Detection of hydroxyl radicals produced by wood-decomposing fungi

Tornberg, Karin

doi:10.1016/s0168-6496(02)00200-3

Cited by 11 publications

(19 citation statements)

References 13 publications

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“…On the contrary, both fungi showed a slight stimulatory effect on the numbers of bacteria in soil adhering to exploratory hyphae. The stimulation of bacterial numbers in soil partly supports the findings of a previous study (Tornberg & Olsson, 2002) in which PLFA analysis revealed a slight increase of bacterial biomass in soil colonized by R. bicolor , but not by H. fasciculare . In another study, opportunistic soil bacteria were stimulated by H. fasciculare (Gramss et al , 1999), perhaps as a result of growth on mycelial exudates.…”

Section: Discussionsupporting

confidence: 89%

Impact of white-rot fungi on numbers and community composition of bacteria colonizing beech wood from forest soil

Folman

Gunnewiek

Boddy

et al. 2008

FEMS Microbiology Ecology

134

106

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White-rot fungi are important wood-decomposing organisms in forest ecosystems. Their ability to colonize and decompose woody resources may be strongly influenced by wood-inhabiting bacteria that grow on easily utilizable compounds e.g. oligomers of wood-polymers released by fungal enzymes. However, so far, it is not known how white-rot fungi deal with the presence of potential competing bacteria. Here, the effects of two white-rot fungi, Hypholoma fasciculare and Resinicium bicolor, on the numbers and composition of bacteria colonizing sterile beech wood blocks from forest soil are reported. Both total numbers (microscopic counts) and the numbers of cultivable wood-inhabiting bacteria were considerably lower in wood blocks that became colonized by the white-rot fungi than in control blocks. This points to the fungi out-competing the opportunistic bacteria. The presence of white-rot fungi resulted in a change in the relative abundance of families of cultivable bacteria in wood and also in a change of denaturing gradient gel electrophoresis patterns of directly amplified 16S rRNA gene fragments. Analysis of the bacterial community structure in soil adhering to exploratory mycelium (cords) indicated that fungal species-specific effects on bacterial community composition were also apparent in this fungal growth phase.

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Section: Discussionsupporting

confidence: 89%

Impact of white-rot fungi on numbers and community composition of bacteria colonizing beech wood from forest soil

Folman

Gunnewiek

Boddy

et al. 2008

FEMS Microbiology Ecology

134

106

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“…One simple solution would be to use higher initial 3‐CCA concentration in the medium. However, tests of fungal tolerance of 3‐CCA indicate a fungistatic effect of elevated 3‐CCA concentrations [8] that is in agreement with the known antimicrobial activity of coumarins [20,21]. Alternatively, the 7‐OHCCA fluorescence can be measured after a short incubation time, before a large proportion of the 3‐CCA has been transformed to HBMA.…”

Section: Discussionsupporting

confidence: 66%

“…Consequently, wood‐decaying fungi that are able either to degrade lignin (white‐rot fungi) or modify it (brown‐rot fungi) may affect 3‐CCA and/or 7‐OHCCA, which may cause an underestimation of the • OH formation. Results of the study by Tornberg and Olsson [8] indicated that such underestimation can vary from 10 to 30% of the control value. Therefore, the risk of fungal modification of 3‐CCA and 7‐OHCCA needs to be assessed.…”

Section: Introductionmentioning

confidence: 94%

“…To be able to study the mechanism behind fungal wood‐degradation it is important to have a reliable method to estimate the production of • OH. In a previous study, Tornberg and Olsson [8] detected • OH produced by wood‐decomposing fungi. These authors used a method based on hydroxylation of coumarin‐3‐carboxylic acid (3‐CCA) by • OH, a reaction that produces one major fluorescent product, 7‐hydroxycoumarin‐3‐carboxylic acid (7‐OHCCA) [9–11].…”

Section: Introductionmentioning

confidence: 95%

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Fungal modification of the hydroxyl radical detector coumarin-3-carboxylic acid

Iakovlev

Broberg

Stenlid

2003

FEMS Microbiology Ecology

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The feasibility of using coumarin-3-carboxylic acid (3-CCA) for detection of hydroxyl radicals in pure cultures of wood-decaying fungi was tested. Fungi were incubated on a 3-CCA-containing medium. The transformation of 3-CCA to the fluorescent hydroxyl radical detector substance 7-hydroxycoumarin-3-carboxylic acid and other compounds was studied by chromatographic and spectroscopic techniques. During incubation of all tested fungi, a small fraction of the 3-CCA was hydroxylated to 7-hydroxycoumarin-3-carboxylic acid and a major fraction of the 3-CCA was metabolized by fungi to 2-(2-hydroxybenzyl)malonic acid. In most cultures the concentration of 3-CCA was below detection limit at the end of incubation. The fungal metabolism was suggested to be involved in the formation of 2-(2-hydroxybenzyl)malonic acid from 3-CCA, consequently making this method of hydroxyl radical detection less suitable to use on cultures of wood-decaying fungi.

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“…). Similarly, fungi produce reactive oxygen species which regulates their cellular differentiation and development and facilitates defence against antagonistic bacteria (Tornberg & Olsson ; Tudzynski et al . ).…”

Section: Discussionmentioning

confidence: 99%

Midgut fungal and bacterial microbiota of Aedes triseriatus and Aedes japonicus shift in response to La Crosse virus infection

et al. 2016

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Understanding how midgut microbial communities of field-collected mosquitoes interact with pathogens is critical for controlling vector infection and disease. We used 16S rRNA and internal transcribed spacer sequencing to characterize the midgut bacterial and fungal communities of adult females of Aedes triseriatus and Aedes japonicus collected as pupae in tree holes, plastic bins and waste tires and their response to La Crosse virus (LACV) infection. For both mosquito species and across all habitat and virus treatments, a total of 62 bacterial operational taxonomic units (OTUs) from six phyla and 21 fungal OTUs from two phyla were identified. The majority of bacterial (92%) and fungal (71%) OTUs were shared between the mosquito species; however, several OTUs were unique to each species. Bacterial and fungal communities of individuals that took either infectious or noninfectious bloodmeals were less diverse and more homogeneous compared to those of newly emerged adults. Interestingly, LACV-infected A. triseriatus and A. japonicus had higher bacterial richness and lower fungal richness compared to individuals that took a noninfectious bloodmeal, suggesting that viral infection was associated with an increase in bacterial OTUs and a decrease in fungal OTUs. For both mosquito species, several OTUs were identified that had both high fidelity and specificity to mosquito midguts that were infected with LACV. Overall, these findings demonstrate that bacterial and fungal communities that reside in mosquito midguts respond to host diet and viral infection and could play a role in modulating vector susceptibility to LACV.

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Detection of hydroxyl radicals produced by wood-decomposing fungi

Cited by 11 publications

References 13 publications

Impact of white-rot fungi on numbers and community composition of bacteria colonizing beech wood from forest soil

Impact of white-rot fungi on numbers and community composition of bacteria colonizing beech wood from forest soil

Fungal modification of the hydroxyl radical detector coumarin-3-carboxylic acid

Midgut fungal and bacterial microbiota of Aedes triseriatus and Aedes japonicus shift in response to La Crosse virus infection

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