Detection of free radicals generated from hydrogen peroxide, gallic acid and haemoprotein chemiluminescence system by electron spin resonance spectroscopy

Kawane, M.; Iida, Tetsuo; Yoshiki, Yumiko; Okubo, Kimihiro; Tsunakawa, Mitsuaki

doi:10.1002/(sici)1522-7243(199911/12)14:6<321::aid-bio568>3.0.co;2-8

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…Stolze and Nohl (8) have detected the expected phenoxyl radicals from the H 2 O 2 /metHb-phenol mixture, such as butylated hydroxyanisole, butylated hydroxytoluene and p-hydroxyanisole, unconnected with chemiluminescence. We also confirmed the production of the gallate radical (g ϭ 2.0058) from the H 2 O 2 /gallic acid/Hb system by the electron spin resonance method (22). Additionally, the gallate radical from the H 2 O 2 /gallic acid/hemoprotein system immediately decayed, and its decay ratio showed a high correlation with the photon decrease ratio.…”

Section: Discussionsupporting

confidence: 71%

Chemiluminescence of Hemoglobin and Identification of Related Compounds with the Hemoglobin Chemiluminescence in Plasma¶

Yoshiki¹,

Iida²,

Okubo³

et al. 2007

Photochemistry and Photobiology

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A low level of chemilumnescence by hemoglobin (Hb) was detected in the reaction with H2O2 and hydrogen donors such as gallic acid and catechins. The photon intensity was affected by the ferric state of Hb (methemoglobin > oxyhemoglobin), and was roughly correlated with the radical‐scavenging potential of catechins. We hypothesized the reversible activation reaction of Hb as the chemiluminescence mechanism of the H2O2/gallic acid/Hb system. It is indicated that the oxidized‐Hb (Hb‐OOH) formation was a chemiluminescence‐rate–determining step and one‐electron reduction by a hydrogen donor of the compound‐I–type intermediate ([·XFeIV]= O) proved a chemiluminescence‐specificity–determining step. Spectral analysis showed that the photon emission from the H2O2/gallic acid/Hb system was produced without singlet oxygen generation. The concentration dependence of photon intensity suggests a high consumption ratio of H2O2 leading to protection from H2O2 toxicity. Albumin was defined as a hydrogen donor by the isolation of chemiluminescent substance in plasma using this chemiluminescence system.

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Section: Discussionsupporting

confidence: 71%

Chemiluminescence of Hemoglobin and Identification of Related Compounds with the Hemoglobin Chemiluminescence in Plasma¶

Yoshiki¹,

Iida²,

Okubo³

et al. 2007

Photochemistry and Photobiology

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A Mainstay of Functional Food Science in Japan—History, Present Status, and Future Outlook

Arai

Osawa

Ohigashi

et al. 2001

Bioscience, Biotechnology, and Biochemistry

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Chemiluminescence of Hemoglobin and Identification of Related Compounds with the Hemoglobin Chemiluminescence in Plasma¶

Yoshiki

Iida

Okubo

et al. 2001

Photochem Photobiol

View full text Add to dashboard Cite

A low level of chemiluminescence by hemoglobin (Hb) was detected in the reaction with H2O2 and hydrogen donors such as gallic acid and catechins. The photon intensity was affected by the ferric state of Hb (methemoglobin > oxyhemoglobin), and was roughly correlated with the radical-scavenging potential of catechins. We hypothesized the reversible activation reaction of Hb as the chemiluminescence mechanism of the H2O2/gallic acid/Hb system. It is indicated that the oxidized-Hb (Hb-OOH) formation was a chemiluminescence-rate-determining step and one-electron reduction by a hydrogen donor of the compound-I-type intermediate ([.XFeIV] = O) proved a chemiluminescence-specificity-determining step. Spectral analysis showed that the photon emission from the H2O2/gallic acid/Hb system was produced without singlet oxygen generation. The concentration dependence of photon intensity suggests a high consumption ratio of H2O2 leading to protection from H2O2 toxicity. Albumin was defined as a hydrogen donor by the isolation of chemiluminescent substance in plasma using this chemiluminescence system.

show abstract

Detection of free radicals generated from hydrogen peroxide, gallic acid and haemoprotein chemiluminescence system by electron spin resonance spectroscopy

Cited by 3 publications

References 15 publications

Chemiluminescence of Hemoglobin and Identification of Related Compounds with the Hemoglobin Chemiluminescence in Plasma¶

Chemiluminescence of Hemoglobin and Identification of Related Compounds with the Hemoglobin Chemiluminescence in Plasma¶

A Mainstay of Functional Food Science in Japan—History, Present Status, and Future Outlook

Chemiluminescence of Hemoglobin and Identification of Related Compounds with the Hemoglobin Chemiluminescence in Plasma¶

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