Detection of Human Cytomegalovirus UL97 D605E Mutation in Korean Stem Cell Transplantation Recipients and Donors

Lee, Gyu-Cheol; Choi, Su‐Mi; Lee, Chan Hee; Lee, Dong Gun; Choi, Jun-Sub; Yoo, Jin‐Hong

doi:10.4014/jmb.1303.03094

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…Zhou be considered as a natural sequence variant. The prevalence of the D605E mutation was 91.8 %, 100 %, and 89.2 %, respectively, in recipients from Japan, Korea, and Taiwan and is therefore considered an important molecular marker of HCMV strains circulating in East Asian countries (2,(22)(23)(24). Some authors suggested that the D605E mutation could partially or fully compensate for the effects of the GCV resistance, conferred by M460V or A594P (25).…”

Section: Discussionmentioning

confidence: 99%

Cytomegalovirus UL97 ganciclovir resistance mutations in kidney transplant recipients

Tasoujlu¹,

Khalafkhany²,

Makhdoomi³

et al. 2022

BLL

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OBJECTIVES: Widespread and prolonged therapy with ganciclovir (GCV) may result in the emergence of GCV-resistant mutations in human cytomegalovirus (HCMV) genome. The aim of this study was to detect the UL97 mutations associated with GCV resistance in kidney transplant recipients. METHODS: Forty-nine kidney recipients with positive HCMV DNAemia, who received GCV therapy were included in this study. A 707 bp fragment of UL97 gene spanning codons (436 to 655) was amplifi ed by nested PCR and sequenced. RESULTS: Thirteen (26.5 %) out of 49 recipients contained mutations associated with amino acid changes. Two UL97 mutations related to GCV resistance were detected in 2 recipients (4 %), including alanine to valine (A594V) and proline to leucine (P521L). The D605E mutation was identifi ed in 8 out of 49 (16.3 %) recipients. Silent mutations G598G, G503G, L553L, L634L, D456D and G579G were commonly observed. CONCLUSION: Our results indicate that mutations in the UL97 gene associated with GCV resistance may occur in 1 in 25 recipients treated with GCV. In addition, a higher mutation rate of D605E was detected in our recipients. This study provides the fi rst evidence of the prevalence and pattern of GCV related mutations in Iranian Turkish recipients (Tab. 2, Fig. 1, Ref. 28).

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Section: Discussionmentioning

confidence: 99%

Cytomegalovirus UL97 ganciclovir resistance mutations in kidney transplant recipients

Tasoujlu¹,

Khalafkhany²,

Makhdoomi³

et al. 2022

BLL

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“…In patients with HIV and AIDS, due to immune deficiency, the antibody against CMV is low or no detectable, therefore, it is necessary to use molecular methods such as PCR to detect and identify CMV-infected patients. Also, Due to several mutations in the main genes of the CMV virus, progression patients with HIV, can be facilitates to diseases such as retinitis, encephalitis and ultimately death [31][32][33].…”

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confidence: 99%

Evaluation Frequency of Human Cytomegalovirus Resistance to Ganciclovir (UL97) in Patients with HIV, using High Resolution Melting Analysis, Kerman, Iran

Arefinia

Mollaei

Arabzadeh

et al. 2018

BJSTR

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Background: To evaluate frequency of Cytomegalo virus resistant to ganciclovir a cross-sectional study was designed.Methods: In this study 96 samples from patients infected with HIV, 41 males and 55 Females with Mean ± SD years old age 52.6 ± 6.9 and 42.6 ± 9.1 respectively were collected. The both buffy coat and plasma were tested using Real Time PCR for detection of CMV, because the CMV virus latent in Peripheral Blood Mono Nuclear cells (PBMNs).Results: Out of 96 patients, twenty-two (22.9%) buffy coat samples, and seven plasma samples (7.3%) were positive for CMV. A High-Resolution Melting (HRM) curve assay was performed for detection of CMV resistant to ganciclovir in UL97 region. Out of 22 CMV positive samples from buffy coat, there were five samples resistance to ganciclovir (22.72%), three M460V, one M460I and one mix genotype of M460V/I. Out of seven positive samples from plasma four samples (57.14%) positive for resistance to ganciclovir. Conclusion:Mutation in UL97 and UL54 regions can be resistance of CMV to ganciclovir. High-level resistance to ganciclovir often is associated with cross resistance to other polymerase inhibitors such as cidofovir. Most point mutations lead to resistance to treatment with ganciclovir was done at 460,594 and 595 amino acid location in UL97 gene. Today, we are witnessing the emergence of drug resistance in viruses. This causes diseases that previous therapies do not affect and cause mortality in humans. Therefore, the evaluation of drug resistance in viruses is of importance and can provide a better approach to physicians in treating their patients.

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Human Cytomegalovirus PUL97 Drug-Resistance Mutations in Congenitally Neonates and HIV-infected, No-Drug-Treated Patients

et al. 2016

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Aim: Human cytomegalovirus (HCMV) treatment is hard to achieve because of viral protein target sequence variations. Objectives: We aimed to find HCMV pUL97 kinase variations in HIV- and congenitally infected patients. Methods: Twenty HCMV-positive DNA samples from nonganciclovir treated congenitally infected neonates and HIV positive patients were used for PCR restriction fragment length polymorphism. Variations were assessed computationally for pUL97 functionality. Results: P521L, D605E and N597Y substitutions were prevalent significantly in congenital infection. Furthermore, we found those mutations have neutral or low impact on pUL97 functionality. In addition, we found a new K599Q substitution in an HIV-infected individual. Conclusion: More prevalent substitutions related to low-grade ganciclovir resistance were found in congenitally infected neonates in comparison with HIV-infected patients.

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Detection of Human Cytomegalovirus UL97 D605E Mutation in Korean Stem Cell Transplantation Recipients and Donors

Cited by 3 publications

References 23 publications

Cytomegalovirus UL97 ganciclovir resistance mutations in kidney transplant recipients

Cytomegalovirus UL97 ganciclovir resistance mutations in kidney transplant recipients

Evaluation Frequency of Human Cytomegalovirus Resistance to Ganciclovir (UL97) in Patients with HIV, using High Resolution Melting Analysis, Kerman, Iran

Human Cytomegalovirus PUL97 Drug-Resistance Mutations in Congenitally Neonates and HIV-infected, No-Drug-Treated Patients

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