Detection of hydrogen peroxide produced by microorganisms on an ABTS peroxidase medium

Müller, H. E.

doi:10.1016/s0176-6724(85)80045-6

Cited by 86 publications

(64 citation statements)

References 11 publications

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“…Hydrogen peroxide scavenging activity was determined according to the method of Müller (1985). Briefly, 100 µL of 0.1 M phosphate buffer (pH 5.0) was mixed with each extract in a 96-well plate.…”

Section: Preparation Of Velvet Antler Extractsmentioning

confidence: 99%

Antioxidant, Anti-acetylcholinesterase and Composition of Biochemical Components of Russian Deer Velvet Antler Extracts

Je¹,

Park²,

Lim³

et al. 2011

Korean Journal for Food Science of Animal Resources

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Russian deer velvet antlers were divided into three parts and subjected to a extraction process using hot water at 100, 110, and 120 o C or an extraction with 70% ethanol. Each extract was analyzed for its biochemical components, including uronic acid, sulfated-glycosaminoglycans (sulfated-GAGs), and sialic acid, and the antioxidant and anti-acetylcholinesterase activities were investigated. Different levels of uronic acid and sulfated-GAGs were observed in the extracts according to the water temperature used for the extraction, and contents decreased with increasing extraction temperature. The upper layer of each extract showed high amounts of uronic acid and sulfated-GAGs, followed by the middle and base layers. Ethanol extraction was more effective for recovering uronic acid than sulfated-GAGs. Sialic acid content was the highest in the 110 o C extracts but was not observed in the ethanol extracts. Velvet antler extracts showed strong antioxidant activities against DPPH and hydrogen peroxide as well as strong reducing power in a dose-dependent manner. However, the antioxidant activities were different in each layer and according to the extraction method. Additionally, velvet antler extracts exhibited inhibitory activity against acetylcholinesterase, which is associated with Alzheimer's disease, in a dose-dependent manner. These results suggest that velvet antler extracts are useful as a functional food ingredient and/or a pharmaceutical.

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Section: Preparation Of Velvet Antler Extractsmentioning

confidence: 99%

Antioxidant, Anti-acetylcholinesterase and Composition of Biochemical Components of Russian Deer Velvet Antler Extracts

Je¹,

Park²,

Lim³

et al. 2011

Korean Journal for Food Science of Animal Resources

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“…ABTS RSA The ABTS RSA was evaluated using the method described by (Muller, 1985). Each extract (0.1 mL) was mixed with potassium phosphate buffer (0.1 mL, 0.1 M, pH 5.0) and hydrogen peroxide (20 μL, 10 mM), and preincubated at 37℃ for 5 min.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity and Fatty Acid Composition of Four Different Persimmon Seeds

Jang

Bae

et al. 2010

FSTR

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This study was conducted to determine antioxidant activities in acetone, ethanol, methanol, and water extracts from the seeds of 4 persimmon cultivars. Antioxidant activities were evaluated on the basis of DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging activity (RSA), ABTS (2,2-azino-bis [3-ethylbenzothiazoline-6-sulfonic acid]) RSA, and reducing power. Cheongdobansi seed extracts showed significantly higher antioxidant activities and phenolic contents than did Bongok, Fuyu, and Nishimurawase seed extracts. Ethanol was more effective for the extraction of antioxidant compounds from persimmons than other solvents. Palmitic acid, oleic acid, and linoleic acid were the main fatty acids found in persimmon seeds. Fuyu seeds contained the highest levels of fatty acids, and the unsaturated fatty acid contents of persimmon seeds range from 70.4% to 78.3%. All persimmon seed extracts, with the exception of the water extract of Nishimurawase seeds, inhibited DNA damage induced by H 2 O 2 .

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“…Hydrogen peroxide (H 2 O 2 ) Scavenging Activity: H 2 O 2 scavenging activity was determined according to the method of Muller 18 . DMSO with sample was used as a blank.…”

Section: Reducing Power Assaymentioning

confidence: 99%

Untitled

2017

IJPSR

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concentration. In nitric oxide radical scavenging activity, the ascidians fractions E. viride II showed 64% at 100µgmL -1 and D. psammathodes II showed 72% at 100µgmL -1 concentration. The finding throws light on the higher efficiency of scavenging activity of natural products improved to synthetic drugs. These results indicate that ascidian represent a promising biological resource for derivation of new compounds with antioxidant potential which was far reaching implication in biomedical research and therapeutics.

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Detection of hydrogen peroxide produced by microorganisms on an ABTS peroxidase medium

Cited by 86 publications

References 11 publications

Antioxidant, Anti-acetylcholinesterase and Composition of Biochemical Components of Russian Deer Velvet Antler Extracts

Antioxidant, Anti-acetylcholinesterase and Composition of Biochemical Components of Russian Deer Velvet Antler Extracts

Antioxidant Activity and Fatty Acid Composition of Four Different Persimmon Seeds

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