Detection of Hepatitis B Virus Large Surface Protein Using a Time‐Resolved Immunofluorometric Assay

Hu, Zhigang; Li, Mei; Liu, Jie; Lei, Yu; Xue, Yan; Chen, Yu

doi:10.1002/jcla.21800

Cited by 6 publications

(1 citation statement)

References 31 publications

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“…According to the SPR response in Figure 8, the recoveries of DENV-2 E-proteins was calculated and tabulated in Table 3. The obtained average recovery was consistent with acceptable recovery, which is in the range of 80-120%, indicates that the proposed sensor can be used for detection and quantification of DENV-2 Eproteins in real samples [92][93][94][95]. Figure 7b depicts the selective shift of SPR angle of DENV-2 E-proteins in multiple analytes solution.…”

Section: Spr Performancessupporting

confidence: 67%

An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies

et al. 2021

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This paper proposes a novel idea to enhance the sensitivity and selectivity of surface plasmon resonance (SPR) optical sensor for detection of dengue virus type-2 envelope proteins (DENV-2 E-proteins) using polyamidoamine (PAMAM) dendrimer biopolymer-based nanocomposite thin film. For this purpose, two ranges of DENV-2 E-protein concentrations, i.e., 0.000008–0.0001 nM and 0.00008–0.005 nM were evaluated, and the lowest detectable concentration was achieved at 0.00008 nM. The incorporation of PAMAM dendrimer-based nanocomposite thin film with an SPR sensor exhibited a significant increase in sensitivity and binding affinity to a lower range DENV-2 E-protein concentrations. Moreover, the proposed sensor displayed good selectivity towards DENV-2 E-proteins and have an average recovery of 80–120%. The findings of this study demonstrated that PAMAM dendrimer-based nanocomposite thin film combined with SPR sensor is a promising diagnostic tool for sensitive and selective detection of DENV-2 E-proteins.

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Section: Spr Performancessupporting

confidence: 67%

An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies

et al. 2021

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Prediction value of serum HBV large surface protein in different phases of HBV infection and virological response of chronic hepatitis B patients

Liu

Shang

et al. 2018

Clinica Chimica Acta

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New wide dynamic range assays for quantification of anti- Leishmania IgG2 and IgA antibodies in canine serum

Escribano

Bernal

Pardo-Marín

et al. 2017

Veterinary Immunology and Immunopathology

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Detection of Hepatitis B Virus Large Surface Protein Using a Time‐Resolved Immunofluorometric Assay

Abstract: The TR-IFMA that we developed for L protein presented a higher sensitivity and wider detecting range than regular commercial ELISA. Therefore, this TR-IFMA has promising value both in the screening of HBV and monitoring of antiviral therapy.

Cited by 6 publications

References 31 publications

An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies

An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies

Prediction value of serum HBV large surface protein in different phases of HBV infection and virological response of chronic hepatitis B patients

New wide dynamic range assays for quantification of anti- Leishmania IgG2 and IgA antibodies in canine serum

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