Detection of highly pathogenic avian influenza virus infection in vaccinated chicken flocks by monitoring antibodies against non-structural protein 1 (NS1)

Takeyama, Natsumi; Minari, Kenji; Kajihara, Masahiro; Isoda, Norikazu; Sakamoto, Ryuichi; Sasaki, Takashi; Kokumai, Norihide; Takikawa, Noriyasu; Shiraishi, Rikiya; Mase, Masaji; Hagiwara, Junko; Kodama, Toshiaki; Imamura, Takashi; Sakaguchi, Masashi; Ohgitani, Toshiaki; Sawata, Akira; Okamatsu, Masatoshi; Muramatsu, Masatake; Tsukamoto, Kenji; Lin, Zhifeng; Tuchiya, Kotaro; Sakoda, Yoshihiro; Kida, Hiroshi

doi:10.1016/j.vetmic.2010.07.002

Cited by 7 publications

(5 citation statements)

References 32 publications

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“…Most studies showed that NS1based tests had low accuracy as a DIVA test, as was found in this present studies (Tumpey et al 2005;Dundon & Capua 2009;Avellaneda et al 2010). Other studies, however, indicated that NS1-based ELISA ispotentially useful as DIVA test (Birch-Machin et al 1997;Ozaki et al 2001;Tumpey et al 2005;Takeyama et al 2011;Wang et al 2011;Golchinfar et al 2014). In spite of that potential of NS1-based ELISA as DIVA test, the test had rather low specificity because some vaccinated animals were also seropositive although with low antibody titres.…”

Section: Discussionsupporting

confidence: 51%

“…However, the accuracy of test based on this protein as DIVA test is still contradictory between different studies. Some studies found that the NS1 based tests are sensitive and specific as DIVA test (Birch-Machin et al 1997;Golchinfar et al 2014;Ozaki et al 2001;Takeyama et al 2011), while others have found the reverse (Avellaneda et al 2010). In this study, the accuracy of NS1-based ELISA as DIVA test was evaluated thoroughly by analyzing different fragments of the protein and different condition of ELISA.…”

Section: Introductionmentioning

confidence: 91%

See 1 more Smart Citation

The reliability of DIVA test based on M2e peptide exceed those based on HA2 or NS1 peptides

Tarigan¹,

Sumarningsih²,

Ignjatovic

2015

JITV

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Salah satu kelemahan utama vaksinasi pada avian influenza adalah bahwa vaksinasi tersebut tidak dapat memberikan perlindungan terhadap infeksi. Bila peternakan yang menerapkan vaksinasi terpapar virus dalam jumlah besar, infeksi subklinis dapat menyebar dalam peternakan tersebut dalam waktu yang lama tanpa diketahui. Kondisi tersebut hanya dapat dimonitor dengan alat tes DIVA (differentiation of infected from vaccinated animals), alat uji konvensional tidak dapat digunakan. Tes DIVA berdasarkan antibodi yang terbentuk akibat stimulasi virus bereplikasi merupakan tes DIVA yang paling sesuai. Untuk influenza H5N1 antibodi yang dimaksud antara lain antibodi terhadap M2e, protein NS1 dan peptida HA2 (HA_488-516). Tujuan penelitian ini adalah membandingkan level antibodi terhadap peptida M2e, NS1 dan HA2 pada ayam normal, vaksinasi dan infeksi (1, 2-3, ≥4 minggu pasca infeksi). Level antibodi diukur dengan ELISA menggunakan sintetik peptida sebagai antigen koting. Peptida yang digunakan antara lain: 4 buah peptida NS1 yang didasarkan pada berbagai lokasi pada protein NS1, peptida M2e dan HA2. Semua peptida dibiotinilasi pada ujung N nya. Koting peptida pada microtitre plate dilakukan secara langsung atau melalui jembatan streptavidin. Penelitian ini menunjukkan bahwa vaksinasi tidak merangsang pembentukan antibodi terhadap semua peptida. Ayam yang terinfeksi membentuk antibodi dengan level yang tinggi terhadap peptida M2e, tetapi sangat rendah terhadap peptida NS1 dan HA2. Antibodi terhadap peptida NS1 dan HA2 hanya dapat dideteksi dengan ELISA streptavidin-peptida. ELISA berbasis NS1 atau HA2 tidak dapat diandalkan sebagai tes DIVA untuk penyakit AI H5N1 pada ayam.

