Development of an electrochemical‐surface plasmon dual biosensor based on carboxylated conducting polymer thin films

Abstract

A biosensing platform based on the covalent attachment of biomolecules on electropolymerized carboxylated conducting polymers, poly(3-aminobenzoic acid) and poly(3-pyrrole carboxylic acid), were developed for the selective simultaneous detection of two biomolecules using electrochemical-surface plasmon resonance (EC-SPR) spectroscopy. The surface morphology of the developed biosensors was studied by scanning electron microscopy and atomic force microscopy. The EC-SPR dual biosensor was developed for the label-free, simultaneous, and selective detection of glucose and human immunoglobulin G (IgG). A change in current density was clearly observed after the injection of glucose, whereas a change in SPR reflectivity was clearly observed after the injection of human IgG. The present work demonstrates the potential of this biosensing platform for real sample analysis in the future. V C 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 45641.

Polyaniline Coated Core-Shell Typed Stimuli-Responsive Microspheres and Their Electrorheology

“…Recently, conducting polymer materials have been studied widely in many fields because of their low density, good thermal and chemical stability, reproducibility, and controllable conductivity [ 11 , 12 , 13 ]. Polyaniline (PANI), which is one of the most promising and extensively investigated conducting polymers, has been applied extensively to several areas, such as supercapacitors [ 14 ], catalysts [ 15 ], sensors [ 16 ], and biological fields [ 17 ], because of its electro-activity, environmental and chemical stability, controllable conductivity, biological compatibility, ease of preparation, and low cost [ 18 ].…”

Perspective potential of polymer-based biosensor chips in food industry and clinical diagnostics

“…The polyurethane layer that can be part of glucose biosensor is responsible for biosensor sensitivity and intensity of detection signal [11,12]. Poly(lactic acid) (PLA) [3,[15][16][17][18] Poly(lactic-co-glycolic acid) (PLGA) [4,5,18,24,[27][28][29][30][31] Poly(3,4-ethylenedioxythiopene) [24][25][26] Poly(3-aminobenzoic acid) [24,[27][28][29] Poly(pyrrole-3--carboxylic acid) [24,30,31] The polymers which are distinguished by their biocompatibility and biodegradability are especially applicable in medical purposes. Here we are talking about synthetic polymers, such as poly(lactic acid) (PLA) and its copolymer (e.g., poly(lactic-co-glycolic acid) (PLGA)), which are approved by Food and Drug Administration (FDA) and commonly used in drug targeting and biosensor production.…”

“…It has been used in construction of conducting polymer film, as a part of the biosensor´s build [27,28]. In combination with other polymers, such as polyaniline, it can be applied for construction of immunosensors in which mechanism of action is based on the surface plasmon resonance (SPR) [29].…”

Electrochemical Dopamine Biosensor Based on Poly(3-aminobenzylamine) Layer-by-Layer Self-Assembled Multilayer Thin Film

“…Conducting polymers have received great attention for electrochemical applications because the π-bond delocalized electrons on the conjugated structures lead to excellent electrical and conductive properties [ 18 , 19 ]. The common conducting polymers for electrochemical biosensors are polyaniline (PANi) [ 20 ], polypyrrole (PPy) [ 21 ], poly(3,4-ethylenedioxythiophene) (PEDOT) [ 22 ] and polythiophene (PTH) [ 23 ].…”