

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Section: Introductionmentioning

confidence: 95%

Section: The Principle Of Biosensor Operationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

See 1 more Smart Citation

Determination of total creatine kinase activity in blood serum using an amperometric biosensor based on glucose oxidase and hexokinase

Кучеренко

Солдаткін

Lagarde

et al. 2015

Talanta

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“…Nagy et al [11] employed a potential of 0.6 V versus Ag|AgCl with a response time of 10 min, Davis et al [12] and Toyoda et al [13] applied a potential of 0.36 V versus SCE to test the CK activity. Other materials in blood can be oxidized at high potentials, inducing inexact results.…”

Section: Introductionmentioning

confidence: 99%

A novel disposable amperometric biosensor based on trienzyme electrode for the determination of total creatine kinase

Liu

Jiang

Wang

et al. 2007

Sensors and Actuators B: Chemical

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“…In this way assays for adenosine triphosphate and creatine kinase have been developed by coupling through hexokinase [75].…”

Section: Analytical Systems Based On Mediated Electron Transfermentioning

confidence: 99%

Direct and indirect electron transfer between electrodes and redox proteins

Frew¹,

Hill

1988

European Journal of Biochemistry

298

118

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The direct electrochemistry of redox proteins has been achieved at a variety of electrodes, including modified gold, pyrolytic graphite and metal oxides. Careful design of electrode surfaces and electrolyte conditions are required for the attainment of rapid and reversible protein-electrode interaction. The electron transfer reactions of more complex systems, such as redox enzymes, are now being examined. The 'well-behaved' electrochemistry of redox proteins can be usefully exploited by coupling the electrode reaction to enzymes for which the redox proteins act as cofactors. In systems where direct electron transfer is very slow, small electron carriers, or mediators, may be employed to enhance the rate of electron exchange with the electrode. The organometallic compound ferrocene and its derivatives have proved particularly effective in this role. A new generation of electrochemical biosensors employs ferrocene derivatives as mediators.Many of the fundamental processes in Nature rely upon the redox processes of constituent biomolecules. Cell respiration involves the stepwise oxidation of organic substrates via a cascade of redox reactions. In photosynthetic organisms, chains of electron carriers, for example, flavoproteins, ironsulphur proteins and cytochromes, participate in light-induced photosynthetic electron transport. The intrinsic mechanisms of electron transfer reactions, and the detailed role of the protein in aiding the electron transfer between redox centres, have become foci for extensive physical and biochemical investigations [l].Electrochemistry provides a powerful tool for examining electron transfer properties. The molecules of particular interest are redox-active proteins and enzymes. Although there have been many investigations of the electrochemical behaviour of the isolated and 'free' prosthetic groups [2, 31, the properties of the latter in an intact protein are so perturbed that to gain an understanding of their biological role, it is essential to have information on the behaviour of the proteins themselves.Only relatively recently has the feasibility of studying the electron transfer reactions of redox proteins at an electrode surface been viewed with any real optimism. Indeed, for many years it was thought that reversible, direct electron transfer (i. e. without mediation by small electron carriers) between electrodes and redox proteins was not possible. Several reasons were given: the slow rates of diffusion of these species were presumed to lead to greatly diminished faradaic currents; the 'buried' nature of the redox groups would make interaction with an electrode prohibitive for all but a few orientations of the protein at the electrode surface and proteins appeared to denature at the electrode-electrolyte interface. In fact, the early studies of protein electrochemistry were unlikely to succeed simply due to the type of electrodes used [4-61. Mercury was the favoured electrode material and, in such systems, adsorption phenomena play a predominant role with the possibility of confor...

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Detection of ATP and creatine kinase using an enzyme electrode

Cited by 31 publications

References 12 publications

Determination of total creatine kinase activity in blood serum using an amperometric biosensor based on glucose oxidase and hexokinase

Determination of total creatine kinase activity in blood serum using an amperometric biosensor based on glucose oxidase and hexokinase

A novel disposable amperometric biosensor based on trienzyme electrode for the determination of total creatine kinase

Direct and indirect electron transfer between electrodes and redox proteins

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