Detection of Human Papillomavirus DNA in Colorectal Adenomas

Cheng, Jhy-Young; Sheu, Lai-Fa; Lin, Jerry W.; Meng, Ching-Liang

doi:10.1001/archsurg.1995.01430010075015

Cited by 22 publications

(15 citation statements)

References 27 publications

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“…Data on the relationship between HPV infection and other factors, including genetic and molecular changes, and the development of CRC from adenomas are not conclusive. Cheng et al (1995) found HPV DNA in 28% of 109 paraffin-embedded adenomas. In contrast, Burnett-Hartman et al (2013) did not find HPV DNA in an assessment of 167 colorectal adenomas, 87 hyperplasic polyps, and 250 polyp-free controls.…”

Section: Discussionmentioning

confidence: 99%

Molecular evidence of high-risk human papillomavirus infection in colorectal tumours from Cuban patients

Soto

Limia

González

et al. 2016

Mem. Inst. Oswaldo Cruz

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The association between colorectal cancer and human papillomavirus (HPV) infection is still unproven. The aim of this study was to investigate the presence of high-risk HPV (HR-HPV) DNA in colorectal tissues from Cuban patients. A total of 63 colorectal formalin-fixed paraffin-embedded tissues were studied (24 adenocarcinoma, 18 adenoma, and 21 colorectal tissues classified as benign colitis). DNA from colorectal samples was analysed by quantitative real-time polymerase chain reaction to detect the most clinically relevant high HR-HPV types (HPV-16, -18, -31, -33, -45, -52, and -58). Associations between histologic findings and other risk factors were also analysed. Overall, HPV DNA was detected in 23.8% (15/63) of the samples studied. Viral infections were detected in 41.7% of adenocarcinoma (10/24) and 27.7% of adenoma cases (5/18). HPV DNA was not found in any of the negative cases. An association between histological diagnosis of adenocarcinoma and HPV infection was observed (odd ratio = 4.85, 95% confidence interval = 1.40-16.80, p = 0.009). The only genotypes identified were HPV 16 and 33. Viral loads were higher in adenocarcinoma, and these cases were associated with HPV 16. This study provides molecular evidence of HR-HPV infection in colorectal adenocarcinoma tissues from Cuban patients.

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Section: Discussionmentioning

confidence: 99%

Molecular evidence of high-risk human papillomavirus infection in colorectal tumours from Cuban patients

Soto

Limia

González

et al. 2016

Mem. Inst. Oswaldo Cruz

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“…In previous studies of colorectal adenomas, HPV DNA detection varied, ranging from 0–38% (11, 12, 22), and one of these studies reported a statistically significant difference in the proportion of adenomas positive for HPV DNA (38%) compared to normal colonic tissue from disease-free controls (8%) (12). However, PCR, and particularly nested PCR, is prone to contamination.…”

Section: Discussionmentioning

confidence: 99%

No Evidence for Human Papillomavirus in the Etiology of Colorectal Polyps

Burnett‐Hartman

Newcomb

Mandelson

et al. 2011

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Background While some studies have reported detection of oncogenic human papillomavirus (HPV) in colorectal tumors, others have not. Methods We examined the association between oncogenic HPV infection and colorectal polyps in a case-control study of individuals with colorectal adenomas (n=167), hyperplastic polyps (n=87), and polyp-free controls (n=250). We performed real-time PCR for HPV-16 /18 DNA, and SPF PCR covering 43 HPV types, on lesional and normal colorectal tissue samples. Plasma antibodies for oncogenic HPV types were assessed via a bead-based multiplex Luminex assay. Results HPV DNA was not found in any of the 609 successfully assayed colorectal tissue samples from adenomas, hyperplastic polyps, normal biopsies adjacent to polyps, or normal biopsies of the rectum of disease-free controls. Also, there was no association between HPV seropositivity for all oncogenic HPV types combined, for either polyp type, and for men or women. When analyses were restricted to participants without a previous history of polyps, among men [adenomas (n=31), hyperplastic polyps (n=28), and controls (n=68)], there was an association between seropositivity and hyperplastic polyps when all oncogenic HPV types were combined (odds ratio=3.0; 95% confidence interval: 1.1–7.9). Conclusions Overall, our findings do not support an etiologic relationship between HPV and colorectal adenomas or hyperplastic polyps; however, our finding suggesting an association between HPV seropositivity and hyperplastic polyps in men may warrant further investigations. Impact After stringent controls for contamination and three methods to assess HPV infection, we report no evidence for HPV in the etiology of colorectal neoplasia for either men or women.

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“…Despite the variation in the control specimen, all studies confirmed an association between HPV detection rates, specifically subtypes 16 and 18, and CRC with odd ratio ranging between 2.7 (95% CI, 1.1-6.2) and 9.1 (95% CI, 3.7-22.3). (Cheng et al, 1995;Kirgan et al, 1990). Moreover, the strength of association was related to the degree of tumour dysplasia.…”

Section: Cancer In Humanmentioning

confidence: 95%