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Section: Discussionsupporting

confidence: 51%

Section: Introductionmentioning

confidence: 91%

The reliability of DIVA test based on M2e peptide exceed those based on HA2 or NS1 peptides

Tarigan¹,

Sumarningsih²,

Ignjatovic

2015

JITV

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“…Currently, it is widely accepted that NS1 is a non-structural protein that is only expressed in cells after infection and is not part of the virion. Anti-NS1 antibodies have been found in the sera of birds experimentally infected with different AIV subtypes, suggesting that NS1 antibodies could be used for diagnostic purposes [28][29][30] . However, no studies, so far, have reported the detection of NS1 antigen specifically in the brain of turkeys.…”

Section: Discussionmentioning

confidence: 99%

In turkeys, unlike chickens, the non-structural NS1 protein does not play a significant role in the replication and tissue tropism of the H7N1 avian influenza virus

Kuryshko,

Landmann,

Luttermann

et al. 2024

Preprint

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The economic losses caused by high pathogenicity (HP) avian influenza viruses (AIV) in poultry industry worldwide are enormous. Although chickens and turkeys are two closely related Galliformes, turkeys are thought to be a bridging host for the adaptation of AIV from wild birds to poultry because of their high susceptibility to AIV infections. HPAIV evolve from low pathogenicity (LP) AIV after circulation in poultry through mutations in different viral proteins, including the non-structural protein (NS1), a major interferon (IFN) antagonist of AIV. At present, it is largely unknown whether the virulence determinants of HPAIV are the same in turkeys and chickens. Previously, we showed that mutations in the NS1 of HPAIV H7N1 significantly reduced viral replication in chickens in vitro and in vivo. Here, we investigated the effect of NS1 on the replication and virulence of HPAIV H7N1 in turkeys after inoculation with recombinant H7N1 carrying a naturally truncated wild-type NS1 (with 224 amino-acid "aa" in length) or an extended NS1 with 230-aa similar to the LP H7N1 ancestor. There were no significant differences in multiple-cycle viral replication or in the efficiency of NS1 to block IFN induction in cell culture. Similarly, all viruses were highly virulent in turkeys and replicated at similar levels in various organs and swabs collected from inoculated turkeys. These results suggest that NS1 does not play a role in the virulence or replication of HPAIV H7N1 in turkeys and further indicate that the genetic determinants of HPAIV differ in these two closely related galliform species.

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“…To investigate whether the influenza A(H5N2) antibodies were elevated as a result of exposure to that virus or because of vaccination with heterologous influenza viruses, we determined antibody levels to influenza A(H5N2) nonstructural protein 1 (NS1) ( 12 – 15 ). The NS1 protein is not readily incorporated into virions used to make inactivated influenza vaccine, so a response to NS1 protein would indicate active influenza A(H5N2) infection.…”

Section: The Studymentioning

confidence: 99%

Influenza A(H5N2) Virus Antibodies in Humans after Contact with Infected Poultry, Taiwan, 2012

Wu¹,

Yang²,

Liu³

et al. 2014

Emerg. Infect. Dis.

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Detection of highly pathogenic avian influenza virus infection in vaccinated chicken flocks by monitoring antibodies against non-structural protein 1 (NS1)

Cited by 7 publications

References 32 publications

The reliability of DIVA test based on M2e peptide exceed those based on HA2 or NS1 peptides

The reliability of DIVA test based on M2e peptide exceed those based on HA2 or NS1 peptides

In turkeys, unlike chickens, the non-structural NS1 protein does not play a significant role in the replication and tissue tropism of the H7N1 avian influenza virus

Influenza A(H5N2) Virus Antibodies in Humans after Contact with Infected Poultry, Taiwan, 2012

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