Detection of bovine torovirus in fecal specimens from calves with diarrhea in Turkey

Gülaçti, İrem; Işidan, Hakan; Sözdutmaz, İbrahim

doi:10.1007/s00705-014-1977-7

Cited by 16 publications

(13 citation statements)

References 28 publications

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“…The percentage of BToV infection found in this study (1.74%) is comparatively lower than that reported in other studies: 2.9% in South Korea [ 22 ], 4.7% in Turkey [ 24 ], 5.2% in Austria [ 19 ], 6.25% in Brazil [ 23 ], 8.4% in Japan [ 21 ], 9.7% in the USA [ 18 ], 36.4% in Canada [ 4 ], and 43.2% in Croatia [ 26 ]. Despite these differences, one cannot make a reliable comparison among these percentages because of the limited sample size used in the present and previous studies.…”

Section: Discussioncontrasting

confidence: 85%

First report and genetic characterization of bovine torovirus in diarrhoeic calves in China

et al. 2020

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Background: Coronaviruses are notorious pathogens that cause diarrheic and respiratory diseases in humans and animals. Although the epidemiology and pathogenicity of coronaviruses have gained substantial attention, little is known about bovine coronavirus in cattle, which possesses a close relationship with human coronavirus. Bovine torovirus (BToV) is a newly identified relevant pathogen associated with cattle diarrhoea and respiratory diseases, and its epidemiology in the Chinese cattle industry remains unknown. Results: In this study, a total of 461 diarrhoeic faecal samples were collected from 38 different farms in three intensive cattle farming regions and analysed. Our results demonstrated that BToV is present in China, with a low prevalence rate of 1.74% (8/461). The full-length spike genes were further cloned from eight clinical samples (five farms in Henan Province). Phylogenetic analysis showed that two different subclades of BToV strains are circulating in China. Meanwhile, the three BToV strains identified from dairy calves, 18,307, 2YY and 5YY, all contained the amino acid variants R614Q, I801T, N841S and Q885E. Conclusions: This is the first report to confirm the presence of BToV in beef and dairy calves in China with diarrhea, which extend our understanding of the epidemiology of BToVs worldwide.

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Section: Discussioncontrasting

confidence: 85%

First report and genetic characterization of bovine torovirus in diarrhoeic calves in China

et al. 2020

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“…Rotavirus is the main viral causative agent of diarrhea in calves worldwide [6]. However, several other viruses, including coronavirus, bovine viral diarrhea virus (BVDV), adenovirus, kobuvirus, norovirus, parvovirus, and torovirus, have been recognized as the causative agents of diarrhea [378910111213]. The major causative agents of bacterial diarrhea are Escherichia coli , Clostridium species (spp.…”

Section: Introductionmentioning

confidence: 99%

Causative agents and epidemiology of diarrhea in Korean native calves

et al. 2019

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Calf diarrhea caused by infectious agents is associated with economic losses in the cattle industry. The purpose of this study was to identify the causative agents and epidemiological characteristics of diarrhea in Korean native calves (KNC). In total, 207 diarrheal KNC aged less than 7 months were investigated. Fecal samples collected from the rectum were examined for causative agents using polymerase chain reaction (PCR) or real-time PCR and the number of oocysts were counted. Fourteen causative agents were detected from 164 of the 207 diarrheal KNC. Rotavirus was the most common agent (34.8%), followed by Eimeria spp. (31.7%), Escherichia coli (22.0%), Giardia spp. (14.0%), Clostridium difficile (9.8%), bovine viral diarrhea virus (8.5%), coronavirus (7.9%), Cryptosporidium spp. (7.3%), torovirus (6.7%), parvovirus (5.5%), norovirus (4.9%), kobuvirus (1.8%), adenovirus (1.2%), and Salmonella spp. (0.6%). About 95 (57.9%) of 164 calves were infected with a single causative agent and 42.1% were infected by multiple agents. No significant difference was observed in mortality between calves infected with a single agent and multiple agents. The occurrence of diarrhea caused by rotavirus, Eimeria spp., kobuvirus, and Giardia spp. was significantly different based on onset age, and the prevalence of diarrhea caused by rotavirus or C. difficile was significantly different between seasons. This study help the understanding of KNC diarrhea for the development of an effective strategy for disease prevention and control, especially in Eastern provinces of South Korea.

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“…Based on the HE gene sequences, BTOV can be divided into three genotypes [18]. In this study, a phylogenetic tree constructed using all 18 complete HE gene sequences available in the GenBank database showed that the two HE sequences belonged to a genotype that is prevalent throughout the world [10,12,25,37]. The torovirus HE protein contains three domains: MP, E and R, among which the R domain is involved in receptor recognition and plays an important role in the process of BToV infection [38].…”

Section: Discussionmentioning

confidence: 86%

“…The virus can not only be detected in feces but also in the respiratory tract, indicating that the virus has dual tissue tropism [6][7][8][9]. BToV has been detected in 16 countries with a wide geographical distribution [2,7,[10][11][12][13][14][15][16][17][18][19]. In addition, a sequence reported to be from goat torovirus is present in the GenBank database, and in 2012, researchers detected the presence of PToV in pig herds by RT-PCR in China [20].…”

Section: Introductionmentioning

confidence: 99%

First detection and genomic characteristics of bovine torovirus in dairy calves in China

Zhang

Yue

et al. 2020

Arch Virol

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Bovine torovirus (BToV) is a diarrhea-causing pathogen. In this study, 92 diarrheic fecal samples from five farms in four provinces in China were collected and tested for BToV using a RT-PCR assay, and 21.73% samples were found to be BToV positive. Moreover, two complete BToV genome sequences (MN073058 and MN073059) were obtained from the clinical samples, which were 28,297 and 28,301 nucleotides in length, respectively. Sequence analysis showed that the two isolates shared 10 identical amino acid mutations in the S protein compared to the complete S sequences of BToV available in the GenBank database. In addition, seven consecutive amino acid mutations were found from aa 1,486 to 1,492 in the S protein of isolate MN073058. Moreover, the two isolates shared one identical amino acid mutation in the receptor binding sites of the HE protein. To the best of our knowledge, this is the first report on the epidemic and genomic characterization of BToV in China, which is helpful for further understanding the genetic evolution of BToV.

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Detection of bovine torovirus in fecal specimens from calves with diarrhea in Turkey

Cited by 16 publications

References 28 publications

First report and genetic characterization of bovine torovirus in diarrhoeic calves in China

First report and genetic characterization of bovine torovirus in diarrhoeic calves in China

Causative agents and epidemiology of diarrhea in Korean native calves

First detection and genomic characteristics of bovine torovirus in dairy calves in China

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