Detection of <i>Helicobacter Pylori</i> in Endoscopic Biopsies in Sudan

Mirghani, Yousra Abdelazim; Ahmed, Salah; Ahmed, Mohamed A.; Ismail, Maha; Fedail, S S; Kamel, Moemena; Saidia, Haruo

doi:10.1177/004947559402400407

Cited by 17 publications

(10 citation statements)

References 8 publications

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“…The prevalence in the remaining two studies, from Uganda and Sudan, was 25 and 39%, respectively. 18,19 Despite this, this review confirms the high prevalence of the organism in the gastric mucosa in most dyspeptic populations in Africa.…”

Section: Resultssupporting

confidence: 56%

Helicobacter pylori in Africa: Observations on an ‘enigma within an enigma’

Kidd

Louw

Marks

1999

J of Gastro and Hepatol

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Section: Resultssupporting

confidence: 56%

Helicobacter pylori in Africa: Observations on an ‘enigma within an enigma’

Kidd

Louw

Marks

1999

J of Gastro and Hepatol

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“…(59) However, molecular tests and PCR technology have been raised as a useful alternative means of bacterial identification, which may circumvent some of these difficulties (59,60) but has limited mainly by contamination and inadequate sensitivity issues. (61) (18,19,(62)(63)(64)(65) Tetracycline is one of the 30S-targeting antibiotics that inhibit translation elongation by sterically interfering with the binding of aminoacyl-tRNA to the A-site of the ribosome. (66) Therefore, mutations located in two domains (III and IV) in 16S rRNA: helix 34 and the loop next to helix 31 (67) can affect the conformation of the tetracycline-binding site, leading to high-level resistance.…”

Section: Discussionmentioning

confidence: 99%

Molecular Phylogenetic Analysis of16S rRNASequences Identified Two lineages ofH. pyloriStrains Detected from Different Regions in Sudan Suggestive of Differential Evolution

Idris

Hassan

Ali

et al. 2019

Preprint

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Background: H. pylori is ubiquitous among humans, and one of the best studied examples of an intimate association between bacteria and humans. Under several diverse socio-demographic factors in Sudan, a continuous increase in the prevalence rate of H. pylori infection has been noticed which represents a major public health challenge. In this study, we analyzed the molecular evolution of H. pylori Strains detected from different ethnic and regions of Sudan using 16S rRNA gene and phylogenetic approach. Materials and methods:A total of 75 gastric biopsies taken from patients who had been referred for endoscopy from different regions of Sudan. The DNA extraction was done by using the guanidine chloride method. Two sets of primers (universal and specific for H. pylori) were used to amplify the 16S ribosomal gene. Sanger sequencing was performed; then Blast these sequences with those available in the NCBI nucleotide database. The evolutionary aspects were analyzed using a MEGA7 software.Result: Molecular detection of H. pylori has shown that 28 (37.33%) of patients were positive for H. pylori. Bivariate analysis has found no significant differences exhibited across sociodemographic, endoscopy series and H. pylori infection. Nucleotide variations were found at five nucleotide positions (positions 219, 305, 578, 741 and 763-764) and one insertion mutation (750_InsC_751) was present in sixty-seven percent (7/12) of our strains. The phylogenetic tree diverged into two lineages. Conclusion:The phylogenetic analysis of 16S rRNA sequences identified two lineages of H. pylori strains detected from different regions in Sudan. Sex mutations were detected in regions of the 16S rRNA not closely associated with either tetracycline or tRNA binding sites. 66.67% of them were located in the central domain of 16S rRNA. Studying the effect of these mutations on the functions of 16S rRNA molecules in protein synthesis and antibiotic resistance is of great importance.

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“…These findings suggest that the lower population density and relative socio-economic isolation of the area are factors to consider in the low prevalence of H. pylori infection in this area of Uganda. Information regarding H. pylori infection in areas bordering Northern Uganda is scarce; a study in Sudan using the rapid urease test and culture to diagnose H. pylori infection found a prevalence of 16% in normal control subjects compared to 80% in patients with gastritis and 56% in patients with duodenal ulcer [34]. It would be of interest to study the epidemiology of H. pylori infection in the region as a whole as well as examining whether the low prevalence of H. pylori among the Langi is due to the “racial cohort” phenomenon as has been demonstrated for the Malays [33].…”

Section: Discussionmentioning

confidence: 99%

Helicobacter pylori infection in pregnant women in four districts of Uganda: role of geographic location, education and water sources

2014

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Background: The prevalence of Helicobacter pylori infection varies in relation to geography, ethnicity and socioeconomic factors. Available data on the prevalence of Helicobacter pylori infection in Uganda are not representative of the general population. We sought to describe the epidemiology of this infection in pregnant women in Uganda to provide background data for a study into the effect of H. pylori infection during pregnancy on the hematological response to iron supplementation. Methods: Using a cross-sectional design, H. pylori infection was assessed by the stool antigen test among 447 pregnant women attending antenatal care clinics in Apac, Mbale, Mbarara and Rakai Districts which are in different geographical regions in Uganda, and at Kawempe Health Center which serves a low-income densely populated area in Kampala City. Socio-demographic and household data were collected by face-to-face interviews using a questionnaire. Associations between H. pylori infection and socio-demographic and household characteristics were analyzed using logistic regression. Results: The overall prevalence of H. pylori infection was 45.2% but varied by geographical location from 18.2% in Apac District to 60.5% at Kawempe Health Centre. At 18.4%, the Langi ethnic group, who were enrolled exclusively in Apac District, had the lowest prevalence of H. pylori infection while the Gisu had the highest prevalence (58.4%). H. pylori was independently associated with enrollment at clinics not in Apac (adjusted OR = 5.68; 95% CI: 3.02-10.7) and with using water from public wells, boreholes or springs (AOR = 3.20; 95% CI: 1.19-8.61) and from rivers, lakes or streams (AOR = 5.20; 95% CI: 1.58-17.05). Urban residence (AOR = 1.71; 95% CI: 1.13-2.60) and no formal education (AOR = 1.95; 95% CI: 1.03-3.67) were also independently associated with H. pylori infection. Conclusions:The unexpected variation in the prevalence of H. pylori infection in Uganda calls for population-based studies in the region and offers an opportunity to study the transmission dynamics of H. pylori infection. The association between H. pylori infection and surface water sources for household use suggests waterborne transmission of H. pylori infection highlighting the need for concerted efforts in environmental health in communities and at the household level.

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Detection of Helicobacter Pylori in Endoscopic Biopsies in Sudan

Cited by 17 publications

References 8 publications

Helicobacter pylori in Africa: Observations on an ‘enigma within an enigma’

Helicobacter pylori in Africa: Observations on an ‘enigma within an enigma’

Molecular Phylogenetic Analysis of16S rRNASequences Identified Two lineages ofH. pyloriStrains Detected from Different Regions in Sudan Suggestive of Differential Evolution

Helicobacter pylori infection in pregnant women in four districts of Uganda: role of geographic location, education and water sources

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