Detection of biofilm formation in bacteria from cases of urinary tract infections, septicemia, skin and soft tissue infections and post-operative infections by Congo Red Agar method

De, Ankan; Deshpande, Dhanashree; Baveja, Sujata; Taklikar, Shripad

doi:10.4103/2249-4855.104017

Cited by 2 publications

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“…In addition, tissue Culture Plate (TCP) method was used to determine the degree of biofilm production (strong, intermediate and weak). The results confirmed the previous findings by Congo red method in which L. acidophilus HT1 had no capability to produce biofilm and these results were in general in agreement with the findings observed by Hassan et al, (17) and Anuradha et al, (3). The negative formation of biofilm by the selected L. acidophilus HT1 provides an evidence for the biosafety usage as probiotic.…”

Section: Resultssupporting

confidence: 92%

A PROBIOTIC APPLICATION OF Lactobacillus acidophilus HT1 FOR THE TREATMENT OF SOME SKIN PATHOGENS

Rasheed¹

2020

Iraqi J. Agric. Sci.

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The present study was designed to investigate the possibility of using Lactobacillus as a skin probiotic. For this purpose, 63 isolates of Lactobacillus sp. were collected from dairy products and human mouth samples. The isolates were screened for bacteriocin production by primary and secondary screening against three common skin pathogens. The results showed that the isolate Lactobacillus HT1give the highest productivity of bacteriocin (320AU/ml), the efficient isolate was identified through molecular detection using 16S ribosomal RNA gene. In addition, result showed that L. acidophilus HT1 was un encapsulated; has no capability to produce biofilm and was susceptible to amoxicillin–clavulanic acid, vancomycin and chloramphenicol. Moreover, the infectious dose of L. acidophilus HT1 was studied and results revealed that mice were remained in a healthy condition including all vital processes such as breathing, consuming food and water as well as external and anatomical appearance. Next, an emul gel formula contained L. acidophilus HT1 biomass was prepared which already examined in vitro and in vivo. Our results confirmed the efficacy of the formula via inhibiting the growth of pathogenic isolates and through several points included: viability of L. acidophilus HT1 cells, its ability to produce bacteriocin, and its releasing from the formula to the external environment without trapping or interaction among bacteriocin and any component of formula. In vivo results approved the affectivity of L. acidophilus HT1 biomass to treat the wounds infected with different bacterial pathogens during seven days after the onset of treatment compared with the control groups.

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Section: Resultssupporting

confidence: 92%

A PROBIOTIC APPLICATION OF Lactobacillus acidophilus HT1 FOR THE TREATMENT OF SOME SKIN PATHOGENS

Rasheed¹

2020

Iraqi J. Agric. Sci.

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Biofilm Formation and its Association with Gram Negative Sepsis Pathogenicity

Juliana

Leela

Gopinathan

et al. 2022

Biomed. Pharmacol. J.

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Background and objectives: Biofilm formation is an important virulence factor that protects an organism from antimicrobial agents as well as host immune effectors, thus allowing organisms to invade, survive, and cause persistent-reoccurring infection in host cells. The aim of this study was to investigate the ability of sepsis-causing gram-negative bacteria to form biofilms, evaluate the association between antibiotic resistance pattern and biofilm formation, determine the role and influence of biofilm formation on pathogenicity and clinical outcome of sepsis. Methods: A prospective study conducted from October 2020 to August 2021, non-replicated gram-negative bacteria isolates were recovered from blood samples of patients with suspected bacteremia, sepsis, and sepsis shock and identified using biochemical procedures. Antimicrobial susceptibility patterns of GNB isolates were determined using the Kirby-Bauer disc diffusion method and interpreted using CLSI guidelines. The ability of GNB isolates to form biofilm was assessed using Congo red agar and the tissue culture plate method. Results: Of the 160 Gram-negative bacteria tested, biofilm formation was seen in 73 (45.63%) isolates. Isolates are Klebsiella pneumoniae (39.73%), Acinetobacter spp. (34.25%), Escherichia coli (23.29%), Pseudomonas aeruginosa (1.37%), and other non-fermenters (1.37%). Isolates were highly resistant to cephalosporins, fluoroquinolones, and the penicillin group of antibiotics. No statistical relationship was found between resistance pattern, clinical outcome, and biofilm formation. Conclusion: In the current study, we found that 45.63% of gram-negative bacteria causing sepsis were biofilm producers. Klebsiella pneumonia isolates exhibited the highest levels of biofilm formation and antimicrobial resistance. Based on the strength of biofilm formation, most isolates were weak biofilm producers, and there was no statistical correlation between the formation of biofilms and antimicrobial resistance, indicating that the formation of biofilms was not a determining factor for resistance.

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Detection of biofilm formation in bacteria from cases of urinary tract infections, septicemia, skin and soft tissue infections and post-operative infections by Congo Red Agar method

Abstract: more prevalent in MRSA (38.46%), followed by E. coli and K. pneumoniae (15.39% each). In post-op. infections, only one

Cited by 2 publications

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A PROBIOTIC APPLICATION OF Lactobacillus acidophilus HT1 FOR THE TREATMENT OF SOME SKIN PATHOGENS

A PROBIOTIC APPLICATION OF Lactobacillus acidophilus HT1 FOR THE TREATMENT OF SOME SKIN PATHOGENS

Biofilm Formation and its Association with Gram Negative Sepsis Pathogenicity

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