Detection of bovine whey proteins by on-column derivatization capillary electrophoresis with laser-induced fluorescence monitoring

Benito, I.; Marina, Marı́a Luisa; Saz, J.M.; Diez-Masa, Jose Carlos

doi:10.1016/s0021-9673(99)00272-1

Cited by 41 publications

(19 citation statements)

References 30 publications

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“…The principle is based on the enhancement of the quantum yield of these dyes when they are associated to specific sites of some proteins, yielding a blue shift in the emission wavelength with an intensity which depends on the protein and separation conditions [169]. The performances of N-arylaminonaphthalene sulfonate dyes such as 1-anilinonaphthalene-8-sulfonic acid (ANS), 4,4'-dianilino-5,5'-disulfonic acid (bis-ANS) and 2-(p-toluidino) naphthalene-6-sulfonic acid (2,6-TNS) were evaluated as precolumn derivatization reagents for the detection of bovine whey proteins using CE-LIF [170]. The best results were obtained using 2,6-TNS dye and 2-(N-3.3.3.cyclohexylamino) with ethanesulfonic acid (CHES) pH 10.2 as electrolyte (Fig.…”

Section: N-arylaminonaphthalene Sulfonate Compoundsmentioning

confidence: 99%

LIF detection of peptides and proteins in CE

2007

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CE- and microchip-based separations coupled with LIF are powerful tools for the separation, detection and determination of biomolecules. CE with certain configurations has the potential to detect a small number of molecules or even a single molecule, thanks to the high spatial coherence of the laser source which permits the excitation of very small sample volumes with high efficiency. This review article discusses the use of LIF detection for the analysis of peptides and proteins in CE. The most common laser sources, basic instrumentation, derivatization modes and set-ups are briefly presented and special attention is paid to the different fluorogenic agents used for pre-, on- and postcapillary derivatization of the functional groups of these compounds. A table summarizing major applications of these derivatization reactions to the analysis of peptides and proteins in CE-LIF and a bibliography with 184 references are provided which covers papers published to the end of 2005.

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Section: N-arylaminonaphthalene Sulfonate Compoundsmentioning

confidence: 99%

LIF detection of peptides and proteins in CE

2007

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“…Benito et al [59] developed an on-capillary derivatization, to determine some bovine whey proteins in raw milk samples. Although the N-arylaminonaphthalene sulfonate derivatives used have sufficient sensivity for UV detection at the concentrations present in milk samples, a fluorescent probe based on LIF was used because of fear of other interfering substances with UV activity which are present in the milk samples.…”

Section: Throughout Techniquementioning

confidence: 99%

Advances in capillary electrophoretically mediated microanalysis

et al. 2003

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Recent developments in the use of capillary electrophoretic techniques for the in-line study of enzyme reactions and derivatization protocols are reviewed. The article is divided into two parts: (i) in-line enzyme reactions and (ii) in-line derivatization. The first part introduces electrophoretically mediated microanalysis (EMMA) and discusses and illustrates the different modes of EMMA. A literature overview is provided, starting from 1996, and the investigated enzymes are classified into two tables based on the mode of engagement (i.e., continuous or transient) of the developed EMMA-based assay. The second part starts with an introduction of the procedures and the nomenclature used in the area of in-line derivatization protocols based on EMMA. Reported derivatization procedures are discussed and classified in tables, according to the functional group that is derivatized.

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“…CE has become an important tool in proteomics and genomics. The use of CE for analysis of proteins has been reviewed until 2001 [60][61][62], peptides until 1999 [63], and DNA up to 2003 [64]. This section is concentrated on new contributions to technique development, sample pretreatment, column technologies, improvement in resolution and detection, and special techniques.…”

Section: General Aspectsmentioning

confidence: 99%

“…Fluorescent agents, associating with a protein but are not covalently bound, gained significant popularity [14,15]. These dyes have a strong fluorescence when associated with a protein, but only a very slight fluorescence when free in solution [73,74]. Furthermore, on-column derivatization with phthalic anhydride by differential mobility was studied [75].…”

Section: Derivatizationmentioning

confidence: 99%

Recent progress in high‐performance capillary bioseparations

et al. 2003

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Bioanalysis is a fast growing domain with new technological developments in the field of mass spectrometry, separation science, and bioinformatics. The central role of separation prior to detection and evaluation of analytes is indisputable. In fact, since the decipherment of the human genome via multichannel capillary electrophoresis in combination with laser-induced fluorescence [1], the analysis of the proteome, i.e., the type and amount of all proteins expressed at a certain time by the genome in a cell or tissue [2] became of central interest. Therefore, new fast and sensitive capillary liquid chromatographic, electrophoretic, and electrochromatographic separation techniques, suitable for high-throughput analysis of proteins, peptides and nucleic acids, are required.

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Detection of bovine whey proteins by on-column derivatization capillary electrophoresis with laser-induced fluorescence monitoring

Cited by 41 publications

References 30 publications

LIF detection of peptides and proteins in CE

LIF detection of peptides and proteins in CE

Advances in capillary electrophoretically mediated microanalysis

Recent progress in high‐performance capillary bioseparations

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