Detection of histidine decarboxylase in rat aorta and cultured rat aortic smooth muscle cells

Abstract

The results of this study are the first to demonstrate the presence of HDC mRNA and protein in rat aorta and more specifically in RASMC, indicative of their capability to synthesize histamine.

Effects of IL-3 and SCF on Histamine Production Kinetics and Cell Phenotype in Rat Bone Marrow-derived Mast Cells

“…This amine imparts its biological activities by binding histamine receptors on the cell membrane of non-classical histamine binding sites, in addition to its itching effect observed in allergic reactions (17,32). As an indicator of mast cell maturation, histamine levels were analyzed (Fig.…”

“…cDNA was synthesized by incubating RNA at 25℃ for 10 min, at 48℃ for 30 min, and then at 95℃ for 5 min with the SUPERSCRIPT reverse transcriptase (Invitrogen, Carlsbad, CA, USA) in a PTC-100 thermal cycler (MJ Research, Inc. Watertown MA, USA). HDC real time PCR primers included the following: sense, 5'-GCA GCA AGG AAG AAC AAA ATC C-3'; antisense, 5'-CAA CAA GAC GAG CGT TCA GAG A-3'; probe, FAM-AAA GCG CAT GAG CCC AAT GCT GAT-TAMRA (31,32). These were purchased from Bioneer (Daedockgu, Daejeon South Korea).…”

Up-regulation of histidine decarboxylase expression and histamine content in B16F10 murine melanoma cells

“…coli transfected with rat recombinant HDC was kindly provided by Dr. Lo Persson (University of Lundt, Sweden) and used as described previously [11]. HDC cDNA had been inserted into a plasmid containing an ampicillin resistance gene.…”

Biosensor cell assay for measuring real-time aldosterone-induced release of histamine from mesenteric arteries

“…HDC has previously been shown to be expressed in vascular smooth muscle cells (VSMCs) from rats and in human endothelial and VSMCs indicating that these cells types have the ability to synthesis histamine (Tippens et al . , Tippens & Gruetter ).…”