Detection of Canine Herpesvirus 1 in a wide range of tissues using the polymerase chain reaction

Burr, Paul; Campbell, Moyra E.M.; Nicolson, Lesley; Onions, David

doi:10.1016/s0378-1135(96)01227-8

Cited by 72 publications

(66 citation statements)

References 23 publications

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“…Although no virus was recovered from vaginal swabs of these animals, CHV must replicate in the epithelial cells of vagina and be transported to the ganglia. It was suggested that the lumbosacral ganglion was an important site for latency and that recrudescent virus played an important role in venereal infections [4]. Since CHV infection in pregnant dogs causes abortion and stillbirth, the mechanism of reactivation of CHV should be clarified [9,15].…”

Section: Discussionmentioning

confidence: 99%

“…The nested PCR was performed in a 25 µl volume containing 1 µl diluted PCR products, 50 µM IE-1N primer (5'-AACCAACTCCAGCTAAAGCAT-3'), 50 µM IE-2N Canine herpesvirus (CHV) causes a generalized necrotizing and hemorrhagic disease in neonatal pups, and usually subclinical upper respiratory infection in adult dogs [1]. It has been indicated that CHV persists in the trigeminal ganglion or a variety of tissues of naturally infected dogs as viral DNA, and that stress or administration of corticosteroid leads reactivation of the virus [4,14]. CHV belongs to the alphaherpesvirus group, containing feline herpesvirus 1 (FHV-1), equine herpesvirus 1 (EHV-1), bovine herpesvirus 1 (BHV-1) and pseudorabies virus (PRV) [18].…”

Section: 7 and 8)mentioning

confidence: 99%

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Detection of Canine Herpesvirus DNA in the Ganglionic Neurons and the Lymph Node Lymphocytes of Latently Infected Dogs.

et al. 1999

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ABSTRACT. To determine the site of latent infection of canine herpesvirus (CHV), tissues from dogs convalescent from acute infection with CHV were examined for the presence of viral genome DNA by the nested polymerase chain reaction. CHV DNA was detected in the trigeminal ganglia and the retropharyngeal lymph nodes. In situ hybridization study of the tissues revealed that CHV genome persisted in the nuclei of ganglionic neurons and lymphocytes.-KEY WORDS: canine herpesvirus, ganglionic neuron, latency, lymphocyte.

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Section: Discussionmentioning

confidence: 99%

Section: 7 and 8)mentioning

confidence: 99%

Detection of Canine Herpesvirus DNA in the Ganglionic Neurons and the Lymph Node Lymphocytes of Latently Infected Dogs.

et al. 1999

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“…Lesions in the vestibulum and vagina of bitches may recur when bitches come into proestrus and regress when they go into anestrus [8]. Affected animals may remain latent carriers [10], and the virus was identified in the lumbosacral ganglia, tonsils, parotid salivary glands and liver of dogs that showed no sign of herpesvirus infection [11].…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of canine herpesvirus in breeding kennels in the Gauteng Province of South Africa

et al. 2008

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Canine herpesvirus (CHV-1) causes neonatal deaths as well as infertility due to embryonal death, abortion and stillbirths in breeding kennels. The aim of this study was to determine the prevalence of antibodies against canine herpesvirus in the serum of dogs older than 1 year in breeding kennels in the Gauteng Province of South Africa. A serum neutralization test (SNT) and a newly developed enzyme linked immunosorbent assay (ELISA) were used to test the serum samples of 328 dogs in 38 breeding kennels. With SNT as well as ELISA, 22% of sera were positive (P > 0.9). Seventeen kennels (45% of total kennels) each had at least one positive dog on SNT compared with twenty kennels (53% of total kennels) that each had at least one positive dog on ELISA (P = 0.6). The prevalence of positive dogs in positive kennels was 42 ± 26% (n = 17 kennels) for SNT and 39 ± 26% (n = 20 kennels) for ELISA. Pairwise comparison of kennels showed that the prevalence of SNT positive dogs was similar to the prevalence of ELISA positive dogs (P = 0.3, n = 38 kennels). Seroprevalence was independent of age, gender or colony size. This study suggests that canine herpesvirus is sufficiently common in breeding dogs in the Gauteng Province of South Africa to pose a threat to neonatal survival and fertility.

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“…The other protocol directly amplified a 120-bp fragment from the glycoprotein B gene. The last protocol used one forward (P1: 5 'CAG GACTATTGGACTATAGT3') and one reverse (5 'TTG CAATGCCCCTCATAATT3') primer [11].…”

Section: Methodsmentioning

confidence: 99%

Pathology Isolation and Identification of Canine Herpesvirus (CHV-1) in Mexico

Lara¹,

Romero²,

Marín³

et al. 2016

OJPathology

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This work presents the pathology description, isolation and identification of canine herpesvirus (CHV-1) in Mexico, a virus that causes a generalized hemorrhagic infection in puppies from the canidae family. Methods: Isolates were obtained from puppies that died within the first four weeks of life and had lesions consistent with canine herpesvirus. Results: The main gross lesions were petechial and ecchymotic hemorrhages in kidneys, liver and lungs; proliferative interstitial nephritis; multifocal necrosis in liver and kidneys; and encephalitis with intranuclear inclusion bodies. Herpesvirus was confirmed through direct immunofluorescence, electron microscopy and polymerase chain reaction for DNA polymerase and glycoprotein B genes. Discussion: Eight strains were isolated and identified as canine herpesvirus corresponding to three of the working cases with gross and microscopic lesions very similar to those described in the literature; then, isolates were confirmed by PCR gene amplification, positive reactions on immunofluorescence and observations from electron microscopy. This work represents the first report of this disease, including gross and histological lesions, and confirmation by isolation and identification of the canine herpesvirus in Mexico.

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Detection of Canine Herpesvirus 1 in a wide range of tissues using the polymerase chain reaction

Cited by 72 publications

References 23 publications

Detection of Canine Herpesvirus DNA in the Ganglionic Neurons and the Lymph Node Lymphocytes of Latently Infected Dogs.

Detection of Canine Herpesvirus DNA in the Ganglionic Neurons and the Lymph Node Lymphocytes of Latently Infected Dogs.

Seroprevalence of canine herpesvirus in breeding kennels in the Gauteng Province of South Africa

Pathology Isolation and Identification of Canine Herpesvirus (CHV-1) in Mexico

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