Detection of carbohydrate terminals in the enteric parasite Enteromyxum scophthalmi (Myxozoa) and possible interactions with its fish host Psetta maxima

Redondo, María J.; Cortadellas, Nuria; Palenzuela, Oswaldo; Álvarez-Pellitero, Pilar

doi:10.1007/s00436-008-0903-x

Cited by 26 publications

(32 citation statements)

References 43 publications

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“…The detected glycoproteic antigens of 15, 34 and 165 kDa of Enteromyxum leei contained Man/Glc moieties, which were the most abundant monosaccharides among the parasite's antigens, compared to the other monosaccharides tested. This result agrees with the ConA staining of E. leei proliferative and sporogonic stages observed by light and electron microscopy (Redondo & Álvarez-Pellitero 2009) and is a common trait among myxozoans (Muñoz et al 1999a, 2000b, Kaltner et al 2007, Redondo et al 2008. In fact, Man/ Glc moieties were present at the host−parasite interface in enteromyxoses (Redondo et al 2008, Redondo & Álvarez-Pellitero 2009.…”

Section: Discussionsupporting

confidence: 91%

“…This result agrees with the ConA staining of E. leei proliferative and sporogonic stages observed by light and electron microscopy (Redondo & Álvarez-Pellitero 2009) and is a common trait among myxozoans (Muñoz et al 1999a, 2000b, Kaltner et al 2007, Redondo et al 2008. In fact, Man/ Glc moieties were present at the host−parasite interface in enteromyxoses (Redondo et al 2008, Redondo & Álvarez-Pellitero 2009. The presence of surface Man moieties on offending microorganisms can trigger an innate immune response via complement system by the lectin pathway or via phagocytosis by Man-binding receptors on macrophages (Kaltner et al 2007).…”

Section: Discussionsupporting

confidence: 90%

“…In accordance with this, the 165 kDa glycoproteic band of E. leei was also labelled by BSL I and SBA, both of them only binding to spores of E. leei in light microscopy preparations (Redondo & Álvarez-Pellitero 2009). These lectins recognize Gal and GalNac carbohydrate residues, which are frequently involved in host−parasite interactions and have also been detected in spores of several myxozoans (Muñoz et al 1999a, 2000b, Kaltner et al 2007, Redondo et al 2008) and in antigenic bands of Myxobolus cerebralis (Knaus & El-Matbouli 2005). The 165 kDa carbohydrate-rich antigen was not detected by PAS staining, but primary cells, secondary cells and polar capsules of E. leei were PAS positive in histological sections .…”

Section: Discussionsupporting

confidence: 62%

“…Thus, bacterial chitinases degrading spore valves of the myxozoan Thelohanellus kitauei have been suggested as alternative biological agents to control myxozoan diseases (Liu et al 2011). Interestingly, polar capsules of Enteromyxum leei were negative for WGA while other spore structures were positive and putatively contained chitin, as was also the case with the spores of the congener E. scophthalmi (Redondo et al 2008, Redondo & Álvarez-Pellitero 2009. Since these authors detected no GlcNac in proliferative stages of E. leei, we can hypothesize that the high-MW glycoprotein of 165 kDa is located in spores, and could have a structural, protective function as described for chitin in polar capsule walls.…”

Section: Discussionmentioning

confidence: 83%

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Antigenic characterization of Enteromyxum leei (Myxozoa: Myxosporea)

Estensoro¹,

Álvarez-Pellitero²,

Sitjà‐Bobadilla³

2013

Dis. Aquat. Org.

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Enteromyxum leei, an intestinal myxozoan parasite affecting a wide range of fish, was partially purified, and the immunogenic composition of its glycoproteins as well as the proteolytic activity were studied. Parasite extracts, mainly containing spores, were separated by SDS-PAGE, and thereafter, immunoblots were carried out with a polyclonal antiserum (Pab) raised against E. leei. Periodic acid/Schiff staining of gels, periodate- and Proteinase K-treated Western blots and Lectin blots were performed to analyse the terminal carbohydrate composition of the parasite's antigens. Additionally, the cross-reaction of the parasite extracts with a Pab raised against the polar filament of the myxozoan Myxobolus pendula was studied. Both Pabs detected proteic epitopes on antigenic proteins and glycoproteins of E. leei, ranging between 15 and 280 kDa. In particular, 2 glycoproteic bands (15 and 165 kDa), immunoreactive to both Pabs and with glucose and mannose moieties, could correspond to common antigens shared among myxozoans. The 165 kDa band also presented galactose, N-acetyl-galactosamine and N-acetyl-glucosamine, pointing to its possible origin on chitin-built spore valves and to its possible involvement in host-parasite interactions. The molecular weight of the 15 kDa glycoproteic antigen matches that of minicollagen, a cnidarian-specific protein of nematocysts with a myxozoan homologue. Several proteases with apparent molecular weights ranging between 43 and 245 kDa were found in zymographies of E. leei extracts, and these may have a potential role in the parasite's pathogenesis. This is a useful approach for further studies to detect targets for antiparasitic therapy.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 83%

See 2 more Smart Citations

Antigenic characterization of Enteromyxum leei (Myxozoa: Myxosporea)

Estensoro¹,

Álvarez-Pellitero²,

Sitjà‐Bobadilla³

2013

Dis. Aquat. Org.

View full text Add to dashboard Cite

show abstract

“…The current study is focused on the carbohydrate features of the mucus layer of the intestinal tract because the previous information on Enteromyxum scophthalmi and E. leei suggests a role of some carbohydrate moieties in the interaction with their hosts (Redondo et al 2008, Redondo & Alvarez-Pellitero 2009. The mucosal surface of the gastrointestinal tract is a complex organisation of epithelium, immune cells and resident microbiota (McCracken & Lorenz 2001, Rombout et al 2011.…”

Section: Introductionmentioning

confidence: 99%