Detection of Human IgG on Poly(pyrrole-3-carboxylic acid) Thin Film by Electrochemical-Surface Plasmon Resonance Spectroscopy

ACS Appl. Mater. Interfaces

Baba

Phanichphant³

et al. 2012

Self Cite

In this study, we describe the combination of transmission surface plasmon resonance (TSPR) and electrochemical techniques for the application to biosensors with conducting polymers. Electropolymerization was employed to construct poly(pyrrole-3-carboxylic acid) (PP3C) film on a gold-coated grating substrate using pyrrole-3-carboxylic acid (P3C) monomer solution in 0.5 M H(2)SO(4). In situ electrochemical-transmission surface plasmon resonance (EC-TSPR) measurements were carried out to study the kinetic and electroactivity properties of PP3C film. Immobilization of antihuman IgG on the activated surface and the binding process of human IgG and antihuman IgG in neutral solution could be detected in situ by EC-TSPR measurement. The surface modification steps on the PP3C layer led to an increase in intensity of the transmission peak. The performance, sensitivity, and utility of EC-TSPR spectroscopy showed obvious advantages for the detection of binding process with the simple experimental setup, and could be applied to the study of biomolecular interactions in various systems.

Section: Introductionmentioning

confidence: 99%

In situ Electrochemical-Transmission Surface Plasmon Resonance Spectroscopy for Poly(pyrrole-3-carboxylic acid) Thin-Film-Based Biosensor Applications

ACS Appl. Mater. Interfaces

Baba

Phanichphant³

et al. 2012

Self Cite

“…and k off could be obtained by fitting the dissociation curves with eq. , which express below:

R = R_{eq} true{1 - exp true[- (k_{on} c - k_{off}) t true] true}

R = R_{eq} exp true(- k_{off} t true)

where R is reflectivity response and R eq is the equilibrium reflectivity response at the target concentration of c . The calculated k on and k off are shown in Table .…”

Section: Resultsmentioning

confidence: 99%

Development of an electrochemical‐surface plasmon dual biosensor based on carboxylated conducting polymer thin films

Sriwichai

J of Applied Polymer Sci

Phanichphant

et al. 2017

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A biosensing platform based on the covalent attachment of biomolecules on electropolymerized carboxylated conducting polymers, poly(3-aminobenzoic acid) and poly(3-pyrrole carboxylic acid), were developed for the selective simultaneous detection of two biomolecules using electrochemical-surface plasmon resonance (EC-SPR) spectroscopy. The surface morphology of the developed biosensors was studied by scanning electron microscopy and atomic force microscopy. The EC-SPR dual biosensor was developed for the label-free, simultaneous, and selective detection of glucose and human immunoglobulin G (IgG). A change in current density was clearly observed after the injection of glucose, whereas a change in SPR reflectivity was clearly observed after the injection of human IgG. The present work demonstrates the potential of this biosensing platform for real sample analysis in the future. V C 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 45641.

“…Polypyrrole and its derivative were studied for the biosensor application [26,34]. The EC-SPR spectroscopy can be applied not only in monitoring the electropolymerization of polymer films, but also in illustrating the properties of immunosensors [12,38,39]. In 2004, the label-free immunosensor for the detection of a rabbit IgG antigen by an antirabbit IgG antibody at the surface of polypyrrole (PPy) membrane was reported by Gooding et al [13].…”

Section: Spr Biosensors Based On Functional Conducting Polymersmentioning

confidence: 99%

“…After rinsing with PBS, constant potential was applied for 1 min before adding human IgG for sensing experiment. The SPR reflectivity Recently, poly(pyrrole-3-carboxylic acid)-(PP3C-) based immunosensor investigated by in situ EC-SPR for monitoring the immobilization of anti-human IgG and its interaction with human IgG was reported by Janmanee et al [39]. A pyrrole-3-carboxylic acid monomer was used for electropolymerization of PP3C film on gold surface.…”

Section: Journal Of Nanotechnologymentioning

confidence: 99%

Functional Conducting Polymers in the Application of SPR Biosensors

Journal of Nanotechnology

Chuekachang

Sriwichai

et al. 2012

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In recent years, conducting polymers have emerged as one of the most promising transducers for both chemical, sensors and biosensors owing to their unique electrical, electrochemical and optical properties that can be used to convert chemical information or biointeractions into electrical or optical signals, which can easily be detected by modern techniques. Different approaches to the application of conducting polymers in chemo-or biosensing applications have been extensively studied. In order to enhance the application of conducting polymers into the area of biosensors, one approach is to introduce functional groups, including carboxylic acid, amine, sulfonate, or thiol groups, into the conducting polymer chain and to form a so-called "self-doped" or by doping with negatively charged polyelectrolytes. The functional conducting polymers have been successfully utilized to immobilize enzymes for construction of biosensors. Recently, the combination of SPR and electrochemical, known as electrochemical-surface plasmon resonance (EC-SPR), spectroscopy, has been used for in situ investigation of optical and electrical properties of conducting polymer films. Moreover, EC-SPR spectroscopy has been applied for monitoring the interaction between biomolecules and electropolymerized conjugated polymer films in biosensor and immunosensor applications. In this paper, recent development and applications on EC-SPR in biosensors will be reviewed.