Detection of E.coli, Salmonella spp., and Listeria Monocytogenes in Retail Chicken Meat and Chicken Giblets Samples Using Multiplex PCR in Baghdad City

AL-Jobori, Kamil M.; Hasan, Mareh L. Mohammed; Nader, Mohammed I.

doi:10.20546/ijcmas.2016.509.033

Cited by 3 publications

(1 citation statement)

References 32 publications

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“…According to statistics, Salmonella often tops the list of reported cases of bacterial food poisoning in countries around the world each year [11] [12]. Therefore, the detection of Salmonella is of essential significance [13] [14] [15]. In some countries, including the United States of America (USA) and the European Union (EU), kinds of regulations are established to make sure that Salmonella existing in ready-to-eat food could be detected so that consumers will not take them in [16] [17] [18].…”

Section: Introductionmentioning

confidence: 99%

Biosensor Detection Methods for Salmonella: a Review

Du¹

2023

AETR

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Salmonella is a pathogenic bacterium capable of causing serious food-borne illness, which has caused worldwide concern. It can enter the food supply chain at various stages of production, distribution, and marketing. Due to its high prevalence, it is necessary to develop effective and efficient methods for the identification, detection, and monitoring of Salmonella at an early stage. While traditional detection technologies often have long detection times, complex operation and low sensitivity, emerging biosensor technologies combine novel nanomaterials with signal amplification methods to achieve improved performance. Biosensors show several advantages over traditional assays in terms of specificity, accuracy, and sensitivity and show superiority in rapid response. They are now seen as promising alternative tools since they are accessible and applicable approaches for rapid detection. In recent years, there has been a boom in the development of biosensors for the detection of foodborne Salmonella. This paper focuses on the comparison of new methods for the detection of optical and electrochemical biosensors in recent years, analyzes the different separation strategies and determination methods used, and provides an outlook on the future development of biosensors for the detection of Salmonella.

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Section: Introductionmentioning

confidence: 99%

Biosensor Detection Methods for Salmonella: a Review

Du¹

2023

AETR

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Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samples

Ledlod

Areekit

Santiwatanakul

et al. 2020

Food sci. technol. int.

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In this study, we successfully developed a simple and rapid method for simultaneous detection of Salmonella spp., Listeria monocytogenes, and Escherichia coli using gold nanoparticles and the aptamer aptasensor. We screened 25 specific DNA aptamer candidates against these pathogens using whole-cell Systematic Evolution of Ligands by EXponential enrichment. Among them, Ap6 was selected due to its low energy minimization values of −12.25 and −27.67 kcal/mol derived from MFold and RNAFold analysis, respectively. The assay presented in this study allowed the visual colorimetric detection of labeled colloidal gold nanoparticles as well as determination of UV absorbance at 625 and 525 nm under optimized conditions. The detection limit of this aptasensor was as less as 105 CFU/ml. A random investigation of 50 meat samples, including ham and chicken sausages, collected from the local market revealed 96% accuracy, 96% specificity, and 100% sensitivity of the assay. The colorimetric aptasensor can accomplish one-step detection without pre-culture, DNA extraction, and amplification. Hence, it is an easy, rapid, specific, and qualitative assay that can be used as a point-of-care testing to directly detect multiplex foodborne pathogens.

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Safety achievement and shelf-life prolongation of poultry breast meats by polylactic acid active packaging and gamma-irradiation

Abdel-Khalek¹,

El-Aziz²,

Ibrahim³

2022

IFRJ

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Active packaging incorporated with volatile oils is a promising technology to extend the shelf-life of perishable food. Therefore, the present work aimed at producing composite pouches based on polylactic acid incorporated with a mixture of lemongrass and cumin essential oils (PLA/mix oil). The effect on the shelf-life of fresh poultry breasts was determined on samples packaged in the PLA/mix oil alone and in combination with gamma-irradiation, and stored under refrigeration through microbiological, physicochemical, and sensorial analyses. The effect of active packaging and gamma-irradiation on artificially inoculated foodborne bacteria (Escherichia coli O157:H7 ATCC 25922, Salmonella Enteritidis, Listeria monocytogenes ATCC 35152) in poultry breasts was evaluated. When compared to control, poultry breast samples packaged in the PLA/mix oil and irradiated at 4 kGy alone decreased microbial count, maintained colour and pH values, and increased TBARS index at a lower rate, thus extended the shelf-life by 21 and 14 d, respectively. However, the combination of PLA/mix oil and gamma-irradiation at 2 kGy (PLA/mix oil + 2 kGy) was more effective in decreasing all microbial counts and extending the shelf-life by more than 28 d. Initial load of S. Enteritidis, E. coli, and L. monocytogenes inoculated in poultry breasts decreased by 3.03, 2.98, and 3.19 log CFU/g, respectively, after 3 d of storage in PLA/mix oil packaging, while the combination between PLA/mix oil and gamma-irradiation at 2 kGy (PLA/mix oil + 2 kGy) caused a synergistic impact with an increase in radiosensitivity of S. Enteritidis, E. coli, and L. monocytogenes by 3.53, 4.47, and 4.23 log CFU/g, respectively, after one day of storage as compared to the control. Active packaging (PLA/mix oil) alone and in combination with gamma-irradiation can be considered an innovative technology that could have a major effect on the prolongation of shelf-life and safety of poultry breast meats. Moreover, this new technology represents a promising alternative to commercial and unsustainable plastic films.

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Detection of E.coli, Salmonella spp., and Listeria Monocytogenes in Retail Chicken Meat and Chicken Giblets Samples Using Multiplex PCR in Baghdad City

Cited by 3 publications

References 32 publications

Biosensor Detection Methods for Salmonella: a Review

Biosensor Detection Methods for Salmonella: a Review

Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samples

Safety achievement and shelf-life prolongation of poultry breast meats by polylactic acid active packaging and gamma-irradiation

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