Detection of HBsAg in a clone derived from the PLC/PRF/5 human hepatoma cell line

Wen, Yiping; Copeland, Jennifer A.; Mann, G. F.; Howard, Colin R.; Zuckerman, Arie J.

doi:10.1007/bf01314568

Cited by 7 publications

(2 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This cell line is a hepatoma cell line isolated from an HCC patient, and secretes a substantial amount of HBsAg that can be monitored easily. Also, HBsAg is the sole viral protein produced by PLC/PRF/5 cells (Alexander et al, 1976;Wen et al, 1981). Reduction of HBsAg secretion was observed 1 day after siRNA transfection, with maximal inhibition on the second day, consistent with previous studies (Li et al, 2004).…”

Section: Upregulation Of Lef-1 Is Associated With Shbs Expressionsupporting

confidence: 84%

Gene-expression profiles of a hepatitis B small surface antigen-secreting cell line reveal upregulation of lymphoid enhancer-binding factor 1

Tian

Zhao

Ren

et al. 2007

Journal of General Virology

View full text Add to dashboard Cite

The genome of hepatitis B virus (HBV) consists of four open reading frames, encoding the envelope proteins (Pre-S/S), the core proteins (Pre-C/C), the polymerase (P) and the transactivating X protein (X). In the sera of HBV-infected patients, hepatitis B surface antigen (HBsAg) particles without the viral genome can outnumber virions by more than 1000-fold. To analyse the interactions between HBsAg and host cells, global gene-expression profiles of a small HBsAg (SHBs)-secreting stable cell line (HepG2-S-G2) and its counterpart control cell line (HepG2-Neo-F4) were compared. Marked upregulation of lymphoid enhancer-binding factor 1 (LEF-1), a transcription factor in the Wnt pathway, was found in SHBs-expressing cells and was confirmed by interference experiments with small interfering RNA. However, compared with the control cells, HepG2-S-G2 did not show higher proliferative competence in culture or increased tumorigenesis in nude mice. A possible mechanism to explain the discrepancy between the upregulation of LEF-1 and the lack of increased tumorigenesis is SHBs expression resulting in altered expression and distribution of LEF-1 protein in cell compartments and upregulation of LEF-1 isoforms that could suppress, rather than enhance, the Wnt pathway.

show abstract

Section: Upregulation Of Lef-1 Is Associated With Shbs Expressionsupporting

confidence: 84%

Gene-expression profiles of a hepatitis B small surface antigen-secreting cell line reveal upregulation of lymphoid enhancer-binding factor 1

Tian

Zhao

Ren

et al. 2007

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…The cell line PLC/PRF/S, derived from a primary hepatocellular carcinoma, synthesizes a-fetoprotein (AFP) and hepatitis B virus surface antigen (HBsAg) among other liver specific proteins (7). The cell line has been subcloned (8). Uninfected cells were cultivated in 25-cm2 flasks (Nunc) at 37°C in minimal essential medium (MEM) supplemented with 10% fetal calf serum (FCS).…”

mentioning

confidence: 99%

Effect of Hepatitis A Virus Infection on Cell Metabolism in Vitro

Gauss‐Müller¹,

Deinhardt²

1984

Experimental Biology and Medicine

View full text Add to dashboard Cite

Hepatitis A virus (HAV), when inoculated into cultures of the PLC/PRF/5 cell line which produces the surface antigen of hepatitis B virus (HBsAg), showed growth characteristics different from those of other picornaviruses. Antigen of HAV (HAAg) is expressed only about 10 days after infection. No major impact on the overall macromolecular biosynthesis of the host cells is observed. The growth rate of HAV-infected and uninfected cells was comparable, although the plating efficiency of infected cells was lower. Different hormonal factors were tested for their ability to stimulate viral antigen expression. Dexamethasone or prostaglandin El added to the culture medium increased HAAg expression; insulin reduced expression. Persistent infection of hepatoma cells by HAV never led to a cytolytic infection. In temperature-shift experiments, an adverse effect on the expression of HAAg and HBsAg was observed. In all experiments, the amounts of HBsAg in HAV-infected cells were reduced. On the whole, no major influence on host-cell metabolism is observed in cells persistently infected with HAV. Cell-mediated immunological response as a mechanism of pathological changes in HAV-infected liver is, therefore, more likely than a cytopathological effect.

show abstract

Human Hepatoma Cell Lines

Alexander

1987

Neoplasms of the Liver

View full text Add to dashboard Cite

Detection of HBsAg in a clone derived from the PLC/PRF/5 human hepatoma cell line

Cited by 7 publications

References 8 publications

Gene-expression profiles of a hepatitis B small surface antigen-secreting cell line reveal upregulation of lymphoid enhancer-binding factor 1

Gene-expression profiles of a hepatitis B small surface antigen-secreting cell line reveal upregulation of lymphoid enhancer-binding factor 1

Effect of Hepatitis A Virus Infection on Cell Metabolism in Vitro

Human Hepatoma Cell Lines

Contact Info

Product

Resources

